Matches in SemOpenAlex for { <https://semopenalex.org/work/W1995591507> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W1995591507 endingPage "214" @default.
- W1995591507 startingPage "191" @default.
- W1995591507 abstract "In today's high-throughput screening (HTS) environment, an increasing number of assay detection technologies are routinely utilized in lead finding programs. Because of the relatively broad applicability of several of these technologies, one is often faced with a choice of which technology to utilize for a specific assay. The aim of this study was to address the question of whether the same compounds would be identified from screening a set of samples in three different versions of an HTS assay. Here, three different versions of a tyrosine kinase assay were established using scintillation proximity assay (SPA), homogeneous time-resolved fluorescence resonance energy transfer (HTR-FRET), and fluorescence polarization (FP) technologies. In this study, 30,000 compounds were evaluated in each version of the kinase assay in primary screening, deconvolution, and dose-response experiments. From this effort, there was only a small degree of overlap of active compounds identified subsequent to the deconvolution experiment. When all active compounds were then profiled in all three assays, 100 and 101 active compounds were identified in the HTR-FRET and FP assays, respectively. In contrast, 40 compounds were identified in the SPA version of the kinase assay, whereas all of these compounds were detected in the HTR-FRET assay only 35 were active in the FP assay. Although there was good correlation between the IC(50) values obtained in the HTR-FRET and FP assays, poor correlations were obtained with the IC(50) values obtained in the SPA assay. These findings suggest that significant differences can be observed from HTS depending on the assay technology that is utilized, particularly in assays with high hit rates." @default.
- W1995591507 created "2016-06-24" @default.
- W1995591507 creator A5015803414 @default.
- W1995591507 creator A5022991259 @default.
- W1995591507 creator A5026095267 @default.
- W1995591507 creator A5030181277 @default.
- W1995591507 creator A5038683160 @default.
- W1995591507 creator A5071144036 @default.
- W1995591507 date "2002-06-01" @default.
- W1995591507 modified "2023-10-10" @default.
- W1995591507 title "Comparison of Assay Technologies for a Tyrosine Kinase Assay Generates Different Results in High Throughput Screening" @default.
- W1995591507 cites W1599075286 @default.
- W1995591507 cites W1964381872 @default.
- W1995591507 cites W1991001144 @default.
- W1995591507 cites W1996437478 @default.
- W1995591507 cites W2019590054 @default.
- W1995591507 cites W2029898876 @default.
- W1995591507 cites W2030825535 @default.
- W1995591507 cites W2031962217 @default.
- W1995591507 cites W2084020594 @default.
- W1995591507 cites W2142115916 @default.
- W1995591507 doi "https://doi.org/10.1177/108705710200700304" @default.
- W1995591507 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12097183" @default.
- W1995591507 hasPublicationYear "2002" @default.
- W1995591507 type Work @default.
- W1995591507 sameAs 1995591507 @default.
- W1995591507 citedByCount "84" @default.
- W1995591507 countsByYear W19955915072012 @default.
- W1995591507 countsByYear W19955915072013 @default.
- W1995591507 countsByYear W19955915072014 @default.
- W1995591507 countsByYear W19955915072015 @default.
- W1995591507 countsByYear W19955915072016 @default.
- W1995591507 countsByYear W19955915072017 @default.
- W1995591507 countsByYear W19955915072018 @default.
- W1995591507 countsByYear W19955915072021 @default.
- W1995591507 crossrefType "journal-article" @default.
- W1995591507 hasAuthorship W1995591507A5015803414 @default.
- W1995591507 hasAuthorship W1995591507A5022991259 @default.
- W1995591507 hasAuthorship W1995591507A5026095267 @default.
- W1995591507 hasAuthorship W1995591507A5030181277 @default.
- W1995591507 hasAuthorship W1995591507A5038683160 @default.
- W1995591507 hasAuthorship W1995591507A5071144036 @default.
- W1995591507 hasBestOaLocation W19955915071 @default.
- W1995591507 hasConcept C111357860 @default.
- W1995591507 hasConcept C11413529 @default.
- W1995591507 hasConcept C121332964 @default.
- W1995591507 hasConcept C174576160 @default.
- W1995591507 hasConcept C185592680 @default.
- W1995591507 hasConcept C41008148 @default.
- W1995591507 hasConcept C41625074 @default.
- W1995591507 hasConcept C42362537 @default.
- W1995591507 hasConcept C51323132 @default.
- W1995591507 hasConcept C55493867 @default.
- W1995591507 hasConcept C62478195 @default.
- W1995591507 hasConcept C62520636 @default.
- W1995591507 hasConcept C70721500 @default.
- W1995591507 hasConcept C86803240 @default.
- W1995591507 hasConcept C91881484 @default.
- W1995591507 hasConcept C96305047 @default.
- W1995591507 hasConceptScore W1995591507C111357860 @default.
- W1995591507 hasConceptScore W1995591507C11413529 @default.
- W1995591507 hasConceptScore W1995591507C121332964 @default.
- W1995591507 hasConceptScore W1995591507C174576160 @default.
- W1995591507 hasConceptScore W1995591507C185592680 @default.
- W1995591507 hasConceptScore W1995591507C41008148 @default.
- W1995591507 hasConceptScore W1995591507C41625074 @default.
- W1995591507 hasConceptScore W1995591507C42362537 @default.
- W1995591507 hasConceptScore W1995591507C51323132 @default.
- W1995591507 hasConceptScore W1995591507C55493867 @default.
- W1995591507 hasConceptScore W1995591507C62478195 @default.
- W1995591507 hasConceptScore W1995591507C62520636 @default.
- W1995591507 hasConceptScore W1995591507C70721500 @default.
- W1995591507 hasConceptScore W1995591507C86803240 @default.
- W1995591507 hasConceptScore W1995591507C91881484 @default.
- W1995591507 hasConceptScore W1995591507C96305047 @default.
- W1995591507 hasIssue "3" @default.
- W1995591507 hasLocation W19955915071 @default.
- W1995591507 hasLocation W19955915072 @default.
- W1995591507 hasOpenAccess W1995591507 @default.
- W1995591507 hasPrimaryLocation W19955915071 @default.
- W1995591507 hasRelatedWork W1964381872 @default.
- W1995591507 hasRelatedWork W2045014276 @default.
- W1995591507 hasRelatedWork W2045833870 @default.
- W1995591507 hasRelatedWork W2146292829 @default.
- W1995591507 hasRelatedWork W2343443025 @default.
- W1995591507 hasRelatedWork W2887248072 @default.
- W1995591507 hasRelatedWork W2972649332 @default.
- W1995591507 hasRelatedWork W2999570684 @default.
- W1995591507 hasRelatedWork W3155708370 @default.
- W1995591507 hasRelatedWork W94085293 @default.
- W1995591507 hasVolume "7" @default.
- W1995591507 isParatext "false" @default.
- W1995591507 isRetracted "false" @default.
- W1995591507 magId "1995591507" @default.
- W1995591507 workType "article" @default.