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- W1996091285 abstract "Detection of the 15N nucleus in studies of the metabolism of branched-chain amino acids was carried out by recording the 1H nuclear magnetic resonance (NMR) spectrum through the effect of the 15N-1H coupling. The Selective Excitation Unit performed a 90° selective proton pulse to overcome the strong water signal and baseline distorsion. In order to obtain quantitative measurement, the leucine β protons and the valine (internal reference) β protons coupled to 15N nucleus were simultaneously detected. This NMR method was tested on muscle homogenate incubated with [15N] leucine (≈3 μmoles/g). The supernatant was directly observed by NMR. The sensitivity of this indirect method was found to be far higher than direct observation of the 15N signals by 15N NMR." @default.
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- W1996091285 date "1989-05-01" @default.
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- W1996091285 title "Heteronuclear 15N/1H spin echo NMR: An in vitro quantitative analysis of leucine transamination in rat skeletal muscle" @default.
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- W1996091285 doi "https://doi.org/10.1016/s0006-291x(89)80106-8" @default.
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