FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1995886605> ?p ?o ?g. }

• W1995886605 endingPage "2071" @default.
• W1995886605 startingPage "2060" @default.
• W1995886605 abstract "Abstract The Rv3377c gene from the Mycobacterium tuberculosis H37 genome is specifically limited to those Mycobacterium species that cause tuberculosis. We have demonstrated that the gene product of Rv3377c is a diterpene cyclase that catalyzes the formation of tuberculosinol from geranylgeranyl diphosphate (GGPP). However, the characteristics of this enzyme had not previously been studied in detail with homogeneously purified enzyme. The purified enzyme catalyzed the synthesis of tuberculosinyl diphosphate from GGPP, but it did not bring about the synthesis of tuberculosinol. Optimal conditions for the highest activity were found to be as follows: pH 7.5, 30 °C, Mg II (0.1 m M ), and Triton X‐100 (0.1 %). Under these conditions, the kinetic values of K M and k cat were determined to be 11.7±1.9 μ M for GGPP and 12.7±0.7 min −1 , respectively, whereas the specific activity was 186 nmol min −1 mg −1 . The enzyme activity was inhibited at substrate concentrations higher than 50 μ M . The catalytic activity was strongly inhibited by 15‐aza‐dihydrogeranylgeraniol and 5‐isopropyl‐ N , N , N ,2‐tetramethyl‐4‐(piperidine‐1‐carbonyloxy)benzenaminium chloride (Amo‐1618). The DXDTT 293–297 motif, corresponding to the DXDDTA motif conserved among terpene cyclases, was mutated in order to investigate its function. The middle D295 was found to be the most crucial entity for the catalysis. D293 and two threonine residues function synergistically to enhance the acidity of D295, possibly through hydrogen‐bonding networks. The Rv3377c enzyme could also react with (14 R / S )‐14,15‐oxidoGGPP to generate 3α‐ and 3β‐hydroxytuberculosinyl diphosphate. Conformational analyses were carried out with deuterium‐labeled GGPP and oxidoGGPP. We found that GGPP and (14 R )‐oxidoGGPP adopted a chair/chair conformation, but (14 S )‐oxidoGGPP adopted a boat/chair conformation. Interestingly, the conformations of oxidoGGPP for the A‐ring formation are the opposite of those of oxidosqualene when it is used as a substrate by squalene cyclases for the biosynthesis of hopene and tetrahymanol. (3 R )‐Oxidosqualene is folded in a boat conformation, whereas (3 S )‐2,3‐oxidosqualene folds into a chair conformation, for the formation of the A‐rings of the hopene and tetrahymanol skeletons, respectively." @default.
• W1995886605 created "2016-06-24" @default.
• W1995886605 creator A5008524509 @default.
• W1995886605 creator A5056901462 @default.
• W1995886605 date "2009-08-12" @default.
• W1995886605 modified "2023-10-10" @default.
• W1995886605 title "Characterization of the Rv3377c Gene Product, a Type‐B Diterpene Cyclase, from the <i>Mycobacterium tuberculosis</i> H37 Genome" @default.
• W1995886605 cites W1598954397 @default.
• W1995886605 cites W1599487951 @default.
• W1995886605 cites W1965552484 @default.
• W1995886605 cites W1972918540 @default.
• W1995886605 cites W1982132238 @default.
• W1995886605 cites W1994991258 @default.
• W1995886605 cites W1999960262 @default.
• W1995886605 cites W2002470296 @default.
• W1995886605 cites W2005923618 @default.
• W1995886605 cites W2012285919 @default.
• W1995886605 cites W2016453422 @default.
• W1995886605 cites W2017688884 @default.
• W1995886605 cites W2025667700 @default.
• W1995886605 cites W2027583333 @default.
• W1995886605 cites W2044466382 @default.
• W1995886605 cites W2047532982 @default.
• W1995886605 cites W2051456309 @default.
• W1995886605 cites W2053041342 @default.
• W1995886605 cites W2057577501 @default.
• W1995886605 cites W2066211687 @default.
