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- W1996451010 abstract "Functional DNA scaffolds can be defined as DNA-based structures comprising chemical moieties facilitating and guiding the immobilization of additional nanocomponents. Due to the limited reactivity of DNA there is currently a need to develop rapid routes to expand its chemical repertoire and increase its versatility as a nanostructuring scaffold. We report a simple synthetic strategy for generating linear and stable double-stranded DNA scaffolds functionalized with multiple sites reactive towards free thiols, and the utility of this approach is demonstrated by immobilizing a model protein containing an accessible free thiol. This procedure is very versatile and could be easily expanded to other types of chemistries. This approach could also potentially be employed for the specific, oriented immobilization of various biomolecules and nanoparticles on predefined DNA architectures." @default.
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- W1996451010 date "2009-05-18" @default.
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- W1996451010 title "A one-pot functionalization strategy for immobilizing proteins onto linear dsDNA scaffolds" @default.
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- W1996451010 doi "https://doi.org/10.1088/0957-4484/20/23/235101" @default.
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