FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1996862854> ?p ?o ?g. }

• W1996862854 endingPage "231" @default.
• W1996862854 startingPage "215" @default.
• W1996862854 abstract "Recently, we designed a fast and simple method to obtain nuclei for the alkaline SCG assay and we tested it with mouse liver, lung, kidney, spleen, and bone marrow. Instead of isolating organ cells by trypsinization, we homogenized tissue and isolated the nuclei. Each organ was minced, and the mince was suspended in chilled homogenizing buffer containing NaCl and Na2EDTA, homogenized gently using a Potter-type homogenizer set in ice, and then centrifuged. The nuclei from the precipitate were used for the assay. To evaluate the validity of this method, we tested the genotoxicity in mouse organs of 11 chemical mutagens with different modes of action. Mice were sacrificed 3 and 24 h after administration of each mutagen. Treatment with three alkylating agents (MMS, EMS, and MNNG), a DNA crosslinking agent (MMC), two aromatic amines (2-AAF and phenacetin), a polycyclic aromatic hydrocarbon (B[a]P), and two inorganic chemicals (KBrO3 and K2CrO4) increased migration of the DNA from mouse organs. 5-FU (a base analog) and colchicine (a spindle poison) treatment produced negative results in all organ studied. Considering that the alkaline SCG assay detects genotoxicity as DNA fragments derived from DNA single-strand breaks and alkali-labile damage, our results showed that the SCG assay using our homogenization technique detected chemical mutagens as a function of their modes of action." @default.
• W1996862854 created "2016-06-24" @default.
• W1996862854 creator A5013859058 @default.
• W1996862854 creator A5021926265 @default.
• W1996862854 creator A5033283500 @default.
• W1996862854 creator A5050264696 @default.
• W1996862854 creator A5071129667 @default.
• W1996862854 date "1997-07-01" @default.
• W1996862854 modified "2023-09-29" @default.
• W1996862854 title "Simple detection of chemical mutagens by the alkaline single-cell gel electrophoresis (Comet) assay in multiple mouse organs (liver, lung, spleen, kidney, and bone marrow)" @default.
• W1996862854 cites W1556134092 @default.
• W1996862854 cites W1966016371 @default.
• W1996862854 cites W1989790202 @default.
• W1996862854 cites W1995920508 @default.
• W1996862854 cites W2009747449 @default.
• W1996862854 cites W2025858923 @default.
• W1996862854 cites W2036307672 @default.
• W1996862854 cites W2036347479 @default.
• W1996862854 cites W2048780820 @default.
• W1996862854 cites W2074496226 @default.
• W1996862854 cites W2074852426 @default.
• W1996862854 cites W2076198146 @default.
• W1996862854 cites W2081501219 @default.
• W1996862854 cites W2089337036 @default.
• W1996862854 cites W2092629964 @default.
• W1996862854 cites W4234736483 @default.
• W1996862854 doi "https://doi.org/10.1016/s1383-5718(97)00073-9" @default.
• W1996862854 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9268047" @default.
• W1996862854 hasPublicationYear "1997" @default.
• W1996862854 type Work @default.
• W1996862854 sameAs 1996862854 @default.
• W1996862854 citedByCount "106" @default.
• W1996862854 countsByYear W19968628542012 @default.
• W1996862854 countsByYear W19968628542013 @default.
• W1996862854 countsByYear W19968628542014 @default.
• W1996862854 countsByYear W19968628542015 @default.
• W1996862854 countsByYear W19968628542016 @default.
• W1996862854 countsByYear W19968628542017 @default.
• W1996862854 countsByYear W19968628542018 @default.
• W1996862854 countsByYear W19968628542019 @default.
• W1996862854 countsByYear W19968628542020 @default.
• W1996862854 countsByYear W19968628542021 @default.
• W1996862854 countsByYear W19968628542022 @default.
• W1996862854 crossrefType "journal-article" @default.
• W1996862854 hasAuthorship W1996862854A5013859058 @default.
• W1996862854 hasAuthorship W1996862854A5021926265 @default.
• W1996862854 hasAuthorship W1996862854A5033283500 @default.
• W1996862854 hasAuthorship W1996862854A5050264696 @default.
• W1996862854 hasAuthorship W1996862854A5071129667 @default.
• W1996862854 hasConcept C116084860 @default.
• W1996862854 hasConcept C134018914 @default.
• W1996862854 hasConcept C143425029 @default.
• W1996862854 hasConcept C153911025 @default.
• W1996862854 hasConcept C178790620 @default.
• W1996862854 hasConcept C185592680 @default.
• W1996862854 hasConcept C203014093 @default.
• W1996862854 hasConcept C2776639595 @default.
• W1996862854 hasConcept C2778959862 @default.
• W1996862854 hasConcept C2780007613 @default.
• W1996862854 hasConcept C2780091579 @default.
• W1996862854 hasConcept C2780358455 @default.
• W1996862854 hasConcept C2780931953 @default.
• W1996862854 hasConcept C29730261 @default.
• W1996862854 hasConcept C552990157 @default.
• W1996862854 hasConcept C55493867 @default.
• W1996862854 hasConcept C86803240 @default.
• W1996862854 hasConceptScore W1996862854C116084860 @default.
• W1996862854 hasConceptScore W1996862854C134018914 @default.
• W1996862854 hasConceptScore W1996862854C143425029 @default.
• W1996862854 hasConceptScore W1996862854C153911025 @default.
• W1996862854 hasConceptScore W1996862854C178790620 @default.
• W1996862854 hasConceptScore W1996862854C185592680 @default.
• W1996862854 hasConceptScore W1996862854C203014093 @default.
• W1996862854 hasConceptScore W1996862854C2776639595 @default.
• W1996862854 hasConceptScore W1996862854C2778959862 @default.
• W1996862854 hasConceptScore W1996862854C2780007613 @default.
• W1996862854 hasConceptScore W1996862854C2780091579 @default.
• W1996862854 hasConceptScore W1996862854C2780358455 @default.
• W1996862854 hasConceptScore W1996862854C2780931953 @default.
• W1996862854 hasConceptScore W1996862854C29730261 @default.
• W1996862854 hasConceptScore W1996862854C552990157 @default.
• W1996862854 hasConceptScore W1996862854C55493867 @default.
• W1996862854 hasConceptScore W1996862854C86803240 @default.
• W1996862854 hasIssue "3" @default.
• W1996862854 hasLocation W19968628541 @default.
• W1996862854 hasLocation W19968628542 @default.
• W1996862854 hasOpenAccess W1996862854 @default.
• W1996862854 hasPrimaryLocation W19968628541 @default.
• W1996862854 hasRelatedWork W1586066921 @default.
• W1996862854 hasRelatedWork W1855115129 @default.
• W1996862854 hasRelatedWork W1970577073 @default.
• W1996862854 hasRelatedWork W1999735484 @default.
• W1996862854 hasRelatedWork W2010127944 @default.
• W1996862854 hasRelatedWork W2059632900 @default.
• W1996862854 hasRelatedWork W2347883925 @default.
• W1996862854 hasRelatedWork W2955796482 @default.
• W1996862854 hasRelatedWork W3022454422 @default.
• W1996862854 hasRelatedWork W4281841367 @default.

• next