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- W1995565443 abstract "The role of deoxyribonucleic acid (DNA) in the growth of Rous sarcoma virus (RSV) was examined utilizing actinomycin and 5-bromodeoxyuridine (BUDR). Actinomycin D (3 μg/ml) suppressed synthesis of RSV in chick embryo cells within 4 hours after addition and prior to any obvious change in the microscopic morphology of the cells. Growth of vesicular stomatitis virus, an RNA-containing virus, was not inhibited by actinomycin. BUDR also inhibits growth of RSV. Cells pretreated with BUDR could support the growth of RSV if BUDR was removed, but exposure of pretreated cells to additional BUDR inhibited virus growth tenfold. Cultures treated with BUDR in which no cellular growth was detectable and DNA synthesis was minimal could still support the growth of RSV. Inhibition of RSV growth by BUDR was reversible by thymidine. These results indicate that the growth of RSV requires the synthesis of a new DNA, and the possibility arises that the RSV particle may contain a DNA moiety." @default.
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- W1995565443 title "The role of deoxyribonucleic acid in the synthesis of Rous sarcoma virus" @default.
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- W1995565443 doi "https://doi.org/10.1016/0042-6822(64)90067-4" @default.
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