Matches in SemOpenAlex for { <https://semopenalex.org/work/W1995705269> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W1995705269 endingPage "5454" @default.
- W1995705269 startingPage "5446" @default.
- W1995705269 abstract "The use of substrate analogues as inhibitors provides a way to understand and manipulate enzyme function. Here we report two 1 A resolution crystal structures of liver alcohol dehydrogenase in complex with NADH and two inhibitors: dimethyl sulfoxide and isobutyramide. Both structures present a dynamic state of inhibition. In the dimethyl sulfoxide complex structure, the inhibitor is caught in transition on its way to the active site using a flash-freezing protocol and a cadmium-substituted enzyme. One inhibitor molecule is partly located in the first and partly in the second coordination sphere of the active site metal. A hydroxide ion bound to the active site metal lies close to the pyridine ring of NADH, which is puckered in a twisted boat conformation. The cadmium ion is coordinated by both the hydroxide ion and the inhibitor molecule, providing structural evidence of a coordination switch at the active site metal ion. The structure of the isobutyramide complex reveals the partial formation of an adduct between the isobutyramide inhibitor and NADH. It provides evidence of the contribution of a shift from the keto to the enol tautomer during aldehyde reduction. The different positions of the inhibitors further refine the knowledge of the dynamics of the enzyme mechanism and explain how the crowded active site can facilitate the presence of a substrate and a metal-bound hydroxide ion." @default.
- W1995705269 created "2016-06-24" @default.
- W1995705269 creator A5001883169 @default.
- W1995705269 creator A5009748356 @default.
- W1995705269 creator A5015434460 @default.
- W1995705269 creator A5041541957 @default.
- W1995705269 creator A5051148950 @default.
- W1995705269 creator A5059029744 @default.
- W1995705269 date "2007-04-13" @default.
- W1995705269 modified "2023-09-27" @default.
- W1995705269 title "Structural Evidence for a Ligand Coordination Switch in Liver Alcohol Dehydrogenase" @default.
- W1995705269 cites W132943569 @default.
- W1995705269 cites W1561207830 @default.
- W1995705269 cites W1585301053 @default.
- W1995705269 cites W1598075072 @default.
- W1995705269 cites W1991588154 @default.
- W1995705269 cites W2052928475 @default.
- W1995705269 cites W2061619626 @default.
- W1995705269 cites W2103797225 @default.
- W1995705269 cites W2127552463 @default.
- W1995705269 cites W2135839939 @default.
- W1995705269 doi "https://doi.org/10.1021/bi6023594" @default.
- W1995705269 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17429946" @default.
- W1995705269 hasPublicationYear "2007" @default.
- W1995705269 type Work @default.
- W1995705269 sameAs 1995705269 @default.
- W1995705269 citedByCount "44" @default.
- W1995705269 countsByYear W19957052692012 @default.
- W1995705269 countsByYear W19957052692013 @default.
- W1995705269 countsByYear W19957052692014 @default.
- W1995705269 countsByYear W19957052692015 @default.
- W1995705269 countsByYear W19957052692016 @default.
- W1995705269 countsByYear W19957052692017 @default.
- W1995705269 countsByYear W19957052692018 @default.
- W1995705269 countsByYear W19957052692019 @default.
- W1995705269 countsByYear W19957052692020 @default.
- W1995705269 countsByYear W19957052692021 @default.
- W1995705269 countsByYear W19957052692022 @default.
- W1995705269 countsByYear W19957052692023 @default.
- W1995705269 crossrefType "journal-article" @default.
- W1995705269 hasAuthorship W1995705269A5001883169 @default.
- W1995705269 hasAuthorship W1995705269A5009748356 @default.
- W1995705269 hasAuthorship W1995705269A5015434460 @default.
- W1995705269 hasAuthorship W1995705269A5041541957 @default.
- W1995705269 hasAuthorship W1995705269A5051148950 @default.
- W1995705269 hasAuthorship W1995705269A5059029744 @default.
- W1995705269 hasConcept C11111787 @default.
- W1995705269 hasConcept C111233374 @default.
- W1995705269 hasConcept C115624301 @default.
- W1995705269 hasConcept C116569031 @default.
- W1995705269 hasConcept C161790260 @default.
- W1995705269 hasConcept C170493617 @default.
- W1995705269 hasConcept C178790620 @default.
- W1995705269 hasConcept C181199279 @default.
- W1995705269 hasConcept C185592680 @default.
- W1995705269 hasConcept C2775896084 @default.
- W1995705269 hasConcept C2777344606 @default.
- W1995705269 hasConcept C2777495281 @default.
- W1995705269 hasConcept C41183919 @default.
- W1995705269 hasConcept C55493867 @default.
- W1995705269 hasConcept C71240020 @default.
- W1995705269 hasConcept C8010536 @default.
- W1995705269 hasConceptScore W1995705269C11111787 @default.
- W1995705269 hasConceptScore W1995705269C111233374 @default.
- W1995705269 hasConceptScore W1995705269C115624301 @default.
- W1995705269 hasConceptScore W1995705269C116569031 @default.
- W1995705269 hasConceptScore W1995705269C161790260 @default.
- W1995705269 hasConceptScore W1995705269C170493617 @default.
- W1995705269 hasConceptScore W1995705269C178790620 @default.
- W1995705269 hasConceptScore W1995705269C181199279 @default.
- W1995705269 hasConceptScore W1995705269C185592680 @default.
- W1995705269 hasConceptScore W1995705269C2775896084 @default.
- W1995705269 hasConceptScore W1995705269C2777344606 @default.
- W1995705269 hasConceptScore W1995705269C2777495281 @default.
- W1995705269 hasConceptScore W1995705269C41183919 @default.
- W1995705269 hasConceptScore W1995705269C55493867 @default.
- W1995705269 hasConceptScore W1995705269C71240020 @default.
- W1995705269 hasConceptScore W1995705269C8010536 @default.
- W1995705269 hasIssue "18" @default.
- W1995705269 hasLocation W19957052691 @default.
- W1995705269 hasLocation W19957052692 @default.
- W1995705269 hasOpenAccess W1995705269 @default.
- W1995705269 hasPrimaryLocation W19957052691 @default.
- W1995705269 hasRelatedWork W1967703345 @default.
- W1995705269 hasRelatedWork W1980941430 @default.
- W1995705269 hasRelatedWork W1995705269 @default.
- W1995705269 hasRelatedWork W2005177356 @default.
- W1995705269 hasRelatedWork W2086755521 @default.
- W1995705269 hasRelatedWork W2143028632 @default.
- W1995705269 hasRelatedWork W2190127252 @default.
- W1995705269 hasRelatedWork W2319459235 @default.
- W1995705269 hasRelatedWork W2416308252 @default.
- W1995705269 hasRelatedWork W2808318619 @default.
- W1995705269 hasVolume "46" @default.
- W1995705269 isParatext "false" @default.
- W1995705269 isRetracted "false" @default.
- W1995705269 magId "1995705269" @default.
- W1995705269 workType "article" @default.