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Matches in SemOpenAlex for { <https://semopenalex.org/work/W1995504709> ?p ?o ?g. }

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• W1995504709 abstract "Numerous studies of S-glutathionylation of cysteine thiols indicate that this protein modification plays a key role in redox regulation of proteins. To facilitate the study of protein S-glutathionylation, we developed a synthesis and purification to produce milligram quantities of fluorescein-labeled glutathione. The amino terminus of the glutathione tripeptide reacted with fluorescein isothiocyanate readily in ammonium bicarbonate. Purification by solid phase extraction on C8 and C18 columns separated excess reactants from desired products. Both oxidized and reduced fluorescein-labeled glutathione reacted with a variety of thiol-containing proteins to yield fluorescent proteins." @default.
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• W1995504709 date "2010-07-01" @default.
• W1995504709 modified "2023-10-16" @default.
• W1995504709 title "Fluorescein-labeled glutathione to study protein S-glutathionylation" @default.
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• W1995504709 doi "https://doi.org/10.1016/j.ab.2010.02.006" @default.
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