FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1995880118> ?p ?o ?g. }

• W1995880118 endingPage "594" @default.
• W1995880118 startingPage "583" @default.
• W1995880118 abstract "Determination of the depth of membrane penetration provides important information for studies of membrane protein folding and protein–lipid interactions. Here, we use a combination of molecular dynamics (MD) simulations and depth-dependent fluorescence quenching to calibrate the methodology for extracting quantitative information on membrane penetration. In order to investigate the immersion depth of the fluorescent label in lipid bilayer, we studied 7-nitrobenz-2-oxa-1,3-diazole (NBD) attached to the lipid headgroup in NBD-PE incorporated into POPC bilayer. The immersion depth of NBD was estimated by measuring steady-state and time-resolved fluorescence quenching with spin-labeled lipids co-incorporated into lipid vesicles. Six different spin-labeled lipids were utilized: one with headgroup-attached Tempo probe (Tempo-PC) and five with acyl chain-labeled n-Doxyl moieties (n-Doxyl-PC where n is a chain labeling position equal to 5, 7, 10, 12, and 14, respectively). The Stern–Volmer analysis revealed that NBD quenching in membranes occurs by both static and dynamic collisional quenching processes. Using the methodology of Distribution Analysis, the immersion depth and the apparent half-width of the transversal distributions of the NBD moiety were estimated to be 14.7 and 6.7 Å, respectively, from the bilayer center. This position is independently validated by atomistic MD simulations of NBD-PE lipids in a POPC bilayer (14.4 Å). In addition, we demonstrate that MD simulations of the transverse overlap integrals between dye and quencher distributions can be used for proper analysis of the depth-dependent quenching profile. Finally, we illustrate the application of this methodology by determining membrane penetration of site selectively labeled mutants of diphtheria toxin T-domain." @default.
• W1995880118 created "2016-06-24" @default.
• W1995880118 creator A5025427559 @default.
• W1995880118 creator A5042930088 @default.
• W1995880118 creator A5048482508 @default.
• W1995880118 date "2014-08-09" @default.
• W1995880118 modified "2023-10-17" @default.
• W1995880118 title "Calibration of Distribution Analysis of the Depth of Membrane Penetration Using Simulations and Depth-Dependent Fluorescence Quenching" @default.
• W1995880118 cites W1513897103 @default.
• W1995880118 cites W1604232295 @default.
• W1995880118 cites W1966169046 @default.
• W1995880118 cites W1967875242 @default.
• W1995880118 cites W1969509994 @default.
• W1995880118 cites W1971121337 @default.
• W1995880118 cites W1971608828 @default.
• W1995880118 cites W1972519777 @default.
• W1995880118 cites W1976001472 @default.
• W1995880118 cites W1976224933 @default.
• W1995880118 cites W1983351621 @default.
• W1995880118 cites W1986516374 @default.
• W1995880118 cites W1989325271 @default.
• W1995880118 cites W1990515522 @default.
• W1995880118 cites W1994252543 @default.
• W1995880118 cites W2005122212 @default.
• W1995880118 cites W2007402446 @default.
• W1995880118 cites W2008822621 @default.
• W1995880118 cites W2015440087 @default.
• W1995880118 cites W2018531782 @default.
• W1995880118 cites W2029667189 @default.
• W1995880118 cites W2035266068 @default.
• W1995880118 cites W2049877637 @default.
• W1995880118 cites W2050917405 @default.
• W1995880118 cites W2052606098 @default.
• W1995880118 cites W2060362875 @default.
• W1995880118 cites W2067174909 @default.
• W1995880118 cites W2073071029 @default.
• W1995880118 cites W2073131401 @default.
• W1995880118 cites W2077356531 @default.
• W1995880118 cites W2078397595 @default.
• W1995880118 cites W2079390736 @default.
• W1995880118 cites W2081260116 @default.
• W1995880118 cites W2081431207 @default.
• W1995880118 cites W2083983859 @default.
• W1995880118 cites W2084514891 @default.
• W1995880118 cites W2085829586 @default.
• W1995880118 cites W2087528475 @default.
• W1995880118 cites W2089123087 @default.
• W1995880118 cites W2099559490 @default.
• W1995880118 cites W2114871137 @default.
• W1995880118 cites W2128572087 @default.
• W1995880118 cites W2138137879 @default.
• W1995880118 cites W2161984204 @default.
• W1995880118 cites W2171268876 @default.
• W1995880118 cites W223292669 @default.
• W1995880118 cites W2315359513 @default.
• W1995880118 cites W2324306253 @default.
• W1995880118 cites W2329340583 @default.
• W1995880118 cites W2950037181 @default.
• W1995880118 doi "https://doi.org/10.1007/s00232-014-9709-1" @default.
• W1995880118 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4321973" @default.
• W1995880118 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25107303" @default.
• W1995880118 hasPublicationYear "2014" @default.
• W1995880118 type Work @default.
• W1995880118 sameAs 1995880118 @default.
• W1995880118 citedByCount "30" @default.
• W1995880118 countsByYear W19958801182015 @default.
• W1995880118 countsByYear W19958801182016 @default.
• W1995880118 countsByYear W19958801182017 @default.
• W1995880118 countsByYear W19958801182018 @default.
• W1995880118 countsByYear W19958801182019 @default.
• W1995880118 countsByYear W19958801182020 @default.
• W1995880118 countsByYear W19958801182021 @default.
• W1995880118 countsByYear W19958801182022 @default.
• W1995880118 countsByYear W19958801182023 @default.
• W1995880118 crossrefType "journal-article" @default.
• W1995880118 hasAuthorship W1995880118A5025427559 @default.
• W1995880118 hasAuthorship W1995880118A5042930088 @default.
• W1995880118 hasAuthorship W1995880118A5048482508 @default.
• W1995880118 hasBestOaLocation W19958801182 @default.
• W1995880118 hasConcept C113196181 @default.
• W1995880118 hasConcept C120665830 @default.
• W1995880118 hasConcept C121332964 @default.
• W1995880118 hasConcept C121745418 @default.
• W1995880118 hasConcept C12554922 @default.
• W1995880118 hasConcept C127413603 @default.
• W1995880118 hasConcept C130316041 @default.
• W1995880118 hasConcept C147597530 @default.
• W1995880118 hasConcept C185154212 @default.
• W1995880118 hasConcept C185592680 @default.
• W1995880118 hasConcept C192157962 @default.
• W1995880118 hasConcept C2780943371 @default.
• W1995880118 hasConcept C39944091 @default.
• W1995880118 hasConcept C41625074 @default.
• W1995880118 hasConcept C42475967 @default.
• W1995880118 hasConcept C43617362 @default.
• W1995880118 hasConcept C55493867 @default.
• W1995880118 hasConcept C59593255 @default.
• W1995880118 hasConcept C80107235 @default.

• next