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• W1996319732 abstract "B cell antigen receptors (BCR) are composed of an antigen binding subunit, the membrane Ig, and Ig-α/Ig-β heterodimers, that contain a transducing motif named ITAM for “immuno-receptor tyrosine-based activation motif.” Ig-α and Ig-β ITAMs only differ by four amino acids located before the second conserved tyrosine (DCSM in Ig-α and QTAT in Ig-β), which determine the in vitro association of Ig-α with the src kinase fyn. We have previously shown that Ig-α and Ig-β BCR subunits activate different signaling pathways by expressing, in B cells, FcγRII chimeras containing the cytoplasmic tails of Ig-α or Ig-β. We report here that the signaling capacity of Ig-β ITAM is regulated by peptide sequences located inside (QTAT region) or outside the ITAM (flanking sequences). Furthermore, when isolated, Ig-α and Ig-β ITAM have similar abilities as the entire Ig-α tail and the whole BCR in triggering tyrosine kinase activation, an increase of intracellular calcium concentration as well as late events of cell activation as assessed by cytokine secretion. These data show that alterations that modify the ability of Ig-α and Ig-β to interact in vitro with the src kinase fyn (switch between QTAT and DCSM) also determine signal transduction capabilities of these molecules expressed in B cells. B cell antigen receptors (BCR) are composed of an antigen binding subunit, the membrane Ig, and Ig-α/Ig-β heterodimers, that contain a transducing motif named ITAM for “immuno-receptor tyrosine-based activation motif.” Ig-α and Ig-β ITAMs only differ by four amino acids located before the second conserved tyrosine (DCSM in Ig-α and QTAT in Ig-β), which determine the in vitro association of Ig-α with the src kinase fyn. We have previously shown that Ig-α and Ig-β BCR subunits activate different signaling pathways by expressing, in B cells, FcγRII chimeras containing the cytoplasmic tails of Ig-α or Ig-β. We report here that the signaling capacity of Ig-β ITAM is regulated by peptide sequences located inside (QTAT region) or outside the ITAM (flanking sequences). Furthermore, when isolated, Ig-α and Ig-β ITAM have similar abilities as the entire Ig-α tail and the whole BCR in triggering tyrosine kinase activation, an increase of intracellular calcium concentration as well as late events of cell activation as assessed by cytokine secretion. These data show that alterations that modify the ability of Ig-α and Ig-β to interact in vitro with the src kinase fyn (switch between QTAT and DCSM) also determine signal transduction capabilities of these molecules expressed in B cells. INTRODUCTIONThe transducing capacities of BCR 1The abbreviations used are: BCRB cell antigen receptor(s)mIgmembrane immunoglobulinIL-2interleukin-2FcRFcγRIImmotif. are based on its multimolecular structure. BCRs are composed of antigen binding units, the membrane immunoglobulins (mIg), noncovalently associated with transducing subunits, the Ig-α/Ig-β heterodimers. The cytoplasmic tails of these associated chains become phosphorylated after cross-linking of mIg (1Gold M.R. Matsuuchi L. Kelly R.B. DeFranco A.L. Proc. Natl. Acad. Sci. U. S. A. 1991; 88: 3436-3440Google Scholar) and associate with intracellular effectors (2Campbell K.S. Cambier J.C. EMBO J. 1990; 9: 441-448Google Scholar) including the src kinases lyn, fyn, blk, lck (3Yamanashi Y. Kakiuchi T. Mizuguchi J. Yamamoto T. Toyoshima K. Science. 1991; 251: 192-194Google Scholar, 4Burkhardt A.L. Brunswick M. Bolen J.B. Mond J.J. Proc. Natl. Acad. Sci. U. S. A. 1991; 88: 7410-7414Google Scholar, 5Lin J. Justement L.B. J. Immunol. 1992; 149: 1548-1555Google Scholar, 6Campbell M.A. Sefton B.M. Mol. Cell. Biol. 1992; 12: 2315-2321Google Scholar), and the src-related kinase syk (7Hutchcroft J.E. Harrison M.L. Geahlen R.L. J. Biol. Chem. 1992; 267: 8613-8619Google Scholar, 8Yamada T. Taniguchi T. Yang C. Yasue S. Saito H. Yamamura H. Eur. J. Biochem. 1993; 213: 455-459Google Scholar), as well as other kinases such as PI-3 kinase and unidentified phosphoproteins (9Clark M.R. Campbell K.S. Kazlauskas A. Johnson S.A. Hertz M. Potter T.A. Pleiman C. Cambier J.C. Science. 1992; 258: 123-126Google Scholar). By adsorbing B cell lysates on fusion proteins containing the cytoplasmic domains of Ig-α or Ig-β, the unphosphorylated cytoplasmic domains were shown to bind to different kinases. The cytoplasmic tail of Ig-α interact with fyn and lyn and with an unidentified molecule of 38 kDa, whereas the cytoplasmic tail of Ig-β binds to two unidentified phosphoproteins of 40 and 42 kDa (9Clark M.R. Campbell K.S. Kazlauskas A. Johnson S.A. Hertz M. Potter T.A. Pleiman C. Cambier J.C. Science. 