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- W1996564102 abstract "This report further characterises a new procedure for the preparation of affinity chromatographic supports, namely the activation of hydroxylic solid-phase supports with 1,1′-carbonyldiimidazole (CDI). Matrices with a controlled degree of substitution can be synthesised by the use of CDI, and if required, a high level of activation can readily be achieved e.g. up to 5 mmol/dry gram with cross-linked agarose. The CDI-activated agarose was found to have a half-life of greater than fourteen weeks when stored in dioxane. The conditions for coupling simple amines of differing pKa values to these active matrices were evaluated and the coupling yields analysed. Based on these results, conditions suitable for the coupling of proteins were established. The linkage of the amino group of the ligand to the support (an N-alkylcarbamate) was shown to possess good stability over a wide pH range. This stability was much greater than that of the isourea linkage obtained with the cyanogen bromide activation method. It is expected that this new activation procedure should prove to be particularly useful for a variety of affinity chromatographic experiments, including those which cannot tolerate the hydrolytic release of small amounts of the insolubilised ligand. The CDI method has been extended to other polysaccharide matrices e.g. cellulose and dextran derivatives, and to glycophase-coated glass beads. The activated glass bead derivative provides a suitable support for attachment of ligands for high-performance affinity chromatography." @default.
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- W1996564102 date "1987-12-01" @default.
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- W1996564102 title "Investigation of the activation of various insoluble polysaccharides with 1,1′-carbonyldiimidazole and of the properties of the activated matrices" @default.
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- W1996564102 doi "https://doi.org/10.1016/s0021-9673(00)80379-9" @default.
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