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- W1995514009 abstract "Abstract Depletion of host specific alloreactive T-cells from donor lymphocytes is an attractive tool for donor lymphocyte infusions after stem cell grafting. We intended to establish a preclinical method to evaluate effectiveness and safety of this approach. The skin explant model is a powerful method to predict the risk of GvHD in the HLA-identical setting, and we adapted this model to the HLA-haploidentical situation. Unmanipulated donor T-cells are compared with donor T-cells following depletion of host specific T-cells. Prior to depletion donor MNC are incubated in the presence of irradiated recipient MNC and after a period of 5 days activated T-cells, positive for CD25, CD69, CD71 and HLA-DR, are magnetically removed (VarioMacs, Miltenyi). A repeat MLC of unmanipulated donor cells. Fresh skin biopsies are divided into pieces of 1 - 2 mm diameter and incubated with 10 6 cultured T-cells in 20% autologous serum for 72 hours, fixed in formaldehyde and stained with haematoxylin-eosin. Skin alterations are graded according to Learner et al. In 3 separate experiments undepleted cells caused subepidermal cleft formation corresponding to a grade III to IV GvHD reaction. In contrast, depleted cells showed only grade I alterations, as similarly observed in medium control. Our preliminary data suggest that this approach could represent a useful tool to determine effectiveness of magnetic depletion of alloreactive T-cells and be valuable to assess the safety of these cells prior to infusion." @default.
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- W1995514009 date "2000-07-01" @default.
- W1995514009 modified "2023-10-18" @default.
- W1995514009 title "Skin-explant model to evaluate effectiveness of depletion of hla-alloreactive t-cells" @default.
- W1995514009 doi "https://doi.org/10.1016/s0301-472x(00)00406-9" @default.
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