1992; 258: 123-126Google Scholar). The activation of tyrosine kinase is followed by an increase of intracellular calcium concentration (10Baixeras E. Kroemer G. Cuende E. Marquez C. Bosca L. Martinez J.E.A. Martinez A.-C. Immunol. Rev. 1993; 132: 5-47Google Scholar). Typical cytoplasmic calcium increase includes an initial release of calcium from intracellular stores followed by an influx of extracellular calcium, which is involved in lymphocytes activation (11Gelfand E.W. Cheung R.K. Mills G.B. Grinstein S. Eur. J. Immunol. 1988; 18: 917-922Google Scholar, 12Dennis G.J. Mizuguchi J. McMillan V. Finkelman F.D. Ohara J. Mond J.J. J. Immunol. 1987; 138: 4307-4312Google Scholar). However, both cytoplasmic domains of Ig-α and Ig-β, like associated subunits of T cell antigen receptors or Fc receptors, contain an ITAM (immunoreceptor tyrosine-based activation motif), which contains conserved tyrosine and leucine or isoleucine amino acids (YXX(L/I)XXXXXXXYXX(L/I)) (13Reth M. Nature. 1989; 338: 383-384Google Scholar). One particularity of Ig-α and Ig-β ITAMs is their high homology because they mostly differ by the four amino acids located before the second conserved tyrosine, the same four residues determining the in vitro association of Ig-α with fyn (14Clark M.R. Johnson S.A. Cambier J.C. EMBO J. 1994; 13: 1911-1919Google Scholar).Functional analysis of Ig-α and Ig-β cytoplasmic domains in B cells established that both are able to induce an increase of intracellular calcium concentration (15Sanchez M. Misulovin Z. Burkhardt A.L. Mahajan S. Costa T. Franke R. Bolen J.B. Nussenzweig M. J. Exp. Med. 1993; 178: 1049-1055Google Scholar, 16Kim K.M. Alber G. Weiser P. Reth M. Eur. J. Immunol. 1993; 23: 911-916Google Scholar, 17Taddie J.A. Hurley T.R. Hardwick B.S. Sefton B.M. J. Biol. Chem. 1994; 269: 13529-13535Google Scholar) with qualitative differences (18Choquet D. Ku G. Cassard S. Malissen B. Korn H. Fridman W.H. Bonnerot C. J. Biol. Chem. 1994; 269: 6491-6497Google Scholar). Although Ig-β was as efficient as Ig-α in triggering protein-tyrosine kinase activation, only Ig-α-containing chimeras were able to trigger an efficient signal transduction leading to an extracellular calcium influx and interleukin-2 (IL-2) production in the IIA1.6 B cell line. Ig-β triggered an oscillatory release from intracellular calcium stores and no IL-2 secretion (18Choquet D. Ku G. Cassard S. Malissen B. Korn H. Fridman W.H. Bonnerot C. J. Biol. Chem. 1994; 269: 6491-6497Google Scholar). Ig-α and Ig-β cytoplasmic domains therefore possess their own distinct signaling capabilities. In this study, the molecular basis of the different signaling capacities of Ig-α and Ig-β cytoplasmic tails were analyzed. We showed that BCR subunits transducing activities are based on the ITAM conserved sequence, but they may be regulated by unconserved sequences located inside or outside the motif.RESULTSThe intracellular signaling activity of mIg-associated subunits, Ig-α and Ig-β, was analyzed by expressing, in the B cell line IIA1.6, chimeras fusing the cytoplasmic domain of either Ig-α or Ig-β to the extracellular and transmembrane domains of FcγRII. Both chimeras activated tyrosine kinases (Fig. 1a), but only Ig-α chimeras (c.Ig-α) stimulation induced intracellular calcium modifications composed of an initial release from intracellular stores followed by an extracellular calcium influx, whereas Ig-β chimeras (c.Ig-β) stimulation triggered an oscillatory release of calcium from intracellular stores (Fig. 1b) (18Choquet D. Ku G. Cassard S. Malissen B. Korn H. Fridman W.H. Bonnerot C. J. Biol. Chem. 1994; 269: 6491-6497Google Scholar). Interestingly, only Ig-α chimeras, as well as endogenous mIg, were efficient to induce the secretion of cytokines.The role of calcium influx in B cell signaling was analyzed by measuring IL-2 secretion after stimulation of Ig-α chimera or mIgG in conditions that prevented the extracellular calcium influx. Cells expressing c·Ig-α were incubated with EGTA to chelate the extracellular calcium or in a calcium free medium. Cross-linking of c.Ig-α or of mIgG in the 1 mM EGTA-containing medium (or in calcium-free medium) prevented calcium influx (data not shown) and IL-2 secretion by transfected cells (Fig. 1c). Both signaling events were restored by adding 1 mM CaCl2. Extracellular calcium