FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1995447876> ?p ?o ?g. }

• W1995447876 endingPage "12442" @default.
• W1995447876 startingPage "12432" @default.
• W1995447876 abstract "Protein-ligand interactions may lead to the formation of multiple molecular complexes in dynamic exchange, affecting the kinetic and thermodynamic characteristics of the binding equilibrium. We followed the dissociation kinetics of the transient and specific complex of an antithrombotic peptide N-acetyl-Asp(55)-Phe-Glu-Glu-Ile-Pro(60)-Glu-Glu-Tyr-Leu-Gln(65) with human prothrombin by use of (15)N NMR relaxation dispersion spectroscopy of the peptide. Every one of the five (15)N-labeled adjacent residues of the peptide exhibited apparently different kinetic exchange and relaxation behaviors, which were especially evident at different concentrations of prothrombin. Binding-induced (15)N relaxation dispersion of residues Phe(56), Glu(57), Glu(58), and Ile(59) can be fitted phenomenologically to a two-site on-and-off exchange mechanism with physically feasible relaxation and kinetic parameters obtained for residues Phe(56), Glu(58), and Ile(59), independent of the prothrombin concentration. The apparent kinetic parameters of Glu(57) show some dependence on the concentration of prothrombin and the extracted transverse relaxation rate for Glu(57) in the bound state was severalfold higher than that expected for a protein-peptide complex with a size of approximately 72 kDa. In addition, the equilibrium population of the bound peptide obtained for Glu(57) was inconsistent with those for Phe(56), Glu(58), and Ile(59) and with the prothrombin/peptide ratios used in the experiments. These discrepancies can be explained by the presence of two conformations for the peptide-protein complex exchanging at a rate of approximately 100 s(-)(1). In all, our study shows that fast dissociation of protein-peptide complexes can be studied quantitatively using peptide (15)N NMR relaxation dispersion measurements without a precise knowledge of the peptide and protein concentrations. In addition, protein titration was found to improve the accuracy of quantitative analysis and may make it possible to determine the rate of conformational changes within the protein-peptide complex." @default.
• W1995447876 created "2016-06-24" @default.
• W1995447876 creator A5010295458 @default.
• W1995447876 creator A5020939061 @default.
• W1995447876 creator A5075900560 @default.
• W1995447876 date "2003-09-17" @default.
• W1995447876 modified "2023-09-23" @default.
• W1995447876 title "Probing the Kinetic Landscape of Transient Peptide−Protein Interactions by Use of Peptide ¹⁵N NMR Relaxation Dispersion Spectroscopy: Binding of an Antithrombin Peptide to Human Prothrombin" @default.
• W1995447876 cites W1563420247 @default.
• W1995447876 cites W1595995045 @default.
• W1995447876 cites W1661670649 @default.
• W1995447876 cites W1966161117 @default.
• W1995447876 cites W1967138971 @default.
• W1995447876 cites W1971388680 @default.
• W1995447876 cites W1972091733 @default.
• W1995447876 cites W1972459558 @default.
• W1995447876 cites W1972647870 @default.
• W1995447876 cites W1980040760 @default.
• W1995447876 cites W1981342080 @default.
• W1995447876 cites W1987528091 @default.
• W1995447876 cites W1992888087 @default.
• W1995447876 cites W1994939140 @default.
• W1995447876 cites W2001638826 @default.
• W1995447876 cites W2002097614 @default.
• W1995447876 cites W2003978987 @default.
• W1995447876 cites W2004967944 @default.
• W1995447876 cites W2005863578 @default.
• W1995447876 cites W2008674600 @default.
• W1995447876 cites W2009119119 @default.
• W1995447876 cites W2023028613 @default.
• W1995447876 cites W2034754052 @default.
• W1995447876 cites W2034941317 @default.
• W1995447876 cites W2044999527 @default.
• W1995447876 cites W2050908210 @default.
• W1995447876 cites W2053160952 @default.
• W1995447876 cites W2056286174 @default.
• W1995447876 cites W2070772022 @default.
• W1995447876 cites W2074490799 @default.
• W1995447876 cites W2088173883 @default.
• W1995447876 cites W2089320509 @default.
• W1995447876 cites W2091223555 @default.
• W1995447876 cites W2112605368 @default.
• W1995447876 cites W2126818937 @default.
• W1995447876 cites W2127073449 @default.
• W1995447876 cites W2136087655 @default.
• W1995447876 cites W2136828798 @default.
• W1995447876 cites W2145061993 @default.
• W1995447876 cites W2158609362 @default.
• W1995447876 cites W2165849976 @default.
• W1995447876 cites W2169821755 @default.
• W1995447876 cites W218645508 @default.
• W1995447876 cites W2416871144 @default.
• W1995447876 cites W2949307891 @default.
• W1995447876 cites W4252208796 @default.
• W1995447876 cites W4256738009 @default.
• W1995447876 doi "https://doi.org/10.1021/ja021238l" @default.
• W1995447876 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14531686" @default.
• W1995447876 hasPublicationYear "2003" @default.
• W1995447876 type Work @default.
• W1995447876 sameAs 1995447876 @default.
• W1995447876 citedByCount "39" @default.
• W1995447876 countsByYear W19954478762012 @default.
• W1995447876 countsByYear W19954478762014 @default.
• W1995447876 countsByYear W19954478762016 @default.
• W1995447876 countsByYear W19954478762017 @default.
• W1995447876 countsByYear W19954478762018 @default.
• W1995447876 countsByYear W19954478762019 @default.
• W1995447876 countsByYear W19954478762021 @default.
• W1995447876 crossrefType "journal-article" @default.
• W1995447876 hasAuthorship W1995447876A5010295458 @default.
• W1995447876 hasAuthorship W1995447876A5020939061 @default.
• W1995447876 hasAuthorship W1995447876A5075900560 @default.
• W1995447876 hasConcept C144024400 @default.
• W1995447876 hasConcept C149923435 @default.
• W1995447876 hasConcept C15744967 @default.
• W1995447876 hasConcept C178790620 @default.
• W1995447876 hasConcept C185592680 @default.
• W1995447876 hasConcept C2776029896 @default.
• W1995447876 hasConcept C2779281246 @default.
• W1995447876 hasConcept C2779546866 @default.
• W1995447876 hasConcept C2908647359 @default.
• W1995447876 hasConcept C55493867 @default.
• W1995447876 hasConcept C66974803 @default.
• W1995447876 hasConcept C71240020 @default.
• W1995447876 hasConcept C77805123 @default.
• W1995447876 hasConcept C8010536 @default.
• W1995447876 hasConceptScore W1995447876C144024400 @default.
• W1995447876 hasConceptScore W1995447876C149923435 @default.
• W1995447876 hasConceptScore W1995447876C15744967 @default.
• W1995447876 hasConceptScore W1995447876C178790620 @default.
• W1995447876 hasConceptScore W1995447876C185592680 @default.
• W1995447876 hasConceptScore W1995447876C2776029896 @default.
• W1995447876 hasConceptScore W1995447876C2779281246 @default.
• W1995447876 hasConceptScore W1995447876C2779546866 @default.
• W1995447876 hasConceptScore W1995447876C2908647359 @default.
• W1995447876 hasConceptScore W1995447876C55493867 @default.
• W1995447876 hasConceptScore W1995447876C66974803 @default.
• W1995447876 hasConceptScore W1995447876C71240020 @default.

• next