Matches in SemOpenAlex for { <https://semopenalex.org/work/W1996391361> ?p ?o ?g. }
- W1996391361 endingPage "1014" @default.
- W1996391361 startingPage "1008" @default.
- W1996391361 abstract "Polyphenol oxidase (PPO) and peroxidase (POD) were extracted from a table grape (Crimson Seedless) using Triton X-114 and characterized using spectrophotometric methods. Both PPO and POD were activated by acid shock. However, in the presence of the anionic detergent sodium dodecil sulphate (SDS), PPO was activated whereas POD was inactivated. The enzymes were kinetically characterized and both followed Michaelis–Menten kinetics, although with different values of their kinetic parameters. The Vm/Km ratio showed that Crimson Seedless grape PPO presents a similar affinity for 4-tert-butyl-catechol (TBC) whether activated by acid shock (0.018 min−1) or SDS (0.023 min−1). With regards to POD, the Km and Vm values for 2,2′-azinobis(3-ethylbenzothiazolinesulphonic acid) (ABTS) were 0.79 mM and 1.20 μM/min, respectively. In the case of H2O2, the Km and Vm value were 0.4 mM and 0.93 μM/min, respectively. PPO and POD showed similar thermostability, losing >90% of relative activity after only 5 min of incubation at 78 °C and 75 °C, respectively. In addition, PPO´s activation energy was similar to that obtained for POD (295.5 kJ/mol and 271.9 kJ/mol, respectively)." @default.
- W1996391361 created "2016-06-24" @default.
- W1996391361 creator A5052226200 @default.
- W1996391361 creator A5058016306 @default.
- W1996391361 creator A5080925470 @default.
- W1996391361 creator A5085461326 @default.
- W1996391361 creator A5091131396 @default.
- W1996391361 date "2009-04-01" @default.
- W1996391361 modified "2023-09-26" @default.
- W1996391361 title "Kinetic characterisation and thermal inactivation study of polyphenol oxidase and peroxidase from table grape (Crimson Seedless)" @default.
- W1996391361 cites W1804721810 @default.
- W1996391361 cites W1949772429 @default.
- W1996391361 cites W1963833862 @default.
- W1996391361 cites W1964928280 @default.
- W1996391361 cites W1965859058 @default.
- W1996391361 cites W1969761762 @default.
- W1996391361 cites W1970968419 @default.
- W1996391361 cites W1977396441 @default.
- W1996391361 cites W1979023687 @default.
- W1996391361 cites W1982922084 @default.
- W1996391361 cites W1988266627 @default.
- W1996391361 cites W1997633025 @default.
- W1996391361 cites W1997916802 @default.
- W1996391361 cites W2004845723 @default.
- W1996391361 cites W2008760038 @default.
- W1996391361 cites W2010417748 @default.
- W1996391361 cites W2025743160 @default.
- W1996391361 cites W2031433984 @default.
- W1996391361 cites W2034032796 @default.
- W1996391361 cites W2039042657 @default.
- W1996391361 cites W2040268735 @default.
- W1996391361 cites W2043397618 @default.
- W1996391361 cites W2046730327 @default.
- W1996391361 cites W2051503189 @default.
- W1996391361 cites W2052925930 @default.
- W1996391361 cites W2054145587 @default.
- W1996391361 cites W2067046219 @default.
- W1996391361 cites W2068209657 @default.
- W1996391361 cites W2072649021 @default.
- W1996391361 cites W2073002882 @default.
- W1996391361 cites W2074548171 @default.
- W1996391361 cites W2076484020 @default.
- W1996391361 cites W2081106256 @default.
- W1996391361 cites W2086201757 @default.
- W1996391361 cites W2089317757 @default.
- W1996391361 cites W2094317380 @default.
- W1996391361 cites W2100725614 @default.
- W1996391361 cites W2107268560 @default.
- W1996391361 cites W2116351810 @default.
- W1996391361 cites W2137381184 @default.
- W1996391361 cites W2139772523 @default.
- W1996391361 cites W2145012195 @default.
- W1996391361 cites W2474787756 @default.
- W1996391361 cites W4293247451 @default.
- W1996391361 cites W55034282 @default.
- W1996391361 doi "https://doi.org/10.1016/j.foodchem.2008.08.053" @default.
- W1996391361 hasPublicationYear "2009" @default.
- W1996391361 type Work @default.
- W1996391361 sameAs 1996391361 @default.
- W1996391361 citedByCount "76" @default.
- W1996391361 countsByYear W19963913612012 @default.
- W1996391361 countsByYear W19963913612013 @default.
- W1996391361 countsByYear W19963913612014 @default.
- W1996391361 countsByYear W19963913612015 @default.
- W1996391361 countsByYear W19963913612016 @default.
- W1996391361 countsByYear W19963913612017 @default.
- W1996391361 countsByYear W19963913612018 @default.
- W1996391361 countsByYear W19963913612019 @default.
- W1996391361 countsByYear W19963913612020 @default.
- W1996391361 countsByYear W19963913612021 @default.
- W1996391361 countsByYear W19963913612022 @default.
- W1996391361 countsByYear W19963913612023 @default.
- W1996391361 crossrefType "journal-article" @default.
- W1996391361 hasAuthorship W1996391361A5052226200 @default.
- W1996391361 hasAuthorship W1996391361A5058016306 @default.
- W1996391361 hasAuthorship W1996391361A5080925470 @default.
- W1996391361 hasAuthorship W1996391361A5085461326 @default.
- W1996391361 hasAuthorship W1996391361A5091131396 @default.
- W1996391361 hasBestOaLocation W19963913612 @default.
- W1996391361 hasConcept C137776501 @default.
- W1996391361 hasConcept C13965031 @default.
- W1996391361 hasConcept C144027150 @default.
- W1996391361 hasConcept C162008176 @default.
- W1996391361 hasConcept C181199279 @default.
- W1996391361 hasConcept C185592680 @default.
- W1996391361 hasConcept C2776338311 @default.
- W1996391361 hasConcept C2777713698 @default.
- W1996391361 hasConcept C2778004101 @default.
- W1996391361 hasConcept C2778920651 @default.
- W1996391361 hasConcept C2781031786 @default.
- W1996391361 hasConcept C2781216036 @default.
- W1996391361 hasConcept C2781401929 @default.
- W1996391361 hasConcept C31903555 @default.
- W1996391361 hasConcept C41183919 @default.
- W1996391361 hasConcept C43617362 @default.
- W1996391361 hasConcept C55493867 @default.
- W1996391361 hasConcept C56856141 @default.
- W1996391361 hasConcept C58808276 @default.