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• W1996627547 startingPage "2673" @default.
• W1996627547 abstract "ABSTRACT Clonogenic (single-cell plating) assays were used to define and quantify subpopulations of two genetically closely related variants of influenza virus A/TK/OR/71 that differed primarily in the size of the NS1 gene product; they expressed a full-size (amino acids [aa] 1 to 230) or truncated (aa 1 to 124) NS1 protein. Monolayers of Vero cells were infected with different amounts of virus, monodispersed, and plated. Cell survival curves were generated from the fraction of cells that produced visible colonies as a function of virus multiplicity. The exponential loss of colony-forming capacity at low multiplicities demonstrated that a single virus particle sufficed to kill a cell. The ratios of cell-killing particles (CKP) to plaque-forming particles (PFP) were 1:1 and 7:1 in populations of variants NS1 1-124 and NS1 1-230 , respectively. This study revealed a new class of particles in influenza virus populations—noninfectious CKP. Both infectious and noninfectious CKP were 6.3 times more resistant to UV radiation than PFP activity. Based on UV target theory, a functional polymerase subunit was implicated in a rate-limiting step in cell killing. Since influenza viruses kill cells by apoptosis (programmed cell death), CKP are functionally apoptosis-inducing particles. Noninfectious CKP are present in excess of PFP in virus populations with full-size NS1 and induce apoptosis that is temporally delayed and morphologically different than that initiated by infectious CKP present in the virus population expressing truncated NS1. The identification and quantification of both infectious and noninfectious CKP defines new phenotypes in influenza virus populations and presents a challenge to determine their role in regulating infectivity, pathogenesis, and vaccine efficacy." @default.
• W1996627547 created "2016-06-24" @default.
• W1996627547 creator A5005716398 @default.
• W1996627547 creator A5043115470 @default.
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• W1996627547 date "2008-03-15" @default.
• W1996627547 modified "2023-10-16" @default.
• W1996627547 title "Clonogenic Assay of Type A Influenza Viruses Reveals Noninfectious Cell-Killing (Apoptosis-Inducing) Particles" @default.
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• W1996627547 doi "https://doi.org/10.1128/jvi.02221-07" @default.
• W1996627547 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2258965" @default.
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• W1996627547 hasPublicationYear "2008" @default.
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