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- W1996918042 abstract "Abstract The uptake and metabolism of the pyridazinone herbicide metflurazon by the green alga Chlorella fusca was studied using a new and sensitive technique which is based on reverse-phase high-performance liquid chromatography. During the metflurazon-induced bleaching process, the agent is rapidly taken up and metabolized to the monomethyl derivative, norflurazon, by N -demethylation. Norflurazon accumulates during the treatment in the culture medium because Chlorella cells demethylate norflurazon to the less-phytotoxic SAN 9774 only to a very minor extent. It is shown that only 10% of the initial 1 μ M metflurazon is sufficient to induce the bleaching process. This amount is much too small to directly inhibit the photosynthetic electron transport, so that a contribution of this site of action to the bleaching process is not likely. Treated Chlorella cells are able to regenerate to photosynthetically active cells. This phenomenon is based on the inability of the regenerating algae to further take up and demethylate metflurazon. Fully regenerated cells are resistant to metflurazon because a treatment with 1 μ M metflurazon neither affects growth nor inhibits photosynthetic activity. This resistance results from an altered permeability of the cell membrane with respect to phenylpyridazinones and an inhibited N -demethylation of metflurazon. These results clearly show that metflurazon itself is nonphytotoxic, and has to be demethylated to norflurazon to exert its bleaching activity." @default.
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- W1996918042 date "1984-10-01" @default.
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- W1996918042 title "Uptake and metabolism of the phenylpyridazinone herbicide metflurazon during the bleaching and regeneration process of the green alga, Chlorella fusca" @default.
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- W1996918042 doi "https://doi.org/10.1016/0048-3575(84)90093-2" @default.
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