FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1996580937> ?p ?o ?g. }

• W1996580937 endingPage "1256" @default.
• W1996580937 startingPage "1249" @default.
• W1996580937 abstract "Abstract Gene expression analysis using microarrays has proved to be an important method in life science. The opportunity to grow higher plants on the International Space Station (ISS) opens up the possibility for gene expression profiling of plants grown in microgravity. The work presented focuses on how to meet the scientific requirements of plant growth and the sample preservation, given the technical and operational constraints associated with space research. The growth chamber (Multigen-2 Science Testing Unit) and a protocol suggested to be used in the European Modular Cultivation System (EMCS) Multigen-2 experiment on the ISS to grow and later preserve Arabidopsis seedlings, were tested on ground. The results showed that most of the plants developed normally. In order to avoid high population stress the number of seedlings per growth area should be reduced. The RNAlater preservation method to be used in the space experiment was compared with a quick freeze in Liquid Nitrogen (LN2). The RNA from samples preserved in RNAlater at room temperature for 24 h was slightly more degraded than the RNA from the LN2 preserved samples (RNA integrity number, RIN: 7.7 and 8.6, respectively). However, the RNA quality and quantity was satisfactory for microarray analysis. Of the genes analysed, 74 genes (0.28%) were significantly differentially expressed, most of them showing moderate to low regulation. Among the genes induced in the RNAlater preserved samples, three salt inducible transcription factors (ZAT10, SZF1 and SZF2) were identified, suggesting that the high salt concentration in RNAlater causes salt stress before the transcription stopped. In conclusion, the Multigen-2 preservation protocol tested here will allow for the genes regulated by microgravity in the space experiment to be revealed. The results do indicate that not all the biological processes are stopped instantly by the RNAlater. The limited diffusion indirectly caused by the microgravity may potentially result in a different degree of salt stress in the test compared to the 1 × g control during the space experiment. This has to be accounted for during the evaluation of the results. Since slightly degraded RNA was observed, further optimalisation of the preservation protocol will be performed." @default.
• W1996580937 created "2016-06-24" @default.
• W1996580937 creator A5000817009 @default.
• W1996580937 creator A5024041014 @default.
• W1996580937 creator A5046372204 @default.
• W1996580937 creator A5091810612 @default.
• W1996580937 date "2010-11-01" @default.
• W1996580937 modified "2023-09-27" @default.
• W1996580937 title "Ground testing of Arabidopsis preservation protocol for the microarray analysis to be used in the ISS EMCS Multigen-2 experiment" @default.
• W1996580937 cites W1972857165 @default.
• W1996580937 cites W1979178971 @default.
• W1996580937 cites W1983307768 @default.
• W1996580937 cites W1990643518 @default.
• W1996580937 cites W1991914426 @default.
• W1996580937 cites W2020686771 @default.
• W1996580937 cites W2028206196 @default.
• W1996580937 cites W2049589708 @default.
• W1996580937 cites W2050543866 @default.
• W1996580937 cites W2064172002 @default.
• W1996580937 cites W2086491289 @default.
• W1996580937 cites W2096481570 @default.
• W1996580937 cites W2099147258 @default.
• W1996580937 cites W2108244474 @default.
• W1996580937 cites W2109891367 @default.
• W1996580937 cites W2143420254 @default.
• W1996580937 cites W2150890381 @default.
• W1996580937 cites W2150926065 @default.
• W1996580937 cites W2155182166 @default.
• W1996580937 cites W2159675211 @default.
• W1996580937 cites W2163662566 @default.
• W1996580937 cites W2169684044 @default.
• W1996580937 cites W2236794485 @default.
• W1996580937 cites W4241639017 @default.
• W1996580937 doi "https://doi.org/10.1016/j.asr.2010.06.021" @default.
• W1996580937 hasPublicationYear "2010" @default.
• W1996580937 type Work @default.
• W1996580937 sameAs 1996580937 @default.
• W1996580937 citedByCount "9" @default.
• W1996580937 countsByYear W19965809372013 @default.
• W1996580937 countsByYear W19965809372014 @default.
• W1996580937 countsByYear W19965809372015 @default.
• W1996580937 countsByYear W19965809372016 @default.
• W1996580937 countsByYear W19965809372021 @default.
• W1996580937 countsByYear W19965809372022 @default.
• W1996580937 countsByYear W19965809372023 @default.
• W1996580937 crossrefType "journal-article" @default.
• W1996580937 hasAuthorship W1996580937A5000817009 @default.
• W1996580937 hasAuthorship W1996580937A5024041014 @default.
• W1996580937 hasAuthorship W1996580937A5046372204 @default.
• W1996580937 hasAuthorship W1996580937A5091810612 @default.
• W1996580937 hasConcept C142724271 @default.
• W1996580937 hasConcept C204787440 @default.
• W1996580937 hasConcept C2780385302 @default.
• W1996580937 hasConcept C41008148 @default.
• W1996580937 hasConcept C71924100 @default.
• W1996580937 hasConceptScore W1996580937C142724271 @default.
• W1996580937 hasConceptScore W1996580937C204787440 @default.
• W1996580937 hasConceptScore W1996580937C2780385302 @default.
• W1996580937 hasConceptScore W1996580937C41008148 @default.
• W1996580937 hasConceptScore W1996580937C71924100 @default.
• W1996580937 hasIssue "10" @default.
• W1996580937 hasLocation W19965809371 @default.
• W1996580937 hasOpenAccess W1996580937 @default.
• W1996580937 hasPrimaryLocation W19965809371 @default.
• W1996580937 hasRelatedWork W1500064003 @default.
• W1996580937 hasRelatedWork W1516169988 @default.
• W1996580937 hasRelatedWork W1596157658 @default.
• W1996580937 hasRelatedWork W196405095 @default.
• W1996580937 hasRelatedWork W2028024605 @default.
• W1996580937 hasRelatedWork W2049172720 @default.
• W1996580937 hasRelatedWork W2370697888 @default.
• W1996580937 hasRelatedWork W2373532818 @default.
• W1996580937 hasRelatedWork W2375403706 @default.
• W1996580937 hasRelatedWork W2562070253 @default.
• W1996580937 hasVolume "46" @default.
• W1996580937 isParatext "false" @default.
• W1996580937 isRetracted "false" @default.
• W1996580937 magId "1996580937" @default.
• W1996580937 workType "article" @default.