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- W1995782268 abstract "In heat-resistance studies with spores of Clostridium sporogenes BC-2, an improved recovery medium was needed for severely heat-damaged spores as that used previously—Wynne medium in Miller-Prickett tubes—did not allow accurate counts of spores because of gas formation and disruption of agar. Initial tests with pour plates of Viande-Levure medium containing egg-yolk gave much increased counts for spores previously heated for 50 min at 112°C; this increase was attributed to the presence of egg-yolk. Addition of egg-yolk to Reinforced Clostridial Agar, All-Culture Medium and Trypticase Soy Agar showed that Trypticase Soy Agar with egg-yolk was the best recovery medium. For the final formulation, the value of supplementation with cysteine-HCl and methylene blue was also shown. The resultant Egg-yolk Trypticase Soy Agar is conveniently prepared from BBL Trypticase Soy Agar (40 g) with the addition of 0.4 g/l cysteine-HCl, 4 mg/1 of methylene blue and 2% Oxoid egg-yolk emulsion aseptically to the melted basal medium. For optimal spore counts, pour plates are incubated anaerobically for 5–7 d at 30°C." @default.
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- W1995782268 date "1983-10-01" @default.
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- W1995782268 title "Egg-yolk trypticase soy agar for the enumeration of heat-damaged spores of<i>Clostridium sporogenes</i>" @default.
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- W1995782268 doi "https://doi.org/10.1111/j.1365-2672.1983.tb01316.x" @default.
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