Matches in SemOpenAlex for { <https://semopenalex.org/work/W1996283586> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W1996283586 endingPage "204" @default.
- W1996283586 startingPage "191" @default.
- W1996283586 abstract "The relation between rate of protein synthesis and intracellular pH (pHi) was investigated in the eggs of the sea urchin Strongylocentrotus purpuratus. Increasing external pH (pHo) resulted in raising pHi of eggs and also in increased rate of protein synthesis. Similarly, at constant pHo, adding various concentrations of NH4Cl to eggs caused graded increases of both pHi and protein synthesis. Using various concentrations of NH4Cl at a low pHo and incubating eggs at high pHo, we compared protein synthesis under similar pHi conditions and this revealed that at least half the increased protein synthesis stimulated by NH4Cl is independent of induced rise of pHi, as also seems to be chromosome condensation which was never observed in eggs incubated at high pHos. The additional pH-independent event triggered by NH4Cl does not appear related to elevated free Ca2+, since protein synthesis and chromosome condensation do not require external Ca2+ and no increases of free Ca2+ sufficient to activate the Ca2+-calmodulin-mediated enzyme NAD kinase occurred. Monensin disrupts intravesicular pH gradients but does not stimulate protein synthesis, indicating that this local effect, also promoted by NH4Cl, is not involved in ammonia-induced increase of protein synthesis. Using two other amines which have low pKa values, benzocaine and tricaine, we observed 2-fold increases in protein synthesis rates, even though pHi was lowered. While the exact nature of the pH-independent event(s) triggered by NH4Cl, and possibly by other amines, remains unidentified, its possible involvement in normal mitosis is stressed." @default.
- W1996283586 created "2016-06-24" @default.
- W1996283586 creator A5006626877 @default.
- W1996283586 creator A5046761651 @default.
- W1996283586 date "1986-01-01" @default.
- W1996283586 modified "2023-10-15" @default.
- W1996283586 title "The relation between intracellular pH and rate of protein synthesis in sea urchin eggs and the existence of a pH-independent event triggered by ammonia" @default.
- W1996283586 cites W1495429371 @default.
- W1996283586 cites W1775749144 @default.
- W1996283586 cites W1969201204 @default.
- W1996283586 cites W1969451595 @default.
- W1996283586 cites W1974896117 @default.
- W1996283586 cites W1975565947 @default.
- W1996283586 cites W1981341970 @default.
- W1996283586 cites W1985182356 @default.
- W1996283586 cites W1985854049 @default.
- W1996283586 cites W1995824339 @default.
- W1996283586 cites W2004991062 @default.
- W1996283586 cites W2015564029 @default.
- W1996283586 cites W2021843494 @default.
- W1996283586 cites W2023918299 @default.
- W1996283586 cites W2029466947 @default.
- W1996283586 cites W2030339035 @default.
- W1996283586 cites W2031704718 @default.
- W1996283586 cites W2045231736 @default.
- W1996283586 cites W2046239497 @default.
- W1996283586 cites W2046277481 @default.
- W1996283586 cites W2050098727 @default.
- W1996283586 cites W2050927844 @default.
- W1996283586 cites W2053766825 @default.
- W1996283586 cites W2056768252 @default.
- W1996283586 cites W2058442820 @default.
- W1996283586 cites W2059813738 @default.
- W1996283586 cites W2064646563 @default.
- W1996283586 cites W2064750590 @default.
- W1996283586 cites W2067119870 @default.
- W1996283586 cites W2076624549 @default.
- W1996283586 cites W2077080379 @default.
- W1996283586 cites W2095625177 @default.
- W1996283586 cites W2158714866 @default.
- W1996283586 cites W2409072825 @default.
- W1996283586 cites W4238605098 @default.
- W1996283586 doi "https://doi.org/10.1016/0014-4827(86)90438-6" @default.
- W1996283586 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3940228" @default.
- W1996283586 hasPublicationYear "1986" @default.
- W1996283586 type Work @default.
- W1996283586 sameAs 1996283586 @default.
- W1996283586 citedByCount "52" @default.
- W1996283586 countsByYear W19962835862012 @default.
- W1996283586 countsByYear W19962835862015 @default.
- W1996283586 countsByYear W19962835862016 @default.
- W1996283586 countsByYear W19962835862022 @default.
- W1996283586 crossrefType "journal-article" @default.
- W1996283586 hasAuthorship W1996283586A5006626877 @default.
- W1996283586 hasAuthorship W1996283586A5046761651 @default.
- W1996283586 hasConcept C12554922 @default.
- W1996283586 hasConcept C2776908312 @default.
- W1996283586 hasConcept C2777569064 @default.
- W1996283586 hasConcept C2780224938 @default.
- W1996283586 hasConcept C3675279 @default.
- W1996283586 hasConcept C55493867 @default.
- W1996283586 hasConcept C86803240 @default.
- W1996283586 hasConcept C95444343 @default.
- W1996283586 hasConceptScore W1996283586C12554922 @default.
- W1996283586 hasConceptScore W1996283586C2776908312 @default.
- W1996283586 hasConceptScore W1996283586C2777569064 @default.
- W1996283586 hasConceptScore W1996283586C2780224938 @default.
- W1996283586 hasConceptScore W1996283586C3675279 @default.
- W1996283586 hasConceptScore W1996283586C55493867 @default.
- W1996283586 hasConceptScore W1996283586C86803240 @default.
- W1996283586 hasConceptScore W1996283586C95444343 @default.
- W1996283586 hasIssue "1" @default.
- W1996283586 hasLocation W19962835861 @default.
- W1996283586 hasLocation W19962835862 @default.
- W1996283586 hasOpenAccess W1996283586 @default.
- W1996283586 hasPrimaryLocation W19962835861 @default.
- W1996283586 hasRelatedWork W1486965885 @default.
- W1996283586 hasRelatedWork W1965263924 @default.
- W1996283586 hasRelatedWork W1991055100 @default.
- W1996283586 hasRelatedWork W2017238034 @default.
- W1996283586 hasRelatedWork W2095382894 @default.
- W1996283586 hasRelatedWork W2095566763 @default.
- W1996283586 hasRelatedWork W2332218218 @default.
- W1996283586 hasRelatedWork W2410285078 @default.
- W1996283586 hasRelatedWork W2433802694 @default.
- W1996283586 hasRelatedWork W2472448991 @default.
- W1996283586 hasVolume "162" @default.
- W1996283586 isParatext "false" @default.
- W1996283586 isRetracted "false" @default.
- W1996283586 magId "1996283586" @default.
- W1996283586 workType "article" @default.