influx is therefore required for the triggering of cytokine secretion after cross-linking of Ig-α chimera or the whole BCR. Moreover, although the cytoplasmic tail of Ig-α and Ig-β both contain an ITAM, only Ig-α induced efficient signal transduction. Ig-α therefore accounts for the ability of BCR to induce calcium influx and subsequent cell activation events. Peptide sequence of Ig-α and Ig-β ITAM must determine their interactions with specific cytoplasmic effectors that induce either calcium influx and cytokine secretion or calcium oscillatory release.Requirements of the Conserved ITAM Tyrosine Residues for the Signaling Capacities of Ig-α and Ig-β TailsTo identify the peptide sequences of Ig-α and Ig-β cytoplasmic tails involved in the induction of calcium influx, a mutational analysis was performed by using FcR-based chimeras (see Table I). The cytoplasmic tails of Ig-α and Ig-β were shown to interact with different tyrosine kinases, and the phosphorylation of conserved tyrosine residues constituting their ITAM enhanced these interactions. To evaluate in our model the role of the tyrosine residues present in the ITAM, they were individually mutated to alanine in the cytoplasmic domain of Ig-α (c.Ig-α A23 and c.Ig-α A34) or mutated together in the Ig-β tail (c.Ig-β A15,A26). These constructions were stably expressed in IIA1.6 cells, and surface expressions were evaluated by flow cytometry using indirect immunofluorescence (Fig. 2).Table IChimeric constructs of FcR type II and cytoplasmic domains of Ig-α and Ig-βFcR-Ig-α/β chimeras and mutantsCytoplasmic domains23 34↓↓c.Ig-αRKRWQNEKFGVDMPDDYEDENLYEGLNLDDCSMYEDISRGLQGTYQDVGNLHIGDAQLEKPc.Ig-α A23RKRWQNEKFGVDMPDDYEDENLAEGLNLDDCSMYEDISRGLQGTYQDVGNLHIGDAQLEKPc.Ig-α A34RKRWQNEKFGVDMPDDYEDENLYEGLNLDDCSMAEDISRGLQGTYQDVGNLHIGDAQLEKPc.Ig-α QTATRKRWQNEKFGVDMPDDYEDENLYEGLNLDQTATYEDISRGLQGTYQDVGNLHIGDAQLEKPc.Ig-α mRKR——Δ——-EDENLYEGLNLDDCSMYEDISRGLQGT——–Δ——–15 26↓↓c.Ig-βRKRDGKAGM________EEDHTYEGLNIDQTATYEDIVTLRTGEVKWSVGEHPGQEc.Ig-β A15,A26RKRDGKAGM________EEDHTAEGLNIDQTATAEDIVTLRTGEVKWSVGEHPGQEc.Ig-β DCSMRKRDGKAGM________EEDHTYEGLNIDDCSMYEDIVTLRTGEVKWSVGEHPGQEc.Ig-β mRKR–Δ—________EEDHTYEGLNIDQTATYEDIVTLRTGE—–Δ—— Open table in a new tab Fig. 2Surface expression of Ig-α and Ig-β chimeras in IIA1.6 transfected cells by indirect fluorescence. Cells were incubated with the anti-FcR antibody 24G2 and then with the fluorescein-conjugated mouse anti-rat IgG antiserum (white histograms). The dark histograms represent the control experiments where the cells were only incubated with the fluorescein-coupled antibodies.View Large Image Figure ViewerDownload (PPT)The earliest known BCR signaling event is tyrosine kinase activation leading to a cascade of intracellular protein phosphorylations. Our previous results showed that the cytoplasmic domains of Ig-α and Ig-β are able to trigger phosphorylation of similar major intracellular proteins (Fig. 1a). As expected, c.Ig-α A23 and c.Ig-α A34 or c.Ig-β A15,A26 were inefficient to trigger tyrosine phosphorylation of intracellular proteins, whereas in control experiments, stimulation of mIg triggered tyrosine phosphorylation in all the transfectant cells (Fig. 3a). The stimulation of these mutated chimeras was also inefficient to induce any changes of the intracellular calcium concentration, as measured at the single cell level by video imaging (Fig. 3b), and they did not trigger IL-2 secretion (Fig. 3c). All the transfected cells were responsive to stimulation via mIg. Ig-α and Ig-β cytoplasmic tails trigger two different signaling pathways, which are therefore both dependent on ITAM tyrosine residues. As shown by others (15Sanchez M. Misulovin Z. Burkhardt A.L. Mahajan S. Costa T. Franke R. Bolen J.B. Nussenzweig M. J. Exp. Med. 1993; 178: 1049-1055Google Scholar, 19Flaswinkel H. Reth M. EMBO J. 1994; 13: 83-89Google Scholar, 20Burkhardt A.L. Costa T. Misulovin Z. Stealy B. Bolen J.B. Nussenzweig M. Mol. Cell. Biol. 1994; 14: 1095-1103Google Scholar, 21Williams G.T. Peaker C.J.G. Patel K.J. Neuberger M.S. Proc. Natl. Acad. Sci. U. S. A. 1994; 91: 474-478Google Scholar), the conserved tyrosines residues are required for the induction of transmembrane signaling through Ig-α and Ig-β. Because both chains activate different signaling pathways, some Ig-α or Ig-β specific amino acids must modulate their signaling activities.Fig. 3The mutation of the tyrosine residues in the entire cytoplasmic tail of Ig-α (c.Ig-α A23 and c.Ig-α A34) or Ig-β (c.Ig-β A15,A26) prevent the triggering of