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- W1995847891 abstract "DNA from Aspergillus sp. has been reported not to contain 5-methylcytosine. However, it has been found that Aspergillus nidulans responds to 5-azacytidine, a drug that is a strong inhibitor of DNA methyltransferases. Therefore, we have re-examined the occurrence of 5-methylcytosine in DNA from Aspergillus flavus by using a highly sensitive and specific method for detection of modified bases in genomic DNA comprising high-performance liquid chromatography separation of nucleosides, labeling of the nucleoside with deoxynucleoside kinase and two-dimensional thin-layer chromatography. Our results show that 5-methylcytosine is present in DNA from A. flavus. We estimate the relative amounts of 5-methylcytosine to cytosine to be approximately 1/400." @default.
- W1995847891 created "2016-06-24" @default.
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- W1995847891 date "2001-11-01" @default.
- W1995847891 modified "2023-09-27" @default.
- W1995847891 title "DNA from<i>Aspergillus flavus</i>contains 5-methylcytosine" @default.
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- W1995847891 doi "https://doi.org/10.1111/j.1574-6968.2001.tb10939.x" @default.
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