Matches in SemOpenAlex for { <https://semopenalex.org/work/W1996403602> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W1996403602 endingPage "139" @default.
- W1996403602 startingPage "134" @default.
- W1996403602 abstract "Aspergillus niger mutant 817 was generated from A. niger 12, a strain that produces high levels of inulinases irrespective of the carbon source, by γ-irradiation with 60Co. A. niger 817 showed 4-fold higher inulinase activity than the wild-type strain 12 in the filtrate of a submerged culture with fructose. An extremely high ratio of inulinase to invertase activity (I/S ratio) of 880 was found in the culture filtrate. Two inulinases, P-IA and P-IB, were purified from the culture filtrate of A. niger 817 grown on sucrose by DEAE-Cellulofine A-500 and Q-Sepharose HP chromatographies. The enzymes were homogeneous as judged by SDS-polyacrylamide gel electrophoresis with apparent molecular weights of 70,000 for P-IA and 68,000 for P-IB. The specific activities were 350 U/mg for P-IA and 340 U/mg for P-IB, and about 3.5-fold higher than that reported for the wild-type endoinulinase P-III. The enzymes were active only toward inulin, and lacked activity toward sucrose, raffinose or levan. The main products of inulin hydrolysis were inulotriose and -tetraose. Thus, both inulinases exhibited the endo-type mode of action on inulin. The apparent Km values for inulin were 0.48 mM for P-IA and 0.50 mM for P-IB at 40°C and pH 5.0, and lower than the 1.25 mM reported for the wild-type endoinulinase P-III." @default.
- W1996403602 created "2016-06-24" @default.
- W1996403602 creator A5040400395 @default.
- W1996403602 creator A5056739586 @default.
- W1996403602 creator A5060790153 @default.
- W1996403602 creator A5073188851 @default.
- W1996403602 creator A5087070509 @default.
- W1996403602 date "1994-01-01" @default.
- W1996403602 modified "2023-09-26" @default.
- W1996403602 title "Occurrence of two forms of extracellular endoinulinase from Aspergillus niger mutant 817" @default.
- W1996403602 cites W1125851231 @default.
- W1996403602 cites W1656254547 @default.
- W1996403602 cites W1775749144 @default.
- W1996403602 cites W1974050937 @default.
- W1996403602 cites W1986064211 @default.
- W1996403602 cites W1994224244 @default.
- W1996403602 cites W2004330382 @default.
- W1996403602 cites W2009674410 @default.
- W1996403602 cites W2035800379 @default.
- W1996403602 cites W2054979333 @default.
- W1996403602 cites W2060857847 @default.
- W1996403602 cites W207776502 @default.
- W1996403602 cites W2100837269 @default.
- W1996403602 cites W2315833145 @default.
- W1996403602 cites W2331359381 @default.
- W1996403602 cites W2584857702 @default.
- W1996403602 cites W4243696764 @default.
- W1996403602 cites W4249124436 @default.
- W1996403602 cites W4250252729 @default.
- W1996403602 doi "https://doi.org/10.1016/0922-338x(94)90251-8" @default.
- W1996403602 hasPublicationYear "1994" @default.
- W1996403602 type Work @default.
- W1996403602 sameAs 1996403602 @default.
- W1996403602 citedByCount "61" @default.
- W1996403602 countsByYear W19964036022012 @default.
- W1996403602 countsByYear W19964036022014 @default.
- W1996403602 countsByYear W19964036022015 @default.
- W1996403602 countsByYear W19964036022016 @default.
- W1996403602 countsByYear W19964036022017 @default.
- W1996403602 countsByYear W19964036022019 @default.
- W1996403602 countsByYear W19964036022020 @default.
- W1996403602 countsByYear W19964036022021 @default.
- W1996403602 crossrefType "journal-article" @default.
- W1996403602 hasAuthorship W1996403602A5040400395 @default.
- W1996403602 hasAuthorship W1996403602A5056739586 @default.
- W1996403602 hasAuthorship W1996403602A5060790153 @default.
- W1996403602 hasAuthorship W1996403602A5073188851 @default.
- W1996403602 hasAuthorship W1996403602A5087070509 @default.
- W1996403602 hasConcept C104317684 @default.
- W1996403602 hasConcept C143065580 @default.
- W1996403602 hasConcept C181199279 @default.
- W1996403602 hasConcept C185592680 @default.
- W1996403602 hasConcept C2775960407 @default.
- W1996403602 hasConcept C2776970464 @default.
- W1996403602 hasConcept C2778597767 @default.
- W1996403602 hasConcept C2779742124 @default.
- W1996403602 hasConcept C2779998883 @default.
- W1996403602 hasConcept C2780236525 @default.
- W1996403602 hasConcept C31903555 @default.
- W1996403602 hasConcept C43617362 @default.
- W1996403602 hasConcept C55493867 @default.
- W1996403602 hasConcept C94412978 @default.
- W1996403602 hasConceptScore W1996403602C104317684 @default.
- W1996403602 hasConceptScore W1996403602C143065580 @default.
- W1996403602 hasConceptScore W1996403602C181199279 @default.
- W1996403602 hasConceptScore W1996403602C185592680 @default.
- W1996403602 hasConceptScore W1996403602C2775960407 @default.
- W1996403602 hasConceptScore W1996403602C2776970464 @default.
- W1996403602 hasConceptScore W1996403602C2778597767 @default.
- W1996403602 hasConceptScore W1996403602C2779742124 @default.
- W1996403602 hasConceptScore W1996403602C2779998883 @default.
- W1996403602 hasConceptScore W1996403602C2780236525 @default.
- W1996403602 hasConceptScore W1996403602C31903555 @default.
- W1996403602 hasConceptScore W1996403602C43617362 @default.
- W1996403602 hasConceptScore W1996403602C55493867 @default.
- W1996403602 hasConceptScore W1996403602C94412978 @default.
- W1996403602 hasIssue "2" @default.
- W1996403602 hasLocation W19964036021 @default.
- W1996403602 hasOpenAccess W1996403602 @default.
- W1996403602 hasPrimaryLocation W19964036021 @default.
- W1996403602 hasRelatedWork W1517901437 @default.
- W1996403602 hasRelatedWork W1695353026 @default.
- W1996403602 hasRelatedWork W1996403602 @default.
- W1996403602 hasRelatedWork W2017894710 @default.
- W1996403602 hasRelatedWork W2048589958 @default.
- W1996403602 hasRelatedWork W2064574993 @default.
- W1996403602 hasRelatedWork W2134457396 @default.
- W1996403602 hasRelatedWork W2181569659 @default.
- W1996403602 hasRelatedWork W2289552034 @default.
- W1996403602 hasRelatedWork W2416727314 @default.
- W1996403602 hasVolume "78" @default.
- W1996403602 isParatext "false" @default.
- W1996403602 isRetracted "false" @default.
- W1996403602 magId "1996403602" @default.
- W1996403602 workType "article" @default.