intracellular signaling events. Chimeras were cross-linked in the same conditions as before. a, tyrosine phosphorylations of intracellular proteins after cross-linking of chimeras (anti-chimeras) or endogenous mIg (anti-IgG). b, measurement of intracellular Ca2+ concentrations at the single cell level after cross-linking of mutant chimeras (arithmetic averages). The arrows indicate the triggering of stimulation. The dotted arrows indicate the control-stimulation experiments where the endogenous mIg were cross-linked. Boxed curves represent examples of a single cell response. c, IL-2 secretion by the cells expressing chimeras. Chimeras were cross-linked as before (anti-chimera), and in the control experiments, mIg were cross-linked with 15 μg/ml of F(ab)′2 fragments of rabbit anti-mouse IgG (anti-IgG). The results presented here are the means of three experiments done in duplicate and are normalized on the mIg responses, which represent the maximum of IL-2 secretion.View Large Image Figure ViewerDownload (PPT)Four Amino Acids in the ITAM Determine the Signaling Capabilities of Ig-α and Ig-β Cytoplasmic DomainsOne major difference between Ig-α and Ig-β ITAM consists of four amino acids preceding the second tyrosine of the motifs. The peptide sequence DCSM is present in Ig-α, whereas Ig-β contains the sequence QTAT. This difference has been related to the specific binding of src family kinases to the cytoplasmic tails of Ig-α or Ig-β. In a nonphosphorylated status, only the molecules bearing the four amino acids DCSM bind the tyrosine kinase fyn (14Clark M.R. Johnson S.A. Cambier J.C. EMBO J. 1994; 13: 1911-1919Google Scholar). The role of these four amino acids in the signaling activity of Ig-α and Ig-β was investigated by expressing two chimeras containing a switch of the sequences QTAT versus DCSM in Ig-α and Ig-β cytoplasmic tails (c.Ig-α QTAT and c.Ig-β DCSM) (see Table I and Fig. 2). The cross-linking of c.Ig-β DCSM triggered the phosphorylation of numerous intracellular proteins and an extracellular calcium influx leading to IL-2 secretion (Fig. 4, a, b, and c). The exchange of QTAT by DCSM inside the entire Ig-β cytoplasmic domain was therefore sufficient to convert the transducing phenotype of Ig-β in that of Ig-α tail. Because the cross-linking of c.Ig-β was not able to trigger a calcium influx, the capacity of c.Ig-α and c.Ig-β DCSM to trigger a calcium influx is probably determined by the presence of the DCSM polypeptide sequence in their cytoplasmic tail. In contrast, the conversion of the DCSM sequence into QTAT in the Ig-α tail did not prevent the triggering of calcium influx and tyrosine kinase activation (Fig. 4, a, b, and c). In all these experiments, the transfectant cells were responsive to anti-IgG. The results obtained with the switching mutants showed that the ability of Ig-β to trigger different intracellular events may be modulated by amino acids located between the conserved ITAM residues, whereas the similar switch (QTAT) in the entire Ig-α cytoplasmic tail does not affect its signaling capacity. The conformation induced by the peptide sequences surrounding Ig-β ITAM must be important to induce its interactions with specific intracellular effectors.Fig. 4The inversion of the peptide sequences DCSM and QTAT change the transducing capacities of Ig-α and Ig-β cytoplasmic domains. a, tyrosine kinase activation after cross-linking of the two chimeras containing the inversion (c.Ig-β DCSM and c.Ig-α QTAT) in the same conditions than before. b, intracellular calcium measurement after cross-linking of the chimeras. As before, the arrows show the addition of the second antibody to cross-link the chimeras, and the dotted arrows indicate the addition of F(ab)′2 fragments of rabbit anti-mouse IgG. Boxed curves show examples of a single cell response. c, IL-2 secretion after cross-linking of the chimeras or after cross-linking of mIg for control experiments. The measurements were done in the same conditions as in Fig. 3.View Large Image Figure ViewerDownload (PPT)The Activity of Ig-β ITAM Is Regulated by Flanking SequencesThe sequences flanking the two motifs are different between Ig-α and Ig-β cytoplasmic tails. To test whether flanking sequences affect the signaling capacities of ITAMs, chimeras containing isolated Ig-α or Ig-β motifs (c.Ig-α m and c.Ig-β m; Table I) were expressed in IIA1.6 cells (Fig. 2). The stimulation of c.Ig-α m induced tyrosine phosphorylation of several intracellular substrates similar to those induced by the cross-linking