FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1995524921> ?p ?o ?g. }

• W1995524921 endingPage "941" @default.
• W1995524921 startingPage "935" @default.
• W1995524921 abstract "Sample preparation, especially protein and peptide fractionation prior to identification by mass spectrometry (MS), is typically applied to reduce sample complexity. The second key element in this process is proteolytic digestion, which is performed most often with trypsin. Optimization of this step is an important factor in order to achieve both speed and better performance of proteomic analysis, and tryptic digestion prior to the MS analysis has been a topic of many studies. To date, only a few studies have paid attention to the negative interaction between the proteolytic enzyme and sample components, and sample losses caused by these interactions. In this study, we demonstrated impaired activity after in solution tryptic digestion of plasma proteins caused by a potent trypsin inhibitor family, inter-alpha inhibitor proteins. Sample boiling followed by gel electrophoretic separation and in-gel digestion drastically improved both the number of identified proteins and the sequence coverage in subsequent LC-ESI-MS/MS. The present investigations show that a thorough validation is necessary when in solution digestion followed by LC-MS analysis of complex biological samples is performed. The parallel use of two or more different mass spectrometers can also yield additional information and contribute to further method validation." @default.
• W1995524921 created "2016-06-24" @default.
• W1995524921 creator A5004207431 @default.
• W1995524921 creator A5034818646 @default.
• W1995524921 creator A5039497322 @default.
• W1995524921 creator A5045423823 @default.
• W1995524921 creator A5052531267 @default.
• W1995524921 creator A5087447670 @default.
• W1995524921 date "2011-06-01" @default.
• W1995524921 modified "2023-10-04" @default.
• W1995524921 title "Protease inhibitors as possible pitfalls in proteomic analyses of complex biological samples" @default.
• W1995524921 cites W1976868037 @default.
• W1995524921 cites W1986737545 @default.
• W1995524921 cites W1991963460 @default.
• W1995524921 cites W2002134711 @default.
• W1995524921 cites W2005818888 @default.
• W1995524921 cites W2007681573 @default.
• W1995524921 cites W2011628535 @default.
• W1995524921 cites W2014269786 @default.
• W1995524921 cites W2018075711 @default.
• W1995524921 cites W2026465178 @default.
• W1995524921 cites W2034356373 @default.
• W1995524921 cites W2036555963 @default.
• W1995524921 cites W2061188066 @default.
• W1995524921 cites W2062050705 @default.
• W1995524921 cites W2071655317 @default.
• W1995524921 cites W2071857608 @default.
• W1995524921 cites W2072161393 @default.
• W1995524921 cites W2072259643 @default.
• W1995524921 cites W2074157555 @default.
• W1995524921 cites W2077956068 @default.
• W1995524921 cites W2078483808 @default.
• W1995524921 cites W2084849510 @default.
• W1995524921 cites W2096057003 @default.
• W1995524921 cites W2105869274 @default.
• W1995524921 cites W2109582216 @default.
• W1995524921 cites W2134852489 @default.
• W1995524921 cites W2171652500 @default.
• W1995524921 doi "https://doi.org/10.1016/j.jprot.2011.02.010" @default.
• W1995524921 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3107918" @default.
• W1995524921 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21333769" @default.
• W1995524921 hasPublicationYear "2011" @default.
• W1995524921 type Work @default.
• W1995524921 sameAs 1995524921 @default.
• W1995524921 citedByCount "19" @default.
• W1995524921 countsByYear W19955249212012 @default.
• W1995524921 countsByYear W19955249212013 @default.
• W1995524921 countsByYear W19955249212014 @default.
• W1995524921 countsByYear W19955249212015 @default.
• W1995524921 countsByYear W19955249212017 @default.
• W1995524921 countsByYear W19955249212018 @default.
• W1995524921 countsByYear W19955249212020 @default.
• W1995524921 countsByYear W19955249212021 @default.
• W1995524921 countsByYear W19955249212022 @default.
• W1995524921 countsByYear W19955249212023 @default.
• W1995524921 crossrefType "journal-article" @default.
• W1995524921 hasAuthorship W1995524921A5004207431 @default.
• W1995524921 hasAuthorship W1995524921A5034818646 @default.
• W1995524921 hasAuthorship W1995524921A5039497322 @default.
• W1995524921 hasAuthorship W1995524921A5045423823 @default.
• W1995524921 hasAuthorship W1995524921A5052531267 @default.
• W1995524921 hasAuthorship W1995524921A5087447670 @default.
• W1995524921 hasBestOaLocation W19955249212 @default.
• W1995524921 hasConcept C104317684 @default.
• W1995524921 hasConcept C104397665 @default.
• W1995524921 hasConcept C112979427 @default.
• W1995524921 hasConcept C162356407 @default.
• W1995524921 hasConcept C181199279 @default.
• W1995524921 hasConcept C185592680 @default.
• W1995524921 hasConcept C202157981 @default.
• W1995524921 hasConcept C2776714187 @default.
• W1995524921 hasConcept C2778576202 @default.
• W1995524921 hasConcept C2780340462 @default.
• W1995524921 hasConcept C2781307694 @default.
• W1995524921 hasConcept C2781357212 @default.
• W1995524921 hasConcept C3019223276 @default.
• W1995524921 hasConcept C40684141 @default.
• W1995524921 hasConcept C43617362 @default.
• W1995524921 hasConcept C46111723 @default.
• W1995524921 hasConcept C55493867 @default.
• W1995524921 hasConcept C97428945 @default.
• W1995524921 hasConceptScore W1995524921C104317684 @default.
• W1995524921 hasConceptScore W1995524921C104397665 @default.
• W1995524921 hasConceptScore W1995524921C112979427 @default.
• W1995524921 hasConceptScore W1995524921C162356407 @default.
• W1995524921 hasConceptScore W1995524921C181199279 @default.
• W1995524921 hasConceptScore W1995524921C185592680 @default.
• W1995524921 hasConceptScore W1995524921C202157981 @default.
• W1995524921 hasConceptScore W1995524921C2776714187 @default.
• W1995524921 hasConceptScore W1995524921C2778576202 @default.
• W1995524921 hasConceptScore W1995524921C2780340462 @default.
• W1995524921 hasConceptScore W1995524921C2781307694 @default.
• W1995524921 hasConceptScore W1995524921C2781357212 @default.
• W1995524921 hasConceptScore W1995524921C3019223276 @default.
• W1995524921 hasConceptScore W1995524921C40684141 @default.
• W1995524921 hasConceptScore W1995524921C43617362 @default.
• W1995524921 hasConceptScore W1995524921C46111723 @default.
• W1995524921 hasConceptScore W1995524921C55493867 @default.

• next