FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1996571710> ?p ?o ?g. }

• W1996571710 abstract "Background: Homocysteine is a sulfur-containing amino acid which formed (mainly in the liver) during the metabolism of methionine. Prior studies indicated the important role of hyperhomocysteinemia in pathogenesis and progression of alcoholic liver disease, liver steatosis and cirrhosis. One of the most important mechanisms by which homocysteine promote the development of hepatic injury is oxidative stress. Transcription factor Nrf2-mediated antioxidant response, represents critical cellular defense mechanism that serves to maintain intracellular redox homeostasis and limit oxidative stress. Glutamate cysteine ligase catalytic (GCLc) is rate limiting enzyme in the synthesis of glutathione, an important endogenous antioxidant. Objectives: This study was conducted to investigate whether homocysteine induces the Nrf2 dependent expression of GCLc in hepatoma cell line (HepG2) and whether this induction is mediated by antioxidant response element (ARE) which present within its promoter. Materials and Methods: Glutathione (GSH) content was measured by flow cytometry. Using electro mobility shift assay (EMSA) and western blotting, ARE-binding activity of Nrf2 for GCLc was demonstrated. Real time RT-PCR and western blotting were performed to evaluate whether homocysteine was able to induce mRNA and protein expression of GCL. Results: Exposure of HepG2 cells to 50 µMD/L homocysteine and western blotting of nuclear extracts revealed that Nrf2 is strongly stabilized and became detectable in nuclear extracts. EMSA demonstrated increased binding of Nrf2 to oligomers containing GCL promoter - specific ARE -binding site.A time- dependent increase in the gene and protein expression of GCL was observed. Additionally, GSH, which is prime scavenger of free radicals in cells, decreased initially. Elevation of GSH, following the initial decline, closely correlated with gene expression profile of GCLc, which is a rate-limiting enzyme in GSH synthesis. Conclusions: Altogether, we provide direct evidence that homocysteine activates Nrf2-mediated antioxidant response, which protects HepG2 cells from oxidative damage." @default.
• W1996571710 created "2016-06-24" @default.
• W1996571710 creator A5002300449 @default.
• W1996571710 creator A5012391705 @default.
• W1996571710 creator A5047959063 @default.
• W1996571710 creator A5056399499 @default.
• W1996571710 creator A5059611066 @default.
• W1996571710 creator A5061642917 @default.
• W1996571710 creator A5073934778 @default.
• W1996571710 creator A5088377096 @default.
• W1996571710 date "2013-04-20" @default.
• W1996571710 modified "2023-09-25" @default.
• W1996571710 title "Activation of Nrf2-Antioxidant Response Element Mediated Glutamate Cysteine Ligase Expression in Hepatoma Cell line by Homocysteine" @default.
• W1996571710 cites W124501502 @default.
• W1996571710 cites W1969464957 @default.
• W1996571710 cites W1985255011 @default.
• W1996571710 cites W1994426949 @default.
• W1996571710 cites W2000451610 @default.
• W1996571710 cites W2011866072 @default.
• W1996571710 cites W2015243552 @default.
• W1996571710 cites W2015312591 @default.
• W1996571710 cites W2019544413 @default.
• W1996571710 cites W2020286605 @default.
• W1996571710 cites W2028766244 @default.
• W1996571710 cites W2035393104 @default.
• W1996571710 cites W2043038263 @default.
• W1996571710 cites W2046328880 @default.
• W1996571710 cites W2051019972 @default.
• W1996571710 cites W2060388817 @default.
• W1996571710 cites W2064512387 @default.
• W1996571710 cites W2073925042 @default.
• W1996571710 cites W2076782602 @default.
• W1996571710 cites W2079566392 @default.
• W1996571710 cites W2089097264 @default.
• W1996571710 cites W2094500737 @default.
• W1996571710 cites W2104140554 @default.
• W1996571710 cites W2135719628 @default.
• W1996571710 cites W2136755864 @default.
• W1996571710 cites W2137234652 @default.
• W1996571710 cites W2140455030 @default.
• W1996571710 cites W2161607162 @default.
• W1996571710 cites W2164721380 @default.
• W1996571710 doi "https://doi.org/10.5812/hepatmon.8394" @default.
• W1996571710 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3743301" @default.
• W1996571710 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23967023" @default.
• W1996571710 hasPublicationYear "2013" @default.
• W1996571710 type Work @default.
• W1996571710 sameAs 1996571710 @default.
• W1996571710 citedByCount "38" @default.
• W1996571710 countsByYear W19965717102014 @default.
• W1996571710 countsByYear W19965717102015 @default.
• W1996571710 countsByYear W19965717102016 @default.
• W1996571710 countsByYear W19965717102017 @default.
• W1996571710 countsByYear W19965717102018 @default.
• W1996571710 countsByYear W19965717102019 @default.
• W1996571710 countsByYear W19965717102020 @default.
• W1996571710 countsByYear W19965717102021 @default.
• W1996571710 countsByYear W19965717102022 @default.
• W1996571710 crossrefType "journal-article" @default.
• W1996571710 hasAuthorship W1996571710A5002300449 @default.
• W1996571710 hasAuthorship W1996571710A5012391705 @default.
• W1996571710 hasAuthorship W1996571710A5047959063 @default.
• W1996571710 hasAuthorship W1996571710A5056399499 @default.
• W1996571710 hasAuthorship W1996571710A5059611066 @default.
• W1996571710 hasAuthorship W1996571710A5061642917 @default.
• W1996571710 hasAuthorship W1996571710A5073934778 @default.
• W1996571710 hasAuthorship W1996571710A5088377096 @default.
• W1996571710 hasBestOaLocation W19965717101 @default.
• W1996571710 hasConcept C126322002 @default.
• W1996571710 hasConcept C153911025 @default.
• W1996571710 hasConcept C181199279 @default.
• W1996571710 hasConcept C185592680 @default.
• W1996571710 hasConcept C2776151105 @default.
• W1996571710 hasConcept C2777090595 @default.
• W1996571710 hasConcept C2777214474 @default.
• W1996571710 hasConcept C2777575235 @default.
• W1996571710 hasConcept C2779881523 @default.
• W1996571710 hasConcept C2781173740 @default.
• W1996571710 hasConcept C538909803 @default.
• W1996571710 hasConcept C55493867 @default.
• W1996571710 hasConcept C71924100 @default.
• W1996571710 hasConcept C86803240 @default.
• W1996571710 hasConceptScore W1996571710C126322002 @default.
• W1996571710 hasConceptScore W1996571710C153911025 @default.
• W1996571710 hasConceptScore W1996571710C181199279 @default.
• W1996571710 hasConceptScore W1996571710C185592680 @default.
• W1996571710 hasConceptScore W1996571710C2776151105 @default.
• W1996571710 hasConceptScore W1996571710C2777090595 @default.
• W1996571710 hasConceptScore W1996571710C2777214474 @default.
• W1996571710 hasConceptScore W1996571710C2777575235 @default.
• W1996571710 hasConceptScore W1996571710C2779881523 @default.
• W1996571710 hasConceptScore W1996571710C2781173740 @default.
• W1996571710 hasConceptScore W1996571710C538909803 @default.
• W1996571710 hasConceptScore W1996571710C55493867 @default.
• W1996571710 hasConceptScore W1996571710C71924100 @default.
• W1996571710 hasConceptScore W1996571710C86803240 @default.
• W1996571710 hasIssue "5" @default.
• W1996571710 hasLocation W19965717101 @default.
• W1996571710 hasLocation W19965717102 @default.
• W1996571710 hasLocation W19965717103 @default.

• next