of c.Ig-α, with a maximum of phosphorylation intensity after 1 min of stimulation (Fig. 5a). The pattern of calcium signaling obtained after cross-linking of c.Ig-α m was similar to the pattern obtained after stimulation of c.Ig-α, comprising a release of calcium from the intracellular stores and an influx of extracellular calcium (Fig. 5b). This calcium response triggered by the cross-linking of c.Ig-α m was followed by later events of signals transduction, as measured by IL-2 secretion (Fig. 5c). Thus, when isolated, the Ig-α ITAM was as efficient as the entire Ig-α cytoplasmic tail and as the whole BCR in triggering cytokine production. The ITAM of Ig-α is therefore fully functional inside the entire Ig-α tail environment, and it is not regulated directly by its flanking sequences.Fig. 5Ig-α ITAM functions as the entire cytoplasmic tail of Ig-α, whereas the motif of Ig-β does not function as the entire intracellular domain of Ig-β. a, intracellular protein phosphorylation induced by the cross-linking of the chimeras for the indicated times. The cells were also stimulated by their endogenous mIgG (arrow). b, measurement of intracellular calcium concentration after cross-linking of chimeras containing the Ig-α or Ig-β ITAM (c.Ig-α m and c.Ig-β m, respectively). The arrows indicate the triggering of the stimulation, and the boxed curves represent an example of a single cell response. c, stimulation of both chimeras triggered IL-2 secretion. The cells all produced IL-2 after cross-linking of mIgG. This experiment was done under the same conditions as described in the legend to Fig. 3, Fig. 4.View Large Image Figure ViewerDownload (PPT)Surprisingly, the isolated Ig-β ITAM had different signaling abilities than the entire cytoplasmic tail of Ig-β. In contrast to intracellular calcium oscillations induced by Ig-β chimeras, the cross-linking of c.Ig-β m triggered a complete calcium response composed of an initial calcium release from intracellular stores followed by an extracellular calcium influx (Fig. 5b), like the cross-linking of mIg, c.Ig-α, or c.Ig-α m. Moreover, the stimulation of c.Ig-β m was very efficient in inducing IL-2 secretion (Fig. 5c). In contrast, no clear differences of phosphoproteins were detected after cross-linking of c.Ig-β m or c.Ig-β, whereas the maximum intensities of the phosphorylations were respectively observed after 2 and 1 min of cross-linking (Figs. 1a and 5a). Thus, the isolated Ig-β ITAM and the entire cytoplasmic tail of Ig-β are both able to activate tyrosine kinases but differ in terms of calcium signaling and triggering of IL-2 secretion.These results show that the two isolated Ig-α and Ig-β ITAMs are able to trigger the same intracellular events leading to cytokine production and that the unconserved environment of an ITAM can regulate the signaling activity of this kind of tyrosine-based activating motifs.DISCUSSIONStimulation of BCR triggers intracellular events such as protein kinase activation and increase of intracellular calcium concentration resulting in cell activation. In the present work, we evaluated the relative contributions of different domains of Ig-α and Ig-β cytoplasmic tails in B cell signaling. The cytoplasmic domain of Ig-α, and more specifically its ITAM, reflects the transducing capacities of the whole BCR in terms of phosphoproteins induction, calcium mobilization, and IL-2 secretion, which is dependent on the triggering of a calcium influx. In contrast, Ig-β ITAM activity is modulated by amino acids located between the conserved residues and by the ITAM flanking sequences, although the signaling capacities of both ITAMs require conserved tyrosine residues.The transducing events triggered by Ig-α ITAM are similar to those triggered by the entire intracellular domain of Ig-α and by the whole BCR. In this cascade of intracellular signaling events, the triggering of extracellular calcium influx is a crucial step of B cell activation, which may be inhibited by the cross-linking of mIg with FcR for IgG (22Amigorena S. Bonnerot C. Drake J.R. Choquet D. Hunziker W. Guillet J.-G. Webster P. Sautes C. Mellman I. Fridman W.H. Science. 