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• W1996484080 abstract "A novel immobilized metal affinity membrane was prepared for papain adsorption in this article. Higher papain adsorption capacity between 43-67 mg/g was observed and the adsorption isotherm fitted the Freundlich equation. Experimental data were analyzed using two adsorption kinetic models. The pseudo-second-order kinetic model provided better correlation to the experimental results. A significant amount of the adsorbed papain was eluted by 1.0 M NaSCN at pH 5.0 for all affinity membranes. It was concluded that the novel chitosan-coated nylon-based immobilized metal ion affinity membrane could be applied for the large-scale isolation of papain without resulting in enzyme denaturation." @default.
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• W1996484080 date "2010-02-25" @default.
• W1996484080 modified "2023-09-25" @default.
• W1996484080 title "Papain Adsorption on Chitosan-Coated Nylon-Based Immobilized Metal Ion (Cu²⁺, Ni²⁺, Zn²⁺, Co²⁺) Affinity Membranes" @default.
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• W1996484080 doi "https://doi.org/10.1080/01496390903484784" @default.
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