Matches in SemOpenAlex for { <https://semopenalex.org/work/W1996608964> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W1996608964 endingPage "719" @default.
- W1996608964 startingPage "715" @default.
- W1996608964 abstract "To develop a system for gene replacement in Nocardia corallina, a protocol for electroporation was optimized by systematic alterations of growth conditions, field strength, time constant and the electroporation buffer. Transformation efficiencies of 0.5 × 10 6 –3 × 10 6 transformants/μg plasmid DNA were obtained routinely. The gene encoding the polyhydroxyalkanoate (PHA) synthase I of N. corallina was cloned and interrupted by insertion of a kanamycin-resistance gene. The resulting plasmid was introduced into N. corallina by electroporation to inactivate the wild-type gene by homologous recombination. Kanamycin-resistant clones were screened by Southern hybridization for the absence of the wild-type gene and analyzed for PHA accumulation.Key words: gene replacement, Nocardia, polyhydroxyalkanoates (PHAs)." @default.
- W1996608964 created "2016-06-24" @default.
- W1996608964 creator A5038481779 @default.
- W1996608964 creator A5042583226 @default.
- W1996608964 date "1996-07-01" @default.
- W1996608964 modified "2023-09-23" @default.
- W1996608964 title "Application of an optimized electroporation procedure for replacement of the polyhydroxyalkanoate synthase I gene inNocardia corallina" @default.
- W1996608964 cites W1716611897 @default.
- W1996608964 cites W1888445893 @default.
- W1996608964 cites W1928450561 @default.
- W1996608964 cites W1972842851 @default.
- W1996608964 cites W2019521033 @default.
- W1996608964 cites W2083555198 @default.
- W1996608964 cites W2089138855 @default.
- W1996608964 cites W2101492502 @default.
- W1996608964 cites W2106675001 @default.
- W1996608964 cites W2125200752 @default.
- W1996608964 cites W2155155225 @default.
- W1996608964 cites W2159613263 @default.
- W1996608964 cites W2167113833 @default.
- W1996608964 doi "https://doi.org/10.1139/m96-098" @default.
- W1996608964 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8764686" @default.
- W1996608964 hasPublicationYear "1996" @default.
- W1996608964 type Work @default.
- W1996608964 sameAs 1996608964 @default.
- W1996608964 citedByCount "3" @default.
- W1996608964 crossrefType "journal-article" @default.
- W1996608964 hasAuthorship W1996608964A5038481779 @default.
- W1996608964 hasAuthorship W1996608964A5042583226 @default.
- W1996608964 hasConcept C102744134 @default.
- W1996608964 hasConcept C104317684 @default.
- W1996608964 hasConcept C153911025 @default.
- W1996608964 hasConcept C186852380 @default.
- W1996608964 hasConcept C204241405 @default.
- W1996608964 hasConcept C22744801 @default.
- W1996608964 hasConcept C2780920689 @default.
- W1996608964 hasConcept C523546767 @default.
- W1996608964 hasConcept C54355233 @default.
- W1996608964 hasConcept C73447357 @default.
- W1996608964 hasConcept C86803240 @default.
- W1996608964 hasConcept C89423630 @default.
- W1996608964 hasConceptScore W1996608964C102744134 @default.
- W1996608964 hasConceptScore W1996608964C104317684 @default.
- W1996608964 hasConceptScore W1996608964C153911025 @default.
- W1996608964 hasConceptScore W1996608964C186852380 @default.
- W1996608964 hasConceptScore W1996608964C204241405 @default.
- W1996608964 hasConceptScore W1996608964C22744801 @default.
- W1996608964 hasConceptScore W1996608964C2780920689 @default.
- W1996608964 hasConceptScore W1996608964C523546767 @default.
- W1996608964 hasConceptScore W1996608964C54355233 @default.
- W1996608964 hasConceptScore W1996608964C73447357 @default.
- W1996608964 hasConceptScore W1996608964C86803240 @default.
- W1996608964 hasConceptScore W1996608964C89423630 @default.
- W1996608964 hasIssue "7" @default.
- W1996608964 hasLocation W19966089641 @default.
- W1996608964 hasLocation W19966089642 @default.
- W1996608964 hasOpenAccess W1996608964 @default.
- W1996608964 hasPrimaryLocation W19966089641 @default.
- W1996608964 hasRelatedWork W1568433874 @default.
- W1996608964 hasRelatedWork W1805017132 @default.
- W1996608964 hasRelatedWork W1994732733 @default.
- W1996608964 hasRelatedWork W1996608964 @default.
- W1996608964 hasRelatedWork W2042652537 @default.
- W1996608964 hasRelatedWork W2055328006 @default.
- W1996608964 hasRelatedWork W2089454290 @default.
- W1996608964 hasRelatedWork W2092285653 @default.
- W1996608964 hasRelatedWork W2149223141 @default.
- W1996608964 hasRelatedWork W2436584291 @default.
- W1996608964 hasVolume "42" @default.
- W1996608964 isParatext "false" @default.
- W1996608964 isRetracted "false" @default.
- W1996608964 magId "1996608964" @default.
- W1996608964 workType "article" @default.