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- W1996087154 abstract "Integrated studies that address proteins structure and function in the new era of systems biology and genomics often require the application of high-throughput approaches for parallel production of many different purified proteins from the same organism. Cytochromes c—electron transfer proteins carrying one or more hemes covalently bound to the polypeptide chain—are essential in most organisms. However, they are one of the most recalcitrant classes of proteins with respect to heterologous expression because post-translational incorporation of hemes is required for proper folding and stability. We have addressed this challenge by designing two families of vectors (total of 6 vectors) suitable for ligation-independent cloning and developing a pipeline for expression and solubility analysis of cytochromes c. This system has been validated by expression analysis of thirty genes from Shewanella oneidensis coding for cytochromes c or cytochromes c-type domains predicted to have 1–4 hemes. Out of 30 targets, 26 (87%) were obtained in soluble form in one or more vectors. This work establishes a methodology for high-throughput expression of this class of proteins and provides a clone resource for the microbiological and functional genomics research communities." @default.
- W1996087154 created "2016-06-24" @default.
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- W1996087154 date "2008-11-01" @default.
- W1996087154 modified "2023-09-26" @default.
- W1996087154 title "Addressing Shewanella oneidensis “cytochromome”: The first step towards high-throughput expression of cytochromes c" @default.
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- W1996087154 doi "https://doi.org/10.1016/j.pep.2008.06.014" @default.
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