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- W1995957029 abstract "We report a set of baculovirus transfer vectors for parallel expression of proteins in fusion with a panel of affinity tags including GST, protein A, thioredoxin, CBP, and FLAG. This suite includes vectors to generate recombinant baculovirus by homologous recombination in insect cells or using the Bac-to-Bac technology. An application of the vector suite approach to the vitamin D receptor (VDR), a protein mainly expressed as inclusion bodies in Escherichia coli, is presented. We found that expression in fusion with GST and protein A provided an efficient compromise of excellent purification with acceptable yields and costs." @default.
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- W1995957029 date "2009-02-01" @default.
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- W1995957029 title "A set of baculovirus transfer vectors for screening of affinity tags and parallel expression strategies" @default.
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- W1995957029 doi "https://doi.org/10.1016/j.ab.2008.10.044" @default.
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