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• W1996063838 abstract "Herpesvirus transmission is sporadic, and infection may be asymptomatic or present only with secondary lesions after dissemination. Consequently host entry remains ill-understood. Experimental infections can be informative, but depend on inoculations that are inherently artificial and so need validation. Mice are a widely used experimental host. Alert mice inhale readily small (5 μl) liquid volumes, and Indian ink, luciferase or radiolabel delivered thus distributed to the nasopharynx and oropharynx. Murid Herpesvirus-4 or Herpes simplex virus type 1 delivered thus infected only the nose, arguing that host entry is nasal rather than oral. Marker or virus delivery to the lung depended on general anesthesia and a large inoculum volume (30 μl), and so needs further validation of physiological relevance. While lungs could be infected at lower doses than the upper respiratory tract, tracking experiments showed that nasal inocula pass mostly into the oropharynx, even when restricted to 1 μl. Thus, the relative inefficiency of experimental upper respiratory tract infection was attributable to limited liquid retention in this site. Nonetheless low volume intranasal delivery to alert mice provides a convenient way to model experimentally an apparently natural mode of herpesvirus host entry." @default.
• W1996063838 created "2016-06-24" @default.
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• W1996063838 creator A5060955442 @default.
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• W1996063838 date "2014-09-01" @default.
• W1996063838 modified "2023-10-14" @default.
• W1996063838 title "Herpesvirus delivery to the murine respiratory tract" @default.
• W1996063838 cites W1974275338 @default.
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• W1996063838 doi "https://doi.org/10.1016/j.jviromet.2014.06.003" @default.
• W1996063838 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24928692" @default.
• W1996063838 hasPublicationYear "2014" @default.
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