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- W1996336056 abstract "Mesoderm induction and patterning are primarily regulated by the concentration of locally expressed morphogens such as members of the TGFβsuperfamily. Smad2 functions as a transcription factor to regulate expression of mesodermal genes downstream of such morphogens. We have identified Xenopus PIASy (XPIASy), a member of the PIAS family, by yeast two-hybrid screening using Xenopus Smad2 (XSmad2) as a bait. During mesoderm induction, XPIASy is expressed in the animal half of embryos with a ventral high-dorsal low gradient at the marginal zone. XPIASyexpression is positively and negatively regulated by activities of the XSmad2 and Wnt pathways, respectively. Interestingly, inhibition of XPIASy by morpholinos induces elongation of animal caps with induction of mesoderm genes even in the absence of their morphogen-mediated activation. In addition, their introduction into the ventral marginal zone results in a secondary axis formation. Gain-of-function analysis revealed that XPIASy inhibits mesoderm induction by specific and direct downregulation of XSmad2 transcriptional activity. These observations indicate that XPIASy functions as an essential negative regulator of the XSmad2 pathway to ensure proper mesoderm induction at the appropriate time and in the appropriate region, and suggest that both the initial step of morphogen-mediated activation of the XSmad2 pathway and regulation of the final downstream transcription step have crucial roles in mesoderm induction and patterning." @default.
- W1996336056 created "2016-06-24" @default.
- W1996336056 creator A5003793915 @default.
- W1996336056 creator A5004979383 @default.
- W1996336056 creator A5008074600 @default.
- W1996336056 creator A5083885354 @default.
- W1996336056 date "2004-11-15" @default.
- W1996336056 modified "2023-10-10" @default.
- W1996336056 title "Negative regulation of Smad2 by PIASy is required for proper<i>Xenopus</i>mesoderm formation" @default.
- W1996336056 cites W1515719112 @default.
- W1996336056 cites W1529816420 @default.
- W1996336056 cites W1532872348 @default.
- W1996336056 cites W1553444759 @default.
- W1996336056 cites W1684737398 @default.
- W1996336056 cites W1720193968 @default.
- W1996336056 cites W1868158025 @default.
- W1996336056 cites W1900133754 @default.
- W1996336056 cites W1965344790 @default.
- W1996336056 cites W1969892750 @default.
- W1996336056 cites W1971611931 @default.
- W1996336056 cites W1971846249 @default.
- W1996336056 cites W1972449201 @default.
- W1996336056 cites W1985723042 @default.
- W1996336056 cites W1987647476 @default.
- W1996336056 cites W1989721036 @default.
- W1996336056 cites W1990758122 @default.
- W1996336056 cites W1992685753 @default.
- W1996336056 cites W1993154580 @default.
- W1996336056 cites W1995456120 @default.
- W1996336056 cites W1996811697 @default.
- W1996336056 cites W1997642091 @default.
- W1996336056 cites W1997858639 @default.
- W1996336056 cites W2002246876 @default.
- W1996336056 cites W2002898689 @default.
- W1996336056 cites W2003929062 @default.
- W1996336056 cites W2004214063 @default.
- W1996336056 cites W2008575029 @default.
- W1996336056 cites W2009989504 @default.
- W1996336056 cites W2010041789 @default.
- W1996336056 cites W2015087426 @default.
- W1996336056 cites W2015929447 @default.
- W1996336056 cites W2018454809 @default.
- W1996336056 cites W2018485286 @default.
- W1996336056 cites W2018631055 @default.
- W1996336056 cites W2023077752 @default.
- W1996336056 cites W2025123418 @default.
- W1996336056 cites W2025609408 @default.
- W1996336056 cites W2026436579 @default.
- W1996336056 cites W2028348480 @default.
- W1996336056 cites W2032316637 @default.
- W1996336056 cites W2035357982 @default.
- W1996336056 cites W2043377295 @default.
- W1996336056 cites W2046597024 @default.
- W1996336056 cites W2048264979 @default.
- W1996336056 cites W2048835534 @default.
- W1996336056 cites W2052096221 @default.
- W1996336056 cites W2052142480 @default.
- W1996336056 cites W2059030354 @default.
- W1996336056 cites W2060817283 @default.
- W1996336056 cites W2066696278 @default.
- W1996336056 cites W2073654240 @default.
- W1996336056 cites W2074773822 @default.
- W1996336056 cites W2077557886 @default.
- W1996336056 cites W2079092516 @default.
- W1996336056 cites W2079508317 @default.
- W1996336056 cites W2080546144 @default.
- W1996336056 cites W2082466352 @default.
- W1996336056 cites W2090272354 @default.
- W1996336056 cites W2090734530 @default.
- W1996336056 cites W2097160587 @default.
- W1996336056 cites W2101619133 @default.
- W1996336056 cites W2107469094 @default.
- W1996336056 cites W2109379227 @default.
- W1996336056 cites W2109834892 @default.
- W1996336056 cites W2112406299 @default.
- W1996336056 cites W2113786860 @default.
- W1996336056 cites W2115675121 @default.
- W1996336056 cites W2115686163 @default.
- W1996336056 cites W2116988305 @default.
- W1996336056 cites W2118810511 @default.
- W1996336056 cites W2121428233 @default.
- W1996336056 cites W2121779606 @default.
- W1996336056 cites W2122044343 @default.
- W1996336056 cites W2122398263 @default.
- W1996336056 cites W2126794028 @default.
- W1996336056 cites W2127597058 @default.
- W1996336056 cites W2131815302 @default.
- W1996336056 cites W2132584224 @default.
- W1996336056 cites W2142216488 @default.
- W1996336056 cites W2142656958 @default.
- W1996336056 cites W2146434687 @default.
- W1996336056 cites W2147403152 @default.
- W1996336056 cites W2154568166 @default.
- W1996336056 cites W2154897673 @default.
- W1996336056 cites W2156212940 @default.
- W1996336056 cites W2164563970 @default.
- W1996336056 cites W2183521217 @default.
- W1996336056 cites W2187553747 @default.