Matches in SemOpenAlex for { <https://semopenalex.org/work/W1996295557> ?p ?o ?g. }
Showing items 1 to 63 of
63
with 100 items per page.
- W1996295557 endingPage "290" @default.
- W1996295557 startingPage "282" @default.
- W1996295557 abstract "The spectrophotometric determination of protein in millimicrogram quantities was described which is suitable for analysis of microtome sections of tissue and other samples of histochemical interest. The macro procedure of Greif was adapted to the micro scale and a modification was employed in which protein was quantitatively precipitated with an excess of bromsulfalein at a low pH and the excess dye was photometrically determined after addition of alkali. Kjeldahl nitrogen analysis of the protein was used for standardization and the results were expressed in terms of protein-nitrogen. With samples and equipment described, a standard error of 1.4% corresponding to 0.9 mµg. of protein-nitrogen was observed. With smaller pipettes and the same cuvettes the volumes employed could be rather easily reduced to about one half with a doubling of the sensitivity of the method. Further increase in sensitivity could be achieved without inconvenience by the use of cuvettes of smaller diameter, e.g. 0.7-0.8 mm., and pipettes of correspondingly reduced capacities." @default.
- W1996295557 created "2016-06-24" @default.
- W1996295557 creator A5031097025 @default.
- W1996295557 creator A5060157106 @default.
- W1996295557 date "1954-07-01" @default.
- W1996295557 modified "2023-09-27" @default.
- W1996295557 title "STUDIES IN HISTOCHEMISTRY XXXI. A METHOD FOR THE DETERMINATION OF PROTEIN IN MILLIMICROGRAM QUANTITIES" @default.
- W1996295557 doi "https://doi.org/10.1177/2.4.282" @default.
- W1996295557 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/13174791" @default.
- W1996295557 hasPublicationYear "1954" @default.
- W1996295557 type Work @default.
- W1996295557 sameAs 1996295557 @default.
- W1996295557 citedByCount "84" @default.
- W1996295557 crossrefType "journal-article" @default.
- W1996295557 hasAuthorship W1996295557A5031097025 @default.
- W1996295557 hasAuthorship W1996295557A5060157106 @default.
- W1996295557 hasBestOaLocation W19962955571 @default.
- W1996295557 hasConcept C113196181 @default.
- W1996295557 hasConcept C120665830 @default.
- W1996295557 hasConcept C121332964 @default.
- W1996295557 hasConcept C147789679 @default.
- W1996295557 hasConcept C151552171 @default.
- W1996295557 hasConcept C169478075 @default.
- W1996295557 hasConcept C178790620 @default.
- W1996295557 hasConcept C185592680 @default.
- W1996295557 hasConcept C37160838 @default.
- W1996295557 hasConcept C43617362 @default.
- W1996295557 hasConcept C537208039 @default.
- W1996295557 hasConcept C680638 @default.
- W1996295557 hasConceptScore W1996295557C113196181 @default.
- W1996295557 hasConceptScore W1996295557C120665830 @default.
- W1996295557 hasConceptScore W1996295557C121332964 @default.
- W1996295557 hasConceptScore W1996295557C147789679 @default.
- W1996295557 hasConceptScore W1996295557C151552171 @default.
- W1996295557 hasConceptScore W1996295557C169478075 @default.
- W1996295557 hasConceptScore W1996295557C178790620 @default.
- W1996295557 hasConceptScore W1996295557C185592680 @default.
- W1996295557 hasConceptScore W1996295557C37160838 @default.
- W1996295557 hasConceptScore W1996295557C43617362 @default.
- W1996295557 hasConceptScore W1996295557C537208039 @default.
- W1996295557 hasConceptScore W1996295557C680638 @default.
- W1996295557 hasIssue "4" @default.
- W1996295557 hasLocation W19962955571 @default.
- W1996295557 hasLocation W19962955572 @default.
- W1996295557 hasOpenAccess W1996295557 @default.
- W1996295557 hasPrimaryLocation W19962955571 @default.
- W1996295557 hasRelatedWork W1530682216 @default.
- W1996295557 hasRelatedWork W154224372 @default.
- W1996295557 hasRelatedWork W1996295557 @default.
- W1996295557 hasRelatedWork W2057357993 @default.
- W1996295557 hasRelatedWork W2081578590 @default.
- W1996295557 hasRelatedWork W2157505332 @default.
- W1996295557 hasRelatedWork W2275885462 @default.
- W1996295557 hasRelatedWork W2338788857 @default.
- W1996295557 hasRelatedWork W2942801853 @default.
- W1996295557 hasRelatedWork W779592659 @default.
- W1996295557 hasVolume "2" @default.
- W1996295557 isParatext "false" @default.
- W1996295557 isRetracted "false" @default.
- W1996295557 magId "1996295557" @default.
- W1996295557 workType "article" @default.