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- W1996602885 abstract "Female mice were superovulated and mated, and the two-cell embryos were collected and cultured in vitro. The embryos were exposed to x-irradiation (0–491 rads) during the two-cell stage, before the appearance of the next cleavage plate, placed in new unirradiated culture medium and observed during subsequent development. Morphological abnormalities which occurred as a result of irradiation included fragmentation, disintegration, granulation, incomplete cleavage, cleavage cessation, nuclear degeneration and pycnosis and cytoplasmic vacuolization. There was no damage to the zona pellucida. The types of abnormalities indicated an agreement with the results of previous in vivo studies. A distinct correlation existed between morphological abnormalities and embryo death. The greatest number of abnormalities resulted within five hours following irradiation, but increased through 20 hours post-exposure. At doses above 300 rads, the magnitude of damage was greater in the in vitro embryos than that shown in previous in vivo studies." @default.
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- W1996602885 date "1973-08-01" @default.
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- W1996602885 title "Radiation induced abnormalities in early in vitro mouse embryos" @default.
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- W1996602885 doi "https://doi.org/10.1002/ar.1091760404" @default.
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