• W1995886605 cites W2069123601 @default.
• W1995886605 cites W2070779989 @default.
• W1995886605 cites W2085881545 @default.
• W1995886605 cites W2087833823 @default.
• W1995886605 cites W2109297193 @default.
• W1995886605 cites W2110415727 @default.
• W1995886605 cites W2123126142 @default.
• W1995886605 cites W2142251083 @default.
• W1995886605 cites W2147171562 @default.
• W1995886605 cites W2147247168 @default.
• W1995886605 cites W2158023121 @default.
• W1995886605 cites W2158658436 @default.
• W1995886605 cites W2169259468 @default.
• W1995886605 cites W4301626766 @default.
• W1995886605 doi "https://doi.org/10.1002/cbic.200900248" @default.
• W1995886605 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19618417" @default.
• W1995886605 hasPublicationYear "2009" @default.
• W1995886605 type Work @default.
• W1995886605 sameAs 1995886605 @default.
• W1995886605 citedByCount "38" @default.
• W1995886605 countsByYear W19958866052012 @default.
• W1995886605 countsByYear W19958866052013 @default.
• W1995886605 countsByYear W19958866052014 @default.
• W1995886605 countsByYear W19958866052015 @default.
• W1995886605 countsByYear W19958866052016 @default.
• W1995886605 countsByYear W19958866052018 @default.
• W1995886605 countsByYear W19958866052019 @default.
• W1995886605 countsByYear W19958866052020 @default.
• W1995886605 countsByYear W19958866052021 @default.
• W1995886605 countsByYear W19958866052022 @default.
• W1995886605 countsByYear W19958866052023 @default.
• W1995886605 crossrefType "journal-article" @default.
• W1995886605 hasAuthorship W1995886605A5008524509 @default.
• W1995886605 hasAuthorship W1995886605A5056901462 @default.
• W1995886605 hasConcept C142724271 @default.
• W1995886605 hasConcept C181199279 @default.
• W1995886605 hasConcept C185592680 @default.
• W1995886605 hasConcept C2776710815 @default.
• W1995886605 hasConcept C2777975735 @default.
• W1995886605 hasConcept C2781069245 @default.
• W1995886605 hasConcept C55493867 @default.
• W1995886605 hasConcept C71240020 @default.
• W1995886605 hasConcept C71924100 @default.
• W1995886605 hasConceptScore W1995886605C142724271 @default.
• W1995886605 hasConceptScore W1995886605C181199279 @default.
• W1995886605 hasConceptScore W1995886605C185592680 @default.
• W1995886605 hasConceptScore W1995886605C2776710815 @default.
• W1995886605 hasConceptScore W1995886605C2777975735 @default.
• W1995886605 hasConceptScore W1995886605C2781069245 @default.
• W1995886605 hasConceptScore W1995886605C55493867 @default.
• W1995886605 hasConceptScore W1995886605C71240020 @default.
• W1995886605 hasConceptScore W1995886605C71924100 @default.
• W1995886605 hasIssue "12" @default.
• W1995886605 hasLocation W19958866051 @default.
• W1995886605 hasLocation W19958866052 @default.
• W1995886605 hasOpenAccess W1995886605 @default.
• W1995886605 hasPrimaryLocation W19958866051 @default.
• W1995886605 hasRelatedWork W1995954595 @default.
• W1995886605 hasRelatedWork W2014074068 @default.
• W1995886605 hasRelatedWork W2037535229 @default.
• W1995886605 hasRelatedWork W2065283623 @default.
• W1995886605 hasRelatedWork W2071451411 @default.
• W1995886605 hasRelatedWork W2421969030 @default.
• W1995886605 hasRelatedWork W3082072074 @default.
• W1995886605 hasRelatedWork W4309047958 @default.
• W1995886605 hasRelatedWork W93398777 @default.
• W1995886605 hasRelatedWork W1984318426 @default.
• W1995886605 hasVolume "10" @default.
• W1995886605 isParatext "false" @default.
• W1995886605 isRetracted "false" @default.
• W1995886605 magId "1995886605" @default.

• next