FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1995651421> ?p ?o ?g. }

• W1995651421 endingPage "10746" @default.
• W1995651421 startingPage "10739" @default.
• W1995651421 abstract "Nucleotide excision repair (NER) is a repair pathway that removes a variety of bulky DNA lesions in both prokaryotic and eukaryotic cells. The perturbation of DNA helix structure caused by the oxidative intrastrand lesions could render them good substrates for the NER pathway. Here we employed Escherichia coli NER enzymes, i.e., UvrA, UvrB, and UvrC, to examine the incision efficiency of duplex DNA carrying three different oxidative intrastrand cross-link lesions, that is, G[8-5]C, G[8-5m]mC, and G[8-5m]T, and two dithymine photoproducts, namely, the cis,syn-cyclobutane pyrimidine dimer (T[c,s]T) and the pyrimidine(6-4)pyrimidone product (T[6-4]T). Our results showed that T[6-4]T was the best substrate for UvrA binding, followed by G[8-5]C, G[8-5m]mC, and G[8-5m]T, and then by T[c,s]T. The efficiencies of the UvrABC incisions of these lesions were consistent with their UvrA binding affinities: the stronger the binding to UvrA, the higher the rate of incision. In addition, flanking DNA sequences appeared to have little effect on the binding affinity of UvrA for G[8-5]C as AG[8-5]CA was only slightly preferred over CG[8-5]CG. Consistently, these two sequences exhibited almost no difference in incision rates. Furthermore, we investigated the thermal stability of dodecameric duplexes containing G[8-5m]mC or G[8-5m]T, and our results revealed that these two lesions destabilized the duplex, due to an increase in the free energy for duplex formation at 37 degrees C, by approximately 5.4 and 3.6 kcal/mol, respectively. The destabilizations to the DNA helix caused by those lesions, for the most part, are correlated with the binding affinities of UvrA and incision rates of UvrABC. Taken together, the results from this study suggest that oxidative intrastrand lesions might be substrates for NER enzymes in vivo." @default.
• W1995651421 created "2016-06-24" @default.
• W1995651421 creator A5004817105 @default.
• W1995651421 creator A5025279909 @default.
• W1995651421 creator A5039723064 @default.
• W1995651421 creator A5050784785 @default.
• W1995651421 creator A5072209268 @default.
• W1995651421 creator A5079396639 @default.
• W1995651421 date "2006-08-09" @default.
• W1995651421 modified "2023-10-18" @default.
• W1995651421 title "Recognition and Incision of Oxidative Intrastrand Cross-Link Lesions by UvrABC Nuclease" @default.
• W1995651421 cites W1483750611 @default.
• W1995651421 cites W1498040952 @default.
• W1995651421 cites W1507190685 @default.
• W1995651421 cites W1778709156 @default.
• W1995651421 cites W1966509172 @default.
• W1995651421 cites W1969615892 @default.
• W1995651421 cites W1974845893 @default.
• W1995651421 cites W1974989751 @default.
• W1995651421 cites W1979562269 @default.
• W1995651421 cites W1994384654 @default.
• W1995651421 cites W1994875540 @default.
• W1995651421 cites W1997060740 @default.
• W1995651421 cites W2001257943 @default.
• W1995651421 cites W2010777360 @default.
• W1995651421 cites W2012126538 @default.
• W1995651421 cites W2027183816 @default.
• W1995651421 cites W2030666445 @default.
• W1995651421 cites W2037525065 @default.
• W1995651421 cites W2039466739 @default.
• W1995651421 cites W2040683271 @default.
• W1995651421 cites W2052991677 @default.
• W1995651421 cites W2059048411 @default.
• W1995651421 cites W2059708498 @default.
• W1995651421 cites W2063240589 @default.
• W1995651421 cites W2063812569 @default.
• W1995651421 cites W2073586988 @default.
• W1995651421 cites W2074021681 @default.
• W1995651421 cites W2076988995 @default.
• W1995651421 cites W2085474714 @default.
• W1995651421 cites W2101745429 @default.
• W1995651421 cites W2101861123 @default.
• W1995651421 cites W2112828037 @default.
• W1995651421 cites W2113912029 @default.
• W1995651421 cites W2117808727 @default.
• W1995651421 cites W2123358328 @default.
• W1995651421 cites W2137983089 @default.
• W1995651421 cites W2172916891 @default.
• W1995651421 cites W2327534068 @default.
• W1995651421 cites W4231771393 @default.
• W1995651421 doi "https://doi.org/10.1021/bi060423z" @default.
• W1995651421 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2533692" @default.
• W1995651421 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16939226" @default.
• W1995651421 hasPublicationYear "2006" @default.
• W1995651421 type Work @default.
• W1995651421 sameAs 1995651421 @default.
• W1995651421 citedByCount "42" @default.
• W1995651421 countsByYear W19956514212012 @default.
• W1995651421 countsByYear W19956514212013 @default.
• W1995651421 countsByYear W19956514212014 @default.
• W1995651421 countsByYear W19956514212015 @default.
• W1995651421 countsByYear W19956514212016 @default.
• W1995651421 countsByYear W19956514212017 @default.
• W1995651421 countsByYear W19956514212018 @default.
• W1995651421 countsByYear W19956514212019 @default.
• W1995651421 crossrefType "journal-article" @default.
• W1995651421 hasAuthorship W1995651421A5004817105 @default.
• W1995651421 hasAuthorship W1995651421A5025279909 @default.
• W1995651421 hasAuthorship W1995651421A5039723064 @default.
• W1995651421 hasAuthorship W1995651421A5050784785 @default.
• W1995651421 hasAuthorship W1995651421A5072209268 @default.
• W1995651421 hasAuthorship W1995651421A5079396639 @default.
• W1995651421 hasBestOaLocation W19956514212 @default.
• W1995651421 hasConcept C104317684 @default.
• W1995651421 hasConcept C104451858 @default.
• W1995651421 hasConcept C134935766 @default.
• W1995651421 hasConcept C153911025 @default.
• W1995651421 hasConcept C167975912 @default.
• W1995651421 hasConcept C178790620 @default.
• W1995651421 hasConcept C185592680 @default.
• W1995651421 hasConcept C2777271071 @default.
• W1995651421 hasConcept C2779546866 @default.
• W1995651421 hasConcept C2780377690 @default.
• W1995651421 hasConcept C2780378348 @default.
• W1995651421 hasConcept C547475151 @default.
• W1995651421 hasConcept C552990157 @default.
• W1995651421 hasConcept C55493867 @default.
• W1995651421 hasConcept C71240020 @default.
• W1995651421 hasConcept C86803240 @default.
• W1995651421 hasConcept C99611785 @default.
• W1995651421 hasConceptScore W1995651421C104317684 @default.
• W1995651421 hasConceptScore W1995651421C104451858 @default.
• W1995651421 hasConceptScore W1995651421C134935766 @default.
• W1995651421 hasConceptScore W1995651421C153911025 @default.
• W1995651421 hasConceptScore W1995651421C167975912 @default.
• W1995651421 hasConceptScore W1995651421C178790620 @default.
• W1995651421 hasConceptScore W1995651421C185592680 @default.
• W1995651421 hasConceptScore W1995651421C2777271071 @default.

• next