Matches in SemOpenAlex for { <https://semopenalex.org/work/W1996348518> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W1996348518 abstract "Sulfhydryl compounds can protect DNA against free-radical-induced DNA damages not only by scavenging of radicals, but also by chemical non-enzymatic repair or modification of such damages by hydrogen-donation. To investigate the influence of chemical repair and modification on mutations, induced by gamma-radiation-generated free radicals (.OH, .H), phosphate-buffered aqueous solutions of double-stranded (ds) M13 DNA were exposed to gamma-rays under N2 in the presence of 5 mM cysteamine. The exposed DNA was subsequently transfected to wild-type E. coli and mutations in the mutational target were characterized. This target in fact contains three different target sequences, i.e., the lac promoter/operator, the lacZ alpha gene and a 144 bp inframe insert. The mutation spectrum obtained was compared with those in the absence of cysteamine under N2 and N2O. In the latter case, the ratio of .OH and .H available for reacting with DNA is about the same as under N2 + cysteamine. The results show that chemical repair and/or modification by cysteamine of potentially lethal lesions takes place, leading to a much higher survival of ds M13 DNA in the presence of cysteamine than could be expected on basis of scavenging of .OH and .H alone. This higher survival appeared to be accompanied with a higher mutation induction. However, the N2 + cysteamine mutation spectrum shows a remarkable resemblance with the N2O-spectrum. This holds for the total mutation target, as well as each of the three targets, although the mutations obtained in each of the three targets under the same irradiation conditions are quite different. Thus, it can be concluded that cysteamine is mainly effective on radiation-induced potentially lethal DNA lesions, and not so much on (pre)mutagenic damages. Moreover, the type of mutation appeared to be strongly dependent on the mutational target sequence." @default.
- W1996348518 created "2016-06-24" @default.
- W1996348518 creator A5046801135 @default.
- W1996348518 creator A5055105229 @default.
- W1996348518 creator A5087405937 @default.
- W1996348518 creator A5087936139 @default.
- W1996348518 date "1996-12-01" @default.
- W1996348518 modified "2023-10-17" @default.
- W1996348518 title "Effect of the sulfhydryl compound cysteamine on gamma-radiation-induced mutations in double-stranded M13 DNA" @default.
- W1996348518 cites W1014033160 @default.
- W1996348518 cites W1240656434 @default.
- W1996348518 cites W148370306 @default.
- W1996348518 cites W1975129370 @default.
- W1996348518 cites W1982490883 @default.
- W1996348518 cites W1983250095 @default.
- W1996348518 cites W1991709262 @default.
- W1996348518 cites W2001070612 @default.
- W1996348518 cites W2002769318 @default.
- W1996348518 cites W2017626786 @default.
- W1996348518 cites W2036504440 @default.
- W1996348518 cites W2038459334 @default.
- W1996348518 cites W2042777161 @default.
- W1996348518 cites W2057252608 @default.
- W1996348518 cites W2065885923 @default.
- W1996348518 cites W2066752911 @default.
- W1996348518 cites W2067626253 @default.
- W1996348518 cites W2077017381 @default.
- W1996348518 cites W2078545495 @default.
- W1996348518 cites W2080763553 @default.
- W1996348518 cites W2087177372 @default.
- W1996348518 cites W2115989447 @default.
- W1996348518 cites W2118706141 @default.
- W1996348518 cites W2138270253 @default.
- W1996348518 cites W2202634750 @default.
- W1996348518 cites W2217117567 @default.
- W1996348518 doi "https://doi.org/10.1016/s0921-8777(96)00030-4" @default.
- W1996348518 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8960129" @default.
- W1996348518 hasPublicationYear "1996" @default.
- W1996348518 type Work @default.
- W1996348518 sameAs 1996348518 @default.
- W1996348518 citedByCount "10" @default.
- W1996348518 countsByYear W19963485182014 @default.
- W1996348518 countsByYear W19963485182021 @default.
- W1996348518 crossrefType "journal-article" @default.
- W1996348518 hasAuthorship W1996348518A5046801135 @default.
- W1996348518 hasAuthorship W1996348518A5055105229 @default.
- W1996348518 hasAuthorship W1996348518A5087405937 @default.
- W1996348518 hasAuthorship W1996348518A5087936139 @default.
- W1996348518 hasConcept C102306862 @default.
- W1996348518 hasConcept C104317684 @default.
- W1996348518 hasConcept C134935766 @default.
- W1996348518 hasConcept C139066938 @default.
- W1996348518 hasConcept C143425029 @default.
- W1996348518 hasConcept C153911025 @default.
- W1996348518 hasConcept C185592680 @default.
- W1996348518 hasConcept C2776609658 @default.
- W1996348518 hasConcept C2994225774 @default.
- W1996348518 hasConcept C501734568 @default.
- W1996348518 hasConcept C54009773 @default.
- W1996348518 hasConcept C552990157 @default.
- W1996348518 hasConcept C55493867 @default.
- W1996348518 hasConcept C86803240 @default.
- W1996348518 hasConceptScore W1996348518C102306862 @default.
- W1996348518 hasConceptScore W1996348518C104317684 @default.
- W1996348518 hasConceptScore W1996348518C134935766 @default.
- W1996348518 hasConceptScore W1996348518C139066938 @default.
- W1996348518 hasConceptScore W1996348518C143425029 @default.
- W1996348518 hasConceptScore W1996348518C153911025 @default.
- W1996348518 hasConceptScore W1996348518C185592680 @default.
- W1996348518 hasConceptScore W1996348518C2776609658 @default.
- W1996348518 hasConceptScore W1996348518C2994225774 @default.
- W1996348518 hasConceptScore W1996348518C501734568 @default.
- W1996348518 hasConceptScore W1996348518C54009773 @default.
- W1996348518 hasConceptScore W1996348518C552990157 @default.
- W1996348518 hasConceptScore W1996348518C55493867 @default.
- W1996348518 hasConceptScore W1996348518C86803240 @default.
- W1996348518 hasLocation W19963485181 @default.
- W1996348518 hasLocation W19963485182 @default.
- W1996348518 hasOpenAccess W1996348518 @default.
- W1996348518 hasPrimaryLocation W19963485181 @default.
- W1996348518 hasRelatedWork W1562559317 @default.
- W1996348518 hasRelatedWork W1949617809 @default.
- W1996348518 hasRelatedWork W1973247573 @default.
- W1996348518 hasRelatedWork W1986227390 @default.
- W1996348518 hasRelatedWork W2004498595 @default.
- W1996348518 hasRelatedWork W2053243299 @default.
- W1996348518 hasRelatedWork W2078912605 @default.
- W1996348518 hasRelatedWork W2413328596 @default.
- W1996348518 hasRelatedWork W2769468652 @default.
- W1996348518 hasRelatedWork W2954932260 @default.
- W1996348518 isParatext "false" @default.
- W1996348518 isRetracted "false" @default.
- W1996348518 magId "1996348518" @default.
- W1996348518 workType "article" @default.