FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1996387910> ?p ?o ?g. }

• W1996387910 endingPage "276" @default.
• W1996387910 startingPage "261" @default.
• W1996387910 abstract "Multiphoton micro-spectroscopy, employing diffraction optics and electron-multiplying CCD (EMCCD) cameras, is a suitable method for determining protein complex stoichiometry, quaternary structure, and spatial distribution in living cells using Förster resonance energy transfer (FRET) imaging. The method provides highly resolved spectra of molecules or molecular complexes at each image pixel, and it does so on a timescale shorter than that of molecular diffusion, which scrambles the spectral information. Acquisition of an entire spectrally resolved image, however, is slower than that of broad-bandwidth microscopes because it takes longer times to collect the same number of photons at each emission wavelength as in a broad bandwidth. Here, we demonstrate an optical micro-spectroscopic scheme that employs a laser beam shaped into a line to excite in parallel multiple sample voxels. The method presents dramatically increased sensitivity and/or acquisition speed and, at the same time, has excellent spatial and spectral resolution, similar to point-scan configurations. When applied to FRET imaging using an oligomeric FRET construct expressed in living cells and consisting of a FRET acceptor linked to three donors, the technique based on line-shaped excitation provides higher accuracy compared to the point-scan approach, and it reduces artifacts caused by photobleaching and other undesired photophysical effects." @default.
• W1996387910 created "2016-06-24" @default.
• W1996387910 creator A5003356590 @default.
• W1996387910 creator A5011973750 @default.
• W1996387910 creator A5023140771 @default.
• W1996387910 creator A5044629396 @default.
• W1996387910 creator A5057443152 @default.
• W1996387910 creator A5066237501 @default.
• W1996387910 creator A5075849412 @default.
• W1996387910 creator A5084013581 @default.
• W1996387910 date "2013-12-27" @default.
• W1996387910 modified "2023-10-17" @default.
• W1996387910 title "Development and Experimental Testing of an Optical Micro-Spectroscopic Technique Incorporating True Line-Scan Excitation" @default.
• W1996387910 cites W1487409748 @default.
• W1996387910 cites W1490295882 @default.
• W1996387910 cites W1509938302 @default.
• W1996387910 cites W1967890875 @default.
• W1996387910 cites W1970066028 @default.
• W1996387910 cites W1975717061 @default.
• W1996387910 cites W1982605456 @default.
• W1996387910 cites W1988606601 @default.
• W1996387910 cites W1991409871 @default.
• W1996387910 cites W1993325325 @default.
• W1996387910 cites W1996353817 @default.
• W1996387910 cites W1996552596 @default.
• W1996387910 cites W2003777386 @default.
• W1996387910 cites W2004483753 @default.
• W1996387910 cites W2005195616 @default.
• W1996387910 cites W2016370272 @default.
• W1996387910 cites W2018930967 @default.
• W1996387910 cites W2019074781 @default.
• W1996387910 cites W2023882972 @default.
• W1996387910 cites W2024406707 @default.
• W1996387910 cites W2027045807 @default.
• W1996387910 cites W2028019750 @default.
• W1996387910 cites W2034999042 @default.
• W1996387910 cites W2040723705 @default.
• W1996387910 cites W2055698508 @default.
• W1996387910 cites W2056410410 @default.
• W1996387910 cites W2058623136 @default.
• W1996387910 cites W2061464631 @default.
• W1996387910 cites W2065045053 @default.
• W1996387910 cites W2068613460 @default.
• W1996387910 cites W2072132049 @default.
• W1996387910 cites W2073059242 @default.
• W1996387910 cites W2085573815 @default.
• W1996387910 cites W2091655993 @default.
• W1996387910 cites W2094486254 @default.
• W1996387910 cites W2123789039 @default.
• W1996387910 cites W2127068865 @default.
• W1996387910 cites W2143651971 @default.
• W1996387910 cites W2149823296 @default.
• W1996387910 cites W2153684394 @default.
• W1996387910 cites W2155287512 @default.
• W1996387910 cites W2156814492 @default.
• W1996387910 cites W2162209822 @default.
• W1996387910 cites W2167155844 @default.
• W1996387910 cites W2167460696 @default.
• W1996387910 cites W2168283408 @default.
• W1996387910 cites W2090605768 @default.
• W1996387910 doi "https://doi.org/10.3390/ijms15010261" @default.
• W1996387910 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3907809" @default.
• W1996387910 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24378851" @default.
• W1996387910 hasPublicationYear "2013" @default.
• W1996387910 type Work @default.
• W1996387910 sameAs 1996387910 @default.
• W1996387910 citedByCount "52" @default.
• W1996387910 countsByYear W19963879102015 @default.
• W1996387910 countsByYear W19963879102016 @default.
• W1996387910 countsByYear W19963879102017 @default.
• W1996387910 countsByYear W19963879102018 @default.
• W1996387910 countsByYear W19963879102019 @default.
• W1996387910 countsByYear W19963879102020 @default.
• W1996387910 countsByYear W19963879102021 @default.
• W1996387910 countsByYear W19963879102022 @default.
• W1996387910 countsByYear W19963879102023 @default.
• W1996387910 crossrefType "journal-article" @default.
• W1996387910 hasAuthorship W1996387910A5003356590 @default.
• W1996387910 hasAuthorship W1996387910A5011973750 @default.
• W1996387910 hasAuthorship W1996387910A5023140771 @default.
• W1996387910 hasAuthorship W1996387910A5044629396 @default.
• W1996387910 hasAuthorship W1996387910A5057443152 @default.
• W1996387910 hasAuthorship W1996387910A5066237501 @default.
• W1996387910 hasAuthorship W1996387910A5075849412 @default.
• W1996387910 hasAuthorship W1996387910A5084013581 @default.
• W1996387910 hasBestOaLocation W19963879101 @default.
• W1996387910 hasConcept C120665830 @default.
• W1996387910 hasConcept C121332964 @default.
• W1996387910 hasConcept C147080431 @default.
• W1996387910 hasConcept C158479148 @default.
• W1996387910 hasConcept C185592680 @default.
• W1996387910 hasConcept C192562407 @default.
• W1996387910 hasConcept C205372480 @default.
• W1996387910 hasConcept C31332276 @default.
• W1996387910 hasConcept C3232514 @default.
• W1996387910 hasConcept C32891209 @default.
• W1996387910 hasConcept C520434653 @default.
• W1996387910 hasConcept C62520636 @default.

• next