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• W1995594480 abstract "Abstract A rapid and sensitive high-performance liquid chromatographic assay for cytosine and cytidine deaminases, based upon the chromatographic separation and subsequent ultraviolet detection of enzymatically liberated uracil and uridine, was developed. Using cell-free extract from Escherichia coli the enzymes can be assayed with incubation times of 30 min or less. Reversed-phase separation of products from substrates was accomplished by isocratic elution with monobasic ammonium phosphate buffer at pH 3.5. The assay is fast and reproducible with little or no interference from competing reactions in cell extracts. It is sensitive and can concomitantly detect nanomole changes in the concentration of substrate and product. It is faster, more sensitive, and requires fewer sample manipulations than standard spectrophotometric and radiometric methods of analysis." @default.
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• W1995594480 date "1990-07-01" @default.
• W1995594480 modified "2023-09-24" @default.
• W1995594480 title "Assay of cytosine and cytidine by means of reversed-phase high-performance liquid chromatography" @default.
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• W1995594480 doi "https://doi.org/10.1016/s0021-9673(01)89505-4" @default.
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