FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1996072926> ?p ?o ?g. }

• W1996072926 endingPage "3442" @default.
• W1996072926 startingPage "3433" @default.
• W1996072926 abstract "Polyether ionophores are a unique class of polyketides with broad-spectrum activity and outstanding potency for the control of drug-resistant bacteria and parasites, and they are produced exclusively by actinomycetes. A special epoxidase gene encoding a critical tailoring enzyme involved in the biosynthesis of these compounds has been found in all five of the complete gene clusters of polyether ionophores published so far. To detect potential producer strains of these antibiotics, a pair of degenerate primers was designed according to the conserved regions of the five known polyether epoxidases. A total of 44 putative polyether epoxidase gene-positive strains were obtained by the PCR-based screening of 1,068 actinomycetes isolated from eight different habitats and 236 reference strains encompassing eight major families of Actinomycetales. The isolates spanned a wide taxonomic diversity based on 16S rRNA gene analysis, and actinomycetes isolated from acidic soils seemed to be a promising source of polyether ionophores. Four genera were detected to contain putative polyether epoxidases, including Micromonospora, which has not previously been reported to produce polyether ionophores. The designed primers also detected putative epoxidase genes from diverse known producer strains that produce polyether ionophores unrelated to the five published gene clusters. Moreover, phylogenetic and chemical analyses showed a strong correlation between the sequence of polyether epoxidases and the structure of encoded polyethers. Thirteen positive isolates were proven to be polyether ionophore producers as expected, and two new analogues were found. These results demonstrate the feasibility of using this epoxidase gene screening strategy to aid the rapid identification of known products and the discovery of unknown polyethers in actinomycetes." @default.
• W1996072926 created "2016-06-24" @default.
• W1996072926 creator A5001796193 @default.
• W1996072926 creator A5013728344 @default.
• W1996072926 creator A5022773264 @default.
• W1996072926 creator A5049015126 @default.
• W1996072926 creator A5054075486 @default.
• W1996072926 creator A5061687627 @default.
• W1996072926 date "2011-05-15" @default.
• W1996072926 modified "2023-10-16" @default.
• W1996072926 title "Genetic Screening Strategy for Rapid Access to Polyether Ionophore Producers and Products in Actinomycetes" @default.
• W1996072926 cites W1977739164 @default.
• W1996072926 cites W1980301840 @default.
• W1996072926 cites W1986038186 @default.
• W1996072926 cites W1991349770 @default.
• W1996072926 cites W1996390229 @default.
• W1996072926 cites W1998251886 @default.
• W1996072926 cites W2003706451 @default.
• W1996072926 cites W2009156161 @default.
• W1996072926 cites W2016605307 @default.
• W1996072926 cites W2019081530 @default.
• W1996072926 cites W2025121964 @default.
• W1996072926 cites W2026974870 @default.
• W1996072926 cites W2028286172 @default.
• W1996072926 cites W2032746165 @default.
• W1996072926 cites W2033578880 @default.
• W1996072926 cites W2036360388 @default.
• W1996072926 cites W2036561818 @default.
• W1996072926 cites W2058499983 @default.
• W1996072926 cites W2064157736 @default.
• W1996072926 cites W2078415854 @default.
• W1996072926 cites W2085248040 @default.
• W1996072926 cites W2086479630 @default.
• W1996072926 cites W2088084221 @default.
• W1996072926 cites W2090764881 @default.
• W1996072926 cites W2091729115 @default.
• W1996072926 cites W2101365358 @default.
• W1996072926 cites W2123781174 @default.
• W1996072926 cites W2125121305 @default.
• W1996072926 cites W2137015675 @default.
• W1996072926 cites W2142925821 @default.
• W1996072926 cites W2149779144 @default.
• W1996072926 cites W2152114458 @default.
• W1996072926 cites W2155190875 @default.
• W1996072926 cites W2167109158 @default.
• W1996072926 cites W2324486379 @default.
• W1996072926 cites W2325639093 @default.
• W1996072926 cites W4235229030 @default.
• W1996072926 doi "https://doi.org/10.1128/aem.02915-10" @default.
• W1996072926 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3126441" @default.
• W1996072926 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21421776" @default.
• W1996072926 hasPublicationYear "2011" @default.
• W1996072926 type Work @default.
• W1996072926 sameAs 1996072926 @default.
• W1996072926 citedByCount "17" @default.
• W1996072926 countsByYear W19960729262012 @default.
• W1996072926 countsByYear W19960729262013 @default.
• W1996072926 countsByYear W19960729262014 @default.
• W1996072926 countsByYear W19960729262015 @default.
• W1996072926 countsByYear W19960729262016 @default.
• W1996072926 countsByYear W19960729262019 @default.
• W1996072926 countsByYear W19960729262020 @default.
• W1996072926 countsByYear W19960729262021 @default.
• W1996072926 countsByYear W19960729262022 @default.
• W1996072926 countsByYear W19960729262023 @default.
• W1996072926 crossrefType "journal-article" @default.
• W1996072926 hasAuthorship W1996072926A5001796193 @default.
• W1996072926 hasAuthorship W1996072926A5013728344 @default.
• W1996072926 hasAuthorship W1996072926A5022773264 @default.
• W1996072926 hasAuthorship W1996072926A5049015126 @default.
• W1996072926 hasAuthorship W1996072926A5054075486 @default.
• W1996072926 hasAuthorship W1996072926A5061687627 @default.
• W1996072926 hasBestOaLocation W19960729262 @default.
• W1996072926 hasConcept C104317684 @default.
• W1996072926 hasConcept C193252679 @default.
• W1996072926 hasConcept C2776060363 @default.
• W1996072926 hasConcept C2779920670 @default.
• W1996072926 hasConcept C2781382891 @default.
• W1996072926 hasConcept C42062724 @default.
• W1996072926 hasConcept C523546767 @default.
• W1996072926 hasConcept C54355233 @default.
• W1996072926 hasConcept C55493867 @default.
• W1996072926 hasConcept C86803240 @default.
• W1996072926 hasConcept C89423630 @default.
• W1996072926 hasConceptScore W1996072926C104317684 @default.
• W1996072926 hasConceptScore W1996072926C193252679 @default.
• W1996072926 hasConceptScore W1996072926C2776060363 @default.
• W1996072926 hasConceptScore W1996072926C2779920670 @default.
• W1996072926 hasConceptScore W1996072926C2781382891 @default.
• W1996072926 hasConceptScore W1996072926C42062724 @default.
• W1996072926 hasConceptScore W1996072926C523546767 @default.
• W1996072926 hasConceptScore W1996072926C54355233 @default.
• W1996072926 hasConceptScore W1996072926C55493867 @default.
• W1996072926 hasConceptScore W1996072926C86803240 @default.
• W1996072926 hasConceptScore W1996072926C89423630 @default.
• W1996072926 hasIssue "10" @default.
• W1996072926 hasLocation W19960729261 @default.
• W1996072926 hasLocation W19960729262 @default.

• next