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- W1996152381 abstract "Bovine corneal tissue was employed in the study of variable factors in a method of deep-freezing, storage and thawing, using Trypane blue staining as indicator of cellular damage. Variations in cell population density in the endothelial sheet were studied as a prerequisite for the evaluation of damage during the successive stages of the freezing procedures. 90% of the cells in the endothelial sheet survived freezing to ––160°C when the corneal buttons were passed through increasing concentrations of DMSO in serum and frozen at a certain rate in 1 ml 8% DMSO. The final concentration of DMSO, the amount of fluid surrounding the buttons during freezing and the duration of the isotermic part of the freezing curve were found to be critical." @default.
- W1996152381 created "2016-06-24" @default.
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- W1996152381 date "1974-01-01" @default.
- W1996152381 modified "2023-09-24" @default.
- W1996152381 title "Corneal Cryopreservation Evaluated by Trypane Blue Staining" @default.
- W1996152381 doi "https://doi.org/10.1159/000264597" @default.
- W1996152381 hasPublicationYear "1974" @default.
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