1992; 256: 1808-1812Google Scholar, 23Choquet D. Partiseti M. Amigorena S. Bonnerot C. Fridman W.H. Korn H. J. Cell. Biol. 1993; 121: 355-363Google Scholar, 24Diegel M.L. Rankin B.M. Bolen J.B. Dubois P.M. Kiener P.A. J. Biol. Chem. 1994; 269: 11409-11416Google Scholar). In addition, chelation of extracellular calcium with EGTA inhibits the triggering of both calcium influx and lymphokine secretion after stimulation of either BCR or Ig-α chimeras (Fig. 1c). Furthermore, Ig-β chimera stimulation did not efficiently induce lymphokine secretion, although this molecule triggered intracellular tyrosine phosphorylation and oscillatory releases of intracellular calcium stores without extracellular influx (18Choquet D. Ku G. Cassard S. Malissen B. Korn H. Fridman W.H. Bonnerot C. J. Biol. Chem. 1994; 269: 6491-6497Google Scholar). It has been shown that in T cells, IgM-Ig-β and CD8-Ig-β chimeras were able to trigger IL-2 after cross-linking (20Burkhardt A.L. Costa T. Misulovin Z. Stealy B. Bolen J.B. Nussenzweig M. Mol. Cell. Biol. 1994; 14: 1095-1103Google Scholar, 17Taddie J.A. Hurley T.R. Hardwick B.S. Sefton B.M. J. Biol. Chem. 1994; 269: 13529-13535Google Scholar), but T cells could lack an intracellular effector that regulates Ig-β activity in B cells. The triggering of late B cell activation events as assessed by lymphokine secretion is therefore based on the signaling capacities of Ig-α cytoplasmic domain, which may be reduced to its ITAM.The Ig-α and Ig-β cytoplasmic tails therefore triggered distinct intracellular events, whereas, strikingly, the isolated ITAMs have the same efficient transducing capacities. Stimulation of both chimeras containing the isolated motifs induced the phosphorylation of the same intracellular substrates and triggered both calcium influx and IL-2 secretion. These results are consistent with data obtained with similar μCD8-based chimeras containing the isolated ITAM of Ig-α or Ig-β (25Law D.A. Chan V.W.F. Datta S.K. DeFranco A.L. Curr. Biol. 1993; 3: 645-657Google Scholar). They showed that cross-linking of these chimeras triggered the same protein tyrosine phosphorylations and calcium mobilization and induced the interaction of the motifs with the same kinases lyn, fyn, and syk. Another study has also shown that phosphorylated ITAMs of Ig-α or Ig-β have similar abilities to interact with fyn in vitro (14Clark M.R. Johnson S.A. Cambier J.C. EMBO J. 1994; 13: 1911-1919Google Scholar). The phosphorylation of the ITAM tyrosine therefore seems to be a crucial step to induce the transducing cascade because the mutation of ITAM-conserved tyrosine residues totally abolished the signaling capabilities of both Ig-α and Ig-β cytoplasmic tails (Fig. 3; 15Sanchez M. Misulovin Z. Burkhardt A.L. Mahajan S. Costa T. Franke R. Bolen J.B. Nussenzweig M. J. Exp. Med. 1993; 178: 1049-1055Google Scholar and 19Flaswinkel H. Reth M. EMBO J. 1994; 13: 83-89Google Scholar, 20Burkhardt A.L. Costa T. Misulovin Z. Stealy B. Bolen J.B. Nussenzweig M. Mol. Cell. Biol. 1994; 14: 1095-1103Google Scholar, 21Williams G.T. Peaker C.J.G. Patel K.J. Neuberger M.S. Proc. Natl. Acad. Sci. U. S. A. 1994; 91: 474-478Google Scholar), perhaps by preventing the interaction with fyn or with other kinase(s). Therefore, as already described, the ITAM-tyrosine residues are required to give to Ig-α and Ig-β their transducing capabilities.The specificity of Ig-α and Ig-β tails to trigger different signaling events seems determined by nonconserved sequences between the cytoplasmic tails of Ig-α and Ig-β. One of the differences between the two ITAM-amino acid sequences is constituted of four amino acids located between the conserved tyrosine residues (DCSM in Ig-α and QTAT in Ig-β). Our results show that this difference of four amino acids do not seem to play a role in the transduction events triggered by the isolated motifs (Fig. 5), but it is important when the motifs are examined in their entire cytoplasmic environment. Indeed, the conversion of the four amino acids QTAT into DCSM in the Ig-β cytoplasmic tail changed the signaling activity of Ig-β into those of Ig-α (Fig. 4), perhaps by enhancing the basal level of fyn associated with the chimera, because only the DCSM-bearing ITAMs were able to interact with fyn in vitro (14Clark M.R. Johnson S.A. Cambier J.C. EMBO J. 1994; 13: 1911-1919Google Scholar). In contrast, when the amino acids DCSM were changed into QTAT in Ig-α cytoplasmic tail, the transducing capacities of Ig-α were not switched, showing that QTAT is necessary but not sufficient to inactivate the calcium influx and/or to activate calcium oscillations. These results indicate that there are other amino acids inside or outside the Ig-β ITAM that also regulate its signaling capacities.The ITAM of Ig-β, in contrast to the entire cytoplasmic domain of Ig-β, is fully efficient to trigger intracellular events leading to lymphokine secretion. This