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• W1996097788 abstract "Poly(glycidyl methacrylate-co-ethylene dimethacrylate) monoliths have been synthetized in fused-silica capillary. The monomer mixture composition, initiation mode and porogen composition were optimized in order to provide a monolith with an homogeneous morphology and able to generate an electroosmotic flow via the incorporation of a small percentage of monomers possessing sulfonate group. Anti-ochratoxin A antibodies were immobilized through a single step on the epoxy groups leading to a miniaturized immunoextraction column. In order to evaluate the specificity of the analyte-antigen interaction on this immunosorbent, the retention of ochratoxin A was examined on this support but also on two complementary sorbents: one constituted by the non-bonded monolith and another one bonded with non-specific antibodies. Only the monolith bonded with anti-ochratoxin A antibodies lead to retention, showing the specificity of the interactions involved. This affinity phase based on a monolithic polymer support exhibits a high potential for specific preconcentration of small molecules." @default.
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• W1996097788 date "2007-05-01" @default.
• W1996097788 modified "2023-09-25" @default.
• W1996097788 title "Optimization of in-situ monolithic synthesis for immunopreconcentration in capillary" @default.
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• W1996097788 doi "https://doi.org/10.1016/j.chroma.2007.03.031" @default.
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