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- W1995745947 abstract "Summary Uptake of inosine and guanosine was measured in male germ ceils of the polychaete Nereis virens at different stages of development. In spermatogonia I (spg I) and spermatid stages, total inosine uptake at 12°C and ambient concentrations of 100 μmol/1 was relatively low (10–50 nmol·ml of packed cell volume pcv−l·h−1). A rapid increase (150–300 nmol·ml of pcv-1·h−1) was found during transition from spg I to spg II with a subsequent decline to low values (10–30 nmol·ml of pcv−1·h−1) in spermatocyte and spermatid stages. This transient increase may be related to the proliferative activity of spg I stages leading to spg II stages, which increases the demand of purine precursors for nucleic acid synthesis. Inosine uptake of spermatozoa stages was extremely variable, and high uptake rates (10–300 nmol·ml of pcv−1·h−1) were found in some, but not in all, cases. In all stages the uptake rates for guanosine remained at a lower level (below 75 nmol·ml of pcv−1·h−1), and no clear stage specific pattern was evident except for low values (<10 nmol·ml of pcv −1·h−1) in motile spermatozoa. For both nucleosides, two uptake components were identified: a linear component (measured at medium concentrations up to 300 μmol/l) and a saturable component (app. km: 9–60 μmol/l). The latter was inhibited by iodoacetamide suggesting the presence of an ATP-dependent facilitated transport mechanism. The presence of the two uptake components was related to the stage of gamete development: the saturable uptake component present in early spg I changed to a linear uptake in late spg I. In spg II stages, both uptake components were present while in spermatocytes, only the saturable component was present. Spermatids and early spermatozoa showed only a linear uptake. In the coelomic fluid, guanosine (0.5–20 μmol/l) and inosine (>3 μmol/l) were the main nucleosides; during spg II and spermatocyte stages, inosine reached high concentrations (100–1000 μmol/l). Key words: Nucleoside uptakeinosineguanosinegerm cellspolychaete Nereis virens" @default.
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- W1995745947 date "1997-01-01" @default.
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- W1995745947 title "Nucleoside uptake in male germ cells of the polychaete<i>Nereis virens</i>" @default.
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- W1995745947 doi "https://doi.org/10.1080/07924259.1997.9672566" @default.
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