indicates that ITAM flanking sequences regulate the activity of the Ig-β ITAM. These sequences are inefficient, however, when the four amino acids QTAT are replaced by DCSM (Fig. 4). This suggests that other amino acids that differ between the two motifs, like the four amino acids located before the first tyrosine of the two motifs (DENL in Ig-α and EDHT in Ig-β), may play a role in the regulation capacity of flanking regions of Ig-β ITAM. This sequence could regulate the Ig-β ITAM by interacting with intracellular proteins, like the two unidentified p40 and p42 phosphoproteins (9Clark M.R. Campbell K.S. Kazlauskas A. Johnson S.A. Hertz M. Potter T.A. Pleiman C. Cambier J.C. Science. 1992; 258: 123-126Google Scholar), which bind specifically to Ig-β. Two nonexclusive models may be proposed to account for the specificity of Ig-α and Ig-β intracellular signaling. First, the nonconserved sequences located inside or outside the ITAM induce conformational modifications, which modulate the affinity of cytoplasmic effectors for conserved tyrosine residues located in the ITAM. Second, each domain of the cytoplasmic tails of Ig-α and Ig-β (QTAT, DCSM, or flanking sequences) may interact with distinct intracellular effectors, which are specifically recruited by receptor aggregation and thus determine the activation of different intracellular signaling pathways. However, the fact that the deletion of one of the flanking sequences is enough to allow Ig-β to trigger IL-2 secretion after stimulation (data not shown) supports a conformational role for the flanking sequences.The signaling activity of the BCR-associated subunits can be regulated by cross-linking of mIg with FcR (22Amigorena S. Bonnerot C. Drake J.R. Choquet D. Hunziker W. Guillet J.-G. Webster P. Sautes C. Mellman I. Fridman W.H. Science. 1992; 256: 1808-1812Google Scholar, 26Muta T. Kurosaki T. Misulovin Z. Sanchez M. Nussenzweig C. Ravetch J.V. Nature. 1994; 368: 70-73Google Scholar) involving the phosphatase PTP1C, which acts as a trans-regulator of the Ig-α/Ig-β ITAM activity (27D'Ambrosio D. Hippen K.H. Minskoff S.A. Mellman I. Pani G. Siminovitch K.A. Cambier J.C. Science. 1995; 268: 293-297Google Scholar). Our results show that QTAT or flanking sequences regulate Ig-β ITAM signaling activities, meaning that these activating motifs can be regulated in a cis-position. It remains to establish what is the function of these sequences in the whole BCR complex and whether cytoplasmic ligands of Ig-β cytoplasmic tail may regulate the signaling activity carry out by Ig-α cytoplasmic tail, as is suggested by results showing that mIg stimulation of spleen B cells could trigger calcium oscillations (28Wilson H.A. Greenblatt D. Poenie M. Finkelman F.D. Tsien R. J. Exp. Med. 1987; 166: 601-606Google Scholar). During B cell activation and differentiation, BCR composition may vary because heterodimers with cytoplasmic deleted isoforms of Ig-α or Ig-β have been reported (29Friedrich R.J. Campbell K.S. Cambier J.C. J. Immunol. 1993; 150: 2814-2822Google Scholar, 30Ishihara K. Wood Jr., W.J. Wall R. Sakaguchi N. Michnoff C. Tucker P.W. Kincade P.W. J. Immunol. 1993; 150: 2253-2262Google Scholar). The heterodimers that contain one of these deleted forms therefore probably have the signaling capacities of either Ig-α or Ig-β. Variations in cytosol composition could also determine the activation of one chain more than the other or could trigger different events depending on the effectors present in the cells, like for Ig-β chimeras in T cells that are able to induce IL-2 secretion (17Taddie J.A. Hurley T.R. Hardwick B.S. Sefton B.M. J. Biol. Chem. 1994; 269: 13529-13535Google Scholar, 20Burkhardt A.L. Costa T. Misulovin Z. Stealy B. Bolen J.B. Nussenzweig M. Mol. Cell. Biol. 1994; 14: 1095-1103Google Scholar). The analysis of relative signaling capacities of Ig-α and Ig-β and their peptide sequence requirement is a first step in understanding BCR signaling events during the different stages of B cell differentiation or activation. INTRODUCTIONThe transducing capacities of BCR 1The abbreviations used are: BCRB cell antigen receptor(s)mIgmembrane immunoglobulinIL-2interleukin-2FcRFcγRIImmotif. are based on its multimolecular structure. BCRs are composed of antigen binding units, the membrane immunoglobulins (mIg), noncovalently associated with transducing subunits, the Ig-α/Ig-β heterodimers. The cytoplasmic tails of these associated chains become phosphorylated after cross-linking of mIg (1Gold M.R. Matsuuchi L. Kelly R.B. DeFranco A.L. Proc. Natl. Acad. Sci. U. S. A. 1991; 88: 3436-3440Google Scholar) and associate with intracellular effectors (2Campbell K.S. Cambier J.C. EMBO J. 1990; 9: 441-448Google Scholar) including the src kinases lyn, fyn, blk, lck (3Yamanashi Y. Kakiuchi T. Mizuguchi J. Yamamoto T. Toyoshima K. Science. 