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- W1995595526 abstract "Vascular endothelial growth factor (VEGF) is an angiogenic factor and a potent stimulator of microvascular permeability. It is a mitogen specific for endothelial cells. The expression of VEGF and its two receptors, Flk-1 and Flt-1, is pivotal for the proper formation of blood vessels in embryogenesis as shown by gene-targeting experiments. Interestingly, the loss of even a single allele of VEGF led to embryonic lethality between day E9.5 and day E10.5 in the mouse. To assess the role of VEGF during embryonic development we decided to tag VEGF expression with LacZ, by inserting an IRES (internal ribosome entry site)-LacZ reporter cassette into the 3' untranslated region of the gene. This alteration enabled us to monitor VEGF expression throughout embryonic development at single-cell resolution. beta-Galactosidase expression from the altered VEGF locus was first observed prior to gastrulation and was detectable at all stages of vascular development in the embryo. Later, the specific cellular distribution and the level of VEGF expression indicated its pleiotropic role in development. High expression levels seemed to be associated with vasculogenesis and permeability, whereas lower levels were associated with angiogenesis and cell migration. In addition, we found VEGF expression in a subtype of endothelial cells present in the endocardium. We believe that the LacZ-tagged allele we have generated offers a precise means of detecting VEGF expression under a variety of physiological and pathological conditions." @default.
- W1995595526 created "2016-06-24" @default.
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- W1995595526 date "1999-08-01" @default.
- W1995595526 modified "2023-10-14" @default.
- W1995595526 title "Multiple Developmental Roles of VEGF Suggested by a LacZ-Tagged Allele" @default.
- W1995595526 cites W1546150158 @default.
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- W1995595526 cites W1807212217 @default.
- W1995595526 cites W1807482481 @default.
- W1995595526 cites W1838110840 @default.
- W1995595526 cites W1957871175 @default.
- W1995595526 cites W1966697925 @default.
- W1995595526 cites W1972320565 @default.
- W1995595526 cites W1974951139 @default.
- W1995595526 cites W1980755389 @default.
- W1995595526 cites W1983763042 @default.
- W1995595526 cites W1985712533 @default.
- W1995595526 cites W1986559037 @default.
- W1995595526 cites W1988688040 @default.
- W1995595526 cites W2003598961 @default.
- W1995595526 cites W2011584733 @default.
- W1995595526 cites W2012390831 @default.
- W1995595526 cites W2017596496 @default.
- W1995595526 cites W2022653793 @default.
- W1995595526 cites W2024601523 @default.
- W1995595526 cites W2026530967 @default.
- W1995595526 cites W2029877619 @default.
- W1995595526 cites W2036751011 @default.
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- W1995595526 cites W2039525141 @default.
- W1995595526 cites W2042130990 @default.
- W1995595526 cites W2043863730 @default.
- W1995595526 cites W2045247659 @default.
- W1995595526 cites W2046516822 @default.
- W1995595526 cites W2048185125 @default.
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- W1995595526 cites W2067817921 @default.
- W1995595526 cites W2084506573 @default.
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- W1995595526 cites W2092807134 @default.
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- W1995595526 doi "https://doi.org/10.1006/dbio.1999.9355" @default.
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