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• W1996848395 abstract "We describe a method leading to the formation of closed circles of rDNA starting from total DNA of Xenopus laevis. Linear DNA molecules were digested with exonuclease 3 and self-annealed. Open circles were enriched and covalently closed by the simultaneous use of polynucleotide kinase, DNA polymerase and polynucleotide ligase. Closed circles of rDNA1 were shown to be alkali-resistant, to have higher density than linear molecules in cesium chloride density gradients containing ethydium bromide, and to have the sedimentation constant expected for a single repeat unit of rDNA comprehensive of its spacer." @default.
• W1996848395 created "2016-06-24" @default.
• W1996848395 creator A5033331383 @default.
• W1996848395 creator A5047462853 @default.
• W1996848395 date "1978-01-01" @default.
• W1996848395 modified "2023-09-23" @default.
• W1996848395 title "Preparation and isolation of covalently closed circular rDNA molecules from DNA of Xenopus laevis" @default.
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• W1996848395 doi "https://doi.org/10.1093/nar/5.6.2211" @default.
• W1996848395 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/342154" @default.
• W1996848395 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/673851" @default.
• W1996848395 hasPublicationYear "1978" @default.
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