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Matches in SemOpenAlex for { <https://semopenalex.org/work/W1995395663> ?p ?o ?g. }

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• W1995395663 abstract "The restriction endonuclease PvuII has been introduced as a sequence-specific cleavage module in highly-specific nucleases for gene targeting. Here, a structural reorganization of the single-chain variant of PvuII (scPvuII) was performed by circular permutation as a proof-of-concept in order to find out whether the relocated, new termini next to structural elements important for DNA recognition and catalysis could be used for the fusion with other regulatory protein domains. Three circularly permuted variants of scPvuII were obtained that all maintain the specific endonucleolytic activity of scPvuII." @default.
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• W1995395663 date "2012-05-18" @default.
• W1995395663 modified "2023-10-18" @default.
• W1995395663 title "Redesigning the single-chain variant of the restriction endonuclease PvuII by circular permutation" @default.
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• W1995395663 doi "https://doi.org/10.1016/j.febslet.2012.05.012" @default.
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