1991; 251: 192-194Google Scholar, 4Burkhardt A.L. Brunswick M. Bolen J.B. Mond J.J. Proc. Natl. Acad. Sci. U. S. A. 1991; 88: 7410-7414Google Scholar, 5Lin J. Justement L.B. J. Immunol. 1992; 149: 1548-1555Google Scholar, 6Campbell M.A. Sefton B.M. Mol. Cell. Biol. 1992; 12: 2315-2321Google Scholar), and the src-related kinase syk (7Hutchcroft J.E. Harrison M.L. Geahlen R.L. J. Biol. Chem. 1992; 267: 8613-8619Google Scholar, 8Yamada T. Taniguchi T. Yang C. Yasue S. Saito H. Yamamura H. Eur. J. Biochem. 1993; 213: 455-459Google Scholar), as well as other kinases such as PI-3 kinase and unidentified phosphoproteins (9Clark M.R. Campbell K.S. Kazlauskas A. Johnson S.A. Hertz M. Potter T.A. Pleiman C. Cambier J.C. Science. 1992; 258: 123-126Google Scholar). By adsorbing B cell lysates on fusion proteins containing the cytoplasmic domains of Ig-α or Ig-β, the unphosphorylated cytoplasmic domains were shown to bind to different kinases. The cytoplasmic tail of Ig-α interact with fyn and lyn and with an unidentified molecule of 38 kDa, whereas the cytoplasmic tail of Ig-β binds to two unidentified phosphoproteins of 40 and 42 kDa (9Clark M.R. Campbell K.S. Kazlauskas A. Johnson S.A. Hertz M. Potter T.A. Pleiman C. Cambier J.C. Science. 1992; 258: 123-126Google Scholar). The activation of tyrosine kinase is followed by an increase of intracellular calcium concentration (10Baixeras E. Kroemer G. Cuende E. Marquez C. Bosca L. Martinez J.E.A. Martinez A.-C. Immunol. Rev. 1993; 132: 5-47Google Scholar). Typical cytoplasmic calcium increase includes an initial release of calcium from intracellular stores followed by an influx of extracellular calcium, which is involved in lymphocytes activation (11Gelfand E.W. Cheung R.K. Mills G.B. Grinstein S. Eur. J. Immunol. 1988; 18: 917-922Google Scholar, 12Dennis G.J. Mizuguchi J. McMillan V. Finkelman F.D. Ohara J. Mond J.J. J. Immunol. 1987; 138: 4307-4312Google Scholar). However, both cytoplasmic domains of Ig-α and Ig-β, like associated subunits of T cell antigen receptors or Fc receptors, contain an ITAM (immunoreceptor tyrosine-based activation motif), which contains conserved tyrosine and leucine or isoleucine amino acids (YXX(L/I)XXXXXXXYXX(L/I)) (13Reth M. Nature. 1989; 338: 383-384Google Scholar). One particularity of Ig-α and Ig-β ITAMs is their high homology because they mostly differ by the four amino acids located before the second conserved tyrosine, the same four residues determining the in vitro association of Ig-α with fyn (14Clark M.R. Johnson S.A. Cambier J.C. EMBO J. 1994; 13: 1911-1919Google Scholar).Functional analysis of Ig-α and Ig-β cytoplasmic domains in B cells established that both are able to induce an increase of intracellular calcium concentration (15Sanchez M. Misulovin Z. Burkhardt A.L. Mahajan S. Costa T. Franke R. Bolen J.B. Nussenzweig M. J. Exp. Med. 1993; 178: 1049-1055Google Scholar, 16Kim K.M. Alber G. Weiser P. Reth M. Eur. J. Immunol. 1993; 23: 911-916Google Scholar, 17Taddie J.A. Hurley T.R. Hardwick B.S. Sefton B.M. J. Biol. Chem. 1994; 269: 13529-13535Google Scholar) with qualitative differences (18Choquet D. Ku G. Cassard S. Malissen B. Korn H. Fridman W.H. Bonnerot C. J. Biol. Chem. 1994; 269: 6491-6497Google Scholar). Although Ig-β was as efficient as Ig-α in triggering protein-tyrosine kinase activation, only Ig-α-containing chimeras were able to trigger an efficient signal transduction leading to an extracellular calcium influx and interleukin-2 (IL-2) production in the IIA1.6 B cell line. Ig-β triggered an oscillatory release from intracellular calcium stores and no IL-2 secretion (18Choquet D. Ku G. Cassard S. Malissen B. Korn H. Fridman W.H. Bonnerot C. J. Biol. Chem. 1994; 269: 6491-6497Google Scholar). Ig-α and Ig-β cytoplasmic domains therefore possess their own distinct signaling capabilities. In this study, the molecular basis of the different signaling capacities of Ig-α and Ig-β cytoplasmic tails were analyzed. We showed that BCR subunits transducing activities are based on the ITAM conserved sequence, but they may be regulated by unconserved sequences located inside or outside the motif." @default.
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