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- W1996209562 abstract "The non-structural protein NS-1 of porcine parvovirus (PPV) could be a useful antigen for differentiating pigs infected with PPV from those vaccinated with inactivated whole virus. NS-1 was expressed in Escherichia coli using the pET-32a (+) vector. The fusion protein, which was expressed at a high level, was similar antigenically to the native NS-1 protein as determined by Western blot assay using polyclonal antibodies from pigs vaccinated with alive PPV vaccine. A simple procedure was used to purify the fusion protein. This research lays the foundation for using the NS-1 protein for clinical diagnosis of pigs infected with PPV vs. those vaccinated with inactivated whole virus vaccine." @default.
- W1996209562 created "2016-06-24" @default.
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- W1996209562 date "2009-04-01" @default.
- W1996209562 modified "2023-09-25" @default.
- W1996209562 title "Expression, purification, and characterization of recombinant NS-1, the porcine parvovirus non-structural protein" @default.
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- W1996209562 doi "https://doi.org/10.1016/j.jviromet.2008.11.016" @default.
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