Matches in SemOpenAlex for { <https://semopenalex.org/work/W1995395488> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W1995395488 endingPage "2568" @default.
- W1995395488 startingPage "2564" @default.
- W1995395488 abstract "Chitin deacetylase, the enzyme that catalyzes the hydrolysis of acetamido groups of N-acetylglucosamine in chitin, has been purified to homogeneity from mycelial extracts of the fungus Mucor rouxii and further characterized. The enzyme exhibits a low pI (approximately 3). Its apparent molecular mass was determined to be approximately 75 kDa by sodium dodecyl sulfate/polyacrylamide gel electrophoresis and approximately 80 kDa by size-exclusion chromatography, suggesting that the enzyme exists as a monomer. Carbohydrate analysis of purified chitin deacetylase revealed that the enzyme is a high-mannose glycoprotein and that its carbohydrate content is approximately 30% by weight. Chitin deacetylase is active on several chitinous substrates and chitin derivatives. The enzyme requires at least four N-acetylglucosamine residues (chitotetraose) for catalysis, and it is inhibited by carboxylic acids, particularly acetic acid. When glycol chitin (a water-soluble chitin derivative) was used as substrate, the optimum temperature for enzyme activity was determined to be approximately 50 degrees C and the optimum pH was approximately 4.5." @default.
- W1995395488 created "2016-06-24" @default.
- W1995395488 creator A5039455654 @default.
- W1995395488 creator A5043489010 @default.
- W1995395488 creator A5044951417 @default.
- W1995395488 date "1993-04-01" @default.
- W1995395488 modified "2023-10-18" @default.
- W1995395488 title "Bioconversion of chitin to chitosan: purification and characterization of chitin deacetylase from Mucor rouxii." @default.
- W1995395488 cites W1424913112 @default.
- W1995395488 cites W1508445806 @default.
- W1995395488 cites W1581490135 @default.
- W1995395488 cites W1589482850 @default.
- W1995395488 cites W1983568292 @default.
- W1995395488 cites W1991498037 @default.
- W1995395488 cites W2018289835 @default.
- W1995395488 cites W2084981024 @default.
- W1995395488 cites W2100837269 @default.
- W1995395488 cites W2102131116 @default.
- W1995395488 cites W2111301002 @default.
- W1995395488 cites W2137596882 @default.
- W1995395488 doi "https://doi.org/10.1073/pnas.90.7.2564" @default.
- W1995395488 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/46135" @default.
- W1995395488 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8464862" @default.
- W1995395488 hasPublicationYear "1993" @default.
- W1995395488 type Work @default.
- W1995395488 sameAs 1995395488 @default.
- W1995395488 citedByCount "217" @default.
- W1995395488 countsByYear W19953954882012 @default.
- W1995395488 countsByYear W19953954882013 @default.
- W1995395488 countsByYear W19953954882014 @default.
- W1995395488 countsByYear W19953954882015 @default.
- W1995395488 countsByYear W19953954882016 @default.
- W1995395488 countsByYear W19953954882017 @default.
- W1995395488 countsByYear W19953954882018 @default.
- W1995395488 countsByYear W19953954882019 @default.
- W1995395488 countsByYear W19953954882020 @default.
- W1995395488 countsByYear W19953954882021 @default.
- W1995395488 countsByYear W19953954882022 @default.
- W1995395488 countsByYear W19953954882023 @default.
- W1995395488 crossrefType "journal-article" @default.
- W1995395488 hasAuthorship W1995395488A5039455654 @default.
- W1995395488 hasAuthorship W1995395488A5043489010 @default.
- W1995395488 hasAuthorship W1995395488A5044951417 @default.
- W1995395488 hasBestOaLocation W19953954882 @default.
- W1995395488 hasConcept C181199279 @default.
- W1995395488 hasConcept C185592680 @default.
- W1995395488 hasConcept C2775923588 @default.
- W1995395488 hasConcept C2779732960 @default.
- W1995395488 hasConcept C2781088250 @default.
- W1995395488 hasConcept C43617362 @default.
- W1995395488 hasConcept C55493867 @default.
- W1995395488 hasConcept C58121356 @default.
- W1995395488 hasConceptScore W1995395488C181199279 @default.
- W1995395488 hasConceptScore W1995395488C185592680 @default.
- W1995395488 hasConceptScore W1995395488C2775923588 @default.
- W1995395488 hasConceptScore W1995395488C2779732960 @default.
- W1995395488 hasConceptScore W1995395488C2781088250 @default.
- W1995395488 hasConceptScore W1995395488C43617362 @default.
- W1995395488 hasConceptScore W1995395488C55493867 @default.
- W1995395488 hasConceptScore W1995395488C58121356 @default.
- W1995395488 hasIssue "7" @default.
- W1995395488 hasLocation W19953954881 @default.
- W1995395488 hasLocation W19953954882 @default.
- W1995395488 hasLocation W19953954883 @default.
- W1995395488 hasLocation W19953954884 @default.
- W1995395488 hasOpenAccess W1995395488 @default.
- W1995395488 hasPrimaryLocation W19953954881 @default.
- W1995395488 hasRelatedWork W167615296 @default.
- W1995395488 hasRelatedWork W1969144291 @default.
- W1995395488 hasRelatedWork W1969642539 @default.
- W1995395488 hasRelatedWork W2005959167 @default.
- W1995395488 hasRelatedWork W2014743726 @default.
- W1995395488 hasRelatedWork W2054833292 @default.
- W1995395488 hasRelatedWork W2366674522 @default.
- W1995395488 hasRelatedWork W2368826197 @default.
- W1995395488 hasRelatedWork W4220793713 @default.
- W1995395488 hasRelatedWork W4307261655 @default.
- W1995395488 hasVolume "90" @default.
- W1995395488 isParatext "false" @default.
- W1995395488 isRetracted "false" @default.
- W1995395488 magId "1995395488" @default.
- W1995395488 workType "article" @default.