FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1996304552> ?p ?o ?g. }

• W1996304552 endingPage "4556" @default.
• W1996304552 startingPage "4550" @default.
• W1996304552 abstract "Organic chemistry has made possible the synthesis of molecules that expand on Nature's genetic alphabet. Using the previously described nonstandard DNA base pair constructed from isoguanine and 5-methylisocytosine, we report a highly specific and sensitive method that allows for the fast and specific quantitation of genetic sequences in a closed tube format. During PCR amplification, enzymatic site-specific incorporation of a quencher covalently linked to isoguanine allows for the simultaneous detection and identification of multiple targets. The specificity of method is then established by analysis of thermal denaturation or melting of the amplicons. The appropriate functions of all reactions are further verified by incorporation of an independent target into the reaction mixture. We report that the method is sensitive down to the single copy level, and specificity is demonstrated by multiplexed end-point genotypic analysis of four targets simultaneously using four separate fluorescent reporters. The method is general enough for quantitative and qualitative analysis of both RNA and DNA using previously developed primer sets. Though the method described employs the commonly used PCR, the enzymatic incorporation of reporter groups into DNA site-specifically should find broad utility throughout molecular biology." @default.
• W1996304552 created "2016-06-24" @default.
• W1996304552 creator A5002148776 @default.
• W1996304552 creator A5028324522 @default.
• W1996304552 creator A5033620285 @default.
• W1996304552 creator A5053305968 @default.
• W1996304552 creator A5056490752 @default.
• W1996304552 creator A5075097036 @default.
• W1996304552 date "2004-03-20" @default.
• W1996304552 modified "2023-09-25" @default.
• W1996304552 title "Nucleic Acid Analysis Using an Expanded Genetic Alphabet to Quench Fluorescence" @default.
• W1996304552 cites W1559760142 @default.
• W1996304552 cites W1942367992 @default.
• W1996304552 cites W1970663985 @default.
• W1996304552 cites W1990734020 @default.
• W1996304552 cites W1991198187 @default.
• W1996304552 cites W1994091239 @default.
• W1996304552 cites W2026710381 @default.
• W1996304552 cites W2027098970 @default.
• W1996304552 cites W2046395875 @default.
• W1996304552 cites W2063629547 @default.
• W1996304552 cites W2065164352 @default.
• W1996304552 cites W2070721758 @default.
• W1996304552 cites W2100404753 @default.
• W1996304552 cites W2113959415 @default.
• W1996304552 cites W2139880603 @default.
• W1996304552 cites W2152049155 @default.
• W1996304552 cites W2156021383 @default.
• W1996304552 cites W2949150794 @default.
• W1996304552 doi "https://doi.org/10.1021/ja0315558" @default.
• W1996304552 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15070373" @default.
• W1996304552 hasPublicationYear "2004" @default.
• W1996304552 type Work @default.
• W1996304552 sameAs 1996304552 @default.
• W1996304552 citedByCount "88" @default.
• W1996304552 countsByYear W19963045522012 @default.
• W1996304552 countsByYear W19963045522013 @default.
• W1996304552 countsByYear W19963045522014 @default.
• W1996304552 countsByYear W19963045522015 @default.
• W1996304552 countsByYear W19963045522016 @default.
• W1996304552 countsByYear W19963045522017 @default.
• W1996304552 countsByYear W19963045522018 @default.
• W1996304552 countsByYear W19963045522019 @default.
• W1996304552 countsByYear W19963045522020 @default.
• W1996304552 countsByYear W19963045522021 @default.
• W1996304552 countsByYear W19963045522023 @default.
• W1996304552 crossrefType "journal-article" @default.
• W1996304552 hasAuthorship W1996304552A5002148776 @default.
• W1996304552 hasAuthorship W1996304552A5028324522 @default.
• W1996304552 hasAuthorship W1996304552A5033620285 @default.
• W1996304552 hasAuthorship W1996304552A5053305968 @default.
• W1996304552 hasAuthorship W1996304552A5056490752 @default.
• W1996304552 hasAuthorship W1996304552A5075097036 @default.
• W1996304552 hasConcept C104317684 @default.
• W1996304552 hasConcept C121332964 @default.
• W1996304552 hasConcept C129312508 @default.
• W1996304552 hasConcept C153911025 @default.
• W1996304552 hasConcept C178790620 @default.
• W1996304552 hasConcept C185581394 @default.
• W1996304552 hasConcept C185592680 @default.
• W1996304552 hasConcept C21951064 @default.
• W1996304552 hasConcept C24107716 @default.
• W1996304552 hasConcept C2777563447 @default.
• W1996304552 hasConcept C3017666073 @default.
• W1996304552 hasConcept C49105822 @default.
• W1996304552 hasConcept C52001828 @default.
• W1996304552 hasConcept C54871578 @default.
• W1996304552 hasConcept C552990157 @default.
• W1996304552 hasConcept C55493867 @default.
• W1996304552 hasConcept C62520636 @default.
• W1996304552 hasConcept C70721500 @default.
• W1996304552 hasConcept C8185291 @default.
• W1996304552 hasConcept C86803240 @default.
• W1996304552 hasConcept C90583042 @default.
• W1996304552 hasConcept C91881484 @default.
• W1996304552 hasConceptScore W1996304552C104317684 @default.
• W1996304552 hasConceptScore W1996304552C121332964 @default.
• W1996304552 hasConceptScore W1996304552C129312508 @default.
• W1996304552 hasConceptScore W1996304552C153911025 @default.
• W1996304552 hasConceptScore W1996304552C178790620 @default.
• W1996304552 hasConceptScore W1996304552C185581394 @default.
• W1996304552 hasConceptScore W1996304552C185592680 @default.
• W1996304552 hasConceptScore W1996304552C21951064 @default.
• W1996304552 hasConceptScore W1996304552C24107716 @default.
• W1996304552 hasConceptScore W1996304552C2777563447 @default.
• W1996304552 hasConceptScore W1996304552C3017666073 @default.
• W1996304552 hasConceptScore W1996304552C49105822 @default.
• W1996304552 hasConceptScore W1996304552C52001828 @default.
• W1996304552 hasConceptScore W1996304552C54871578 @default.
• W1996304552 hasConceptScore W1996304552C552990157 @default.
• W1996304552 hasConceptScore W1996304552C55493867 @default.
• W1996304552 hasConceptScore W1996304552C62520636 @default.
• W1996304552 hasConceptScore W1996304552C70721500 @default.
• W1996304552 hasConceptScore W1996304552C8185291 @default.
• W1996304552 hasConceptScore W1996304552C86803240 @default.
• W1996304552 hasConceptScore W1996304552C90583042 @default.
• W1996304552 hasConceptScore W1996304552C91881484 @default.
• W1996304552 hasIssue "14" @default.

• next