FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1996561943> ?p ?o ?g. }

• W1996561943 endingPage "024002" @default.
• W1996561943 startingPage "024002" @default.
• W1996561943 abstract "Fluorescence proteins are widely used as markers for biomedical and technological purposes. Therefore, the aim of this project was to create a fluorescent sensor, based in the green and cyan fluorescent protein, using bacterial S-layers proteins as scaffold for the fluorescent tag. We report the cloning, expression and purification of three S-layer fluorescent proteins: SgsE-EGFP, SgsE-ECFP and SgsE-13aa-ECFP, this last containing a 13-amino acid rigid linker. The pH dependence of the fluorescence intensity of the S-layer fusion proteins, monitored by fluorescence spectroscopy, showed that the ECFP tag was more stable than EGFP. Furthermore, the fluorescent fusion proteins were reassembled on silica particles modified with cationic and anionic polyelectrolytes. Zeta potential measurements confirmed the particle coatings and indicated their colloidal stability. Flow cytometry and fluorescence microscopy showed that the fluorescence of the fusion proteins was pH dependent and sensitive to the underlying polyelectrolyte coating. This might suggest that the fluorescent tag is not completely exposed to the bulk media as an independent moiety. Finally, it was found out that viscosity enhanced the fluorescence intensity of the three fluorescent S-layer proteins." @default.
• W1996561943 created "2016-06-24" @default.
• W1996561943 creator A5008258219 @default.
• W1996561943 creator A5020717643 @default.
• W1996561943 creator A5025735101 @default.
• W1996561943 creator A5030032694 @default.
• W1996561943 creator A5052082810 @default.
• W1996561943 date "2014-04-10" @default.
• W1996561943 modified "2023-09-27" @default.
• W1996561943 title "Fluorescent sensors based on bacterial fusion proteins" @default.
• W1996561943 cites W144567121 @default.
• W1996561943 cites W1501566966 @default.
• W1996561943 cites W1598680929 @default.
• W1996561943 cites W1965479248 @default.
• W1996561943 cites W1971604188 @default.
• W1996561943 cites W1973574290 @default.
• W1996561943 cites W1979129905 @default.
• W1996561943 cites W1992906079 @default.
• W1996561943 cites W1999923843 @default.
• W1996561943 cites W2011081586 @default.
• W1996561943 cites W2012425908 @default.
• W1996561943 cites W2030239671 @default.
• W1996561943 cites W2039930060 @default.
• W1996561943 cites W2053004540 @default.
• W1996561943 cites W2057668947 @default.
• W1996561943 cites W2061680337 @default.
• W1996561943 cites W2068487080 @default.
• W1996561943 cites W2070423089 @default.
• W1996561943 cites W2087735060 @default.
• W1996561943 cites W2094704598 @default.
• W1996561943 cites W2112579958 @default.
• W1996561943 cites W2117208350 @default.
• W1996561943 cites W2127068865 @default.
• W1996561943 cites W2138922410 @default.
• W1996561943 cites W2140776957 @default.
• W1996561943 cites W2153469207 @default.
• W1996561943 cites W2165672384 @default.
• W1996561943 cites W2334231453 @default.
• W1996561943 cites W2491458126 @default.
• W1996561943 cites W25761802 @default.
• W1996561943 cites W4230350617 @default.
• W1996561943 cites W4241445517 @default.
• W1996561943 doi "https://doi.org/10.1088/2050-6120/2/2/024002" @default.
• W1996561943 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29148466" @default.
• W1996561943 hasPublicationYear "2014" @default.
• W1996561943 type Work @default.
• W1996561943 sameAs 1996561943 @default.
• W1996561943 citedByCount "3" @default.
• W1996561943 countsByYear W19965619432014 @default.
• W1996561943 countsByYear W19965619432016 @default.
• W1996561943 countsByYear W19965619432018 @default.
• W1996561943 crossrefType "journal-article" @default.
• W1996561943 hasAuthorship W1996561943A5008258219 @default.
• W1996561943 hasAuthorship W1996561943A5020717643 @default.
• W1996561943 hasAuthorship W1996561943A5025735101 @default.
• W1996561943 hasAuthorship W1996561943A5030032694 @default.
• W1996561943 hasAuthorship W1996561943A5052082810 @default.
• W1996561943 hasConcept C104317684 @default.
• W1996561943 hasConcept C121332964 @default.
• W1996561943 hasConcept C123894998 @default.
• W1996561943 hasConcept C12554922 @default.
• W1996561943 hasConcept C142613039 @default.
• W1996561943 hasConcept C153356533 @default.
• W1996561943 hasConcept C169274487 @default.
• W1996561943 hasConcept C178790620 @default.
• W1996561943 hasConcept C185592680 @default.
• W1996561943 hasConcept C40767141 @default.
• W1996561943 hasConcept C521977710 @default.
• W1996561943 hasConcept C55493867 @default.
• W1996561943 hasConcept C62520636 @default.
• W1996561943 hasConcept C86803240 @default.
• W1996561943 hasConcept C91881484 @default.
• W1996561943 hasConceptScore W1996561943C104317684 @default.
• W1996561943 hasConceptScore W1996561943C121332964 @default.
• W1996561943 hasConceptScore W1996561943C123894998 @default.
• W1996561943 hasConceptScore W1996561943C12554922 @default.
• W1996561943 hasConceptScore W1996561943C142613039 @default.
• W1996561943 hasConceptScore W1996561943C153356533 @default.
• W1996561943 hasConceptScore W1996561943C169274487 @default.
• W1996561943 hasConceptScore W1996561943C178790620 @default.
• W1996561943 hasConceptScore W1996561943C185592680 @default.
• W1996561943 hasConceptScore W1996561943C40767141 @default.
• W1996561943 hasConceptScore W1996561943C521977710 @default.
• W1996561943 hasConceptScore W1996561943C55493867 @default.
• W1996561943 hasConceptScore W1996561943C62520636 @default.
• W1996561943 hasConceptScore W1996561943C86803240 @default.
• W1996561943 hasConceptScore W1996561943C91881484 @default.
• W1996561943 hasIssue "2" @default.
• W1996561943 hasLocation W19965619431 @default.
• W1996561943 hasLocation W19965619432 @default.
• W1996561943 hasOpenAccess W1996561943 @default.
• W1996561943 hasPrimaryLocation W19965619431 @default.
• W1996561943 hasRelatedWork W1996561943 @default.
• W1996561943 hasRelatedWork W2012415189 @default.
• W1996561943 hasRelatedWork W2040694939 @default.
• W1996561943 hasRelatedWork W2069695895 @default.
• W1996561943 hasRelatedWork W2104892067 @default.
• W1996561943 hasRelatedWork W2151877111 @default.

• next