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- W1996605440 abstract "An enzyme-linked immunosorbent assay for measuring penicillin groups is described. Alkaline phosphatase conjugated to penicilloyl residue was reacted with purified anti-penicilloyl antibodies coated upon polystyrene plates. The inhibition of this reaction allowed a specific determination of penicilloyl residues at the picomole level. This assay was applied to the study of the penicilloyl groups linked to human albumin and to the measurement of some of these groups after enzymatic degradation or physical modifications of the albumin molecule. Une technique immunoenzymatique de type ELISA a été mise au point pour mesurer les groupements pénicilloyls. Cette méthode est basée sur la réaction de la phosphatase alcaline pénicilloylée avec des anticorps anti-pénicilloyls purifiés, fixés sur des plaques de polystyrène. L'inhibition de cette réaction permet une détermination spécifique de résidues pénicilloyls avec une sensibilité de l'ordre du picomole. Cette méthode a été utilisée pour étudier la fixation des groupements pénicilloyls sur l'albumine humaine et le démasquage d'une partie de ces groupements après dégradation enzymatique ou modifications physiques de la molécule d'albumine." @default.
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- W1996605440 date "1985-01-01" @default.
- W1996605440 modified "2023-09-25" @default.
- W1996605440 title "Enzyme-linked immunosorbent assayfor measurement of penicilloyl groups" @default.
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- W1996605440 doi "https://doi.org/10.1016/s0769-2625(85)80007-6" @default.
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