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• W1996989293 endingPage "14532" @default.
• W1996989293 startingPage "14521" @default.
• W1996989293 abstract "Sphingomyelin is a lipid that is abundant in the nervous systems of mammals, where it is associated with putative microdomains in cellular membranes and undergoes alterations due to aging or neurodegeneration. We investigated the effect of varying the concentration of cholesterol in binary and ternary mixtures with N-palmitoylsphingomyelin (PSM) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) using deuterium nuclear magnetic resonance ((2)H NMR) spectroscopy in both macroscopically aligned and unoriented multilamellar dispersions. In our experiments, we used PSM and POPC perdeuterated on the N-acyl and sn-1 acyl chains, respectively. By measuring solid-state (2)H NMR spectra of the two lipids separately in mixtures with the same compositions as a function of cholesterol mole fraction and temperature, we obtained clear evidence for the coexistence of two liquid-crystalline domains in distinct regions of the phase diagram. According to our analysis of the first moments M1 and the observed (2)H NMR spectra, one of the domains appears to be a liquid-ordered phase. We applied a mean-torque potential model as an additional tool to calculate the average hydrocarbon thickness, the area per lipid, and structural parameters such as chain extension and thermal expansion coefficient in order to further define the two coexisting phases. Our data imply that phase separation takes place in raftlike ternary PSM/POPC/cholesterol mixtures over a broad temperature range but vanishes at cholesterol concentrations equal to or greater than a mole fraction of 0.33. Cholesterol interacts preferentially with sphingomyelin only at smaller mole fractions, above which a homogeneous liquid-ordered phase is present. The reasons for these phase separation phenomena seem to be differences in the effects of cholesterol on the configurational order of the palmitoyl chains in PSM-d31 and POPC-d31 and a difference in the affinity of cholesterol for sphingomyelin observed at low temperatures. Hydrophobic matching explains the occurrence of raftlike domains in cellular membranes at intermediate cholesterol concentrations but not saturating amounts of cholesterol." @default.
• W1996989293 created "2016-06-24" @default.
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• W1996989293 creator A5054829961 @default.
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• W1996989293 date "2008-10-08" @default.
• W1996989293 modified "2023-09-25" @default.
• W1996989293 title "Raftlike Mixtures of Sphingomyelin and Cholesterol Investigated by Solid-State ²H NMR Spectroscopy" @default.
• W1996989293 cites W1498171831 @default.
• W1996989293 cites W1500080863 @default.
• W1996989293 cites W1503369148 @default.
• W1996989293 cites W1563035509 @default.
• W1996989293 cites W1784927340 @default.
• W1996989293 cites W1968682299 @default.
• W1996989293 cites W1969141277 @default.
• W1996989293 cites W1975027906 @default.
• W1996989293 cites W1980478162 @default.
• W1996989293 cites W1980584512 @default.
• W1996989293 cites W1984981562 @default.
• W1996989293 cites W1987033415 @default.
• W1996989293 cites W1987263403 @default.
• W1996989293 cites W1987455793 @default.
• W1996989293 cites W1988047008 @default.
• W1996989293 cites W1988201012 @default.
• W1996989293 cites W1990912620 @default.
• W1996989293 cites W1991508565 @default.
• W1996989293 cites W1994878489 @default.
• W1996989293 cites W1995093903 @default.
• W1996989293 cites W1995902365 @default.
• W1996989293 cites W1996008402 @default.
• W1996989293 cites W1998634209 @default.
• W1996989293 cites W2009559464 @default.
• W1996989293 cites W2011915654 @default.
• W1996989293 cites W2012184845 @default.
• W1996989293 cites W2015943547 @default.
• W1996989293 cites W2021566521 @default.
• W1996989293 cites W2021882008 @default.
• W1996989293 cites W2023502440 @default.
• W1996989293 cites W2024156287 @default.
• W1996989293 cites W2024372419 @default.
• W1996989293 cites W2024988532 @default.
• W1996989293 cites W2025778038 @default.
• W1996989293 cites W2026578933 @default.
• W1996989293 cites W2030560811 @default.
• W1996989293 cites W2034101835 @default.
• W1996989293 cites W2035025388 @default.
• W1996989293 cites W2040607876 @default.
• W1996989293 cites W2042284982 @default.
• W1996989293 cites W2042574278 @default.
• W1996989293 cites W2043568015 @default.
• W1996989293 cites W2048072189 @default.
• W1996989293 cites W2049382860 @default.
• W1996989293 cites W2058273629 @default.
• W1996989293 cites W2058930537 @default.
• W1996989293 cites W2059596516 @default.
• W1996989293 cites W2061357370 @default.
• W1996989293 cites W2065061795 @default.
• W1996989293 cites W2065158535 @default.
• W1996989293 cites W2065730608 @default.
• W1996989293 cites W2069568296 @default.
• W1996989293 cites W2069919293 @default.
• W1996989293 cites W2072366248 @default.
• W1996989293 cites W2072558995 @default.
• W1996989293 cites W2073059665 @default.
• W1996989293 cites W2074729965 @default.
• W1996989293 cites W2075289114 @default.
• W1996989293 cites W2076854323 @default.
• W1996989293 cites W2076918344 @default.
• W1996989293 cites W2080054653 @default.
• W1996989293 cites W2080171651 @default.
• W1996989293 cites W2084153702 @default.
• W1996989293 cites W2092296126 @default.
• W1996989293 cites W2095454778 @default.
• W1996989293 cites W2106704490 @default.
• W1996989293 cites W2108364101 @default.
• W1996989293 cites W2111393517 @default.
• W1996989293 cites W2113456505 @default.
• W1996989293 cites W2117022351 @default.
• W1996989293 cites W2120345534 @default.
• W1996989293 cites W2121936687 @default.
• W1996989293 cites W2122882997 @default.
• W1996989293 cites W2133388060 @default.
• W1996989293 cites W2133991651 @default.
• W1996989293 cites W2134723427 @default.
• W1996989293 cites W2139135024 @default.
• W1996989293 cites W2142083551 @default.
• W1996989293 cites W2143729332 @default.
• W1996989293 cites W2147692698 @default.
• W1996989293 cites W2149446906 @default.
• W1996989293 cites W2149628585 @default.
• W1996989293 cites W2154070195 @default.
• W1996989293 cites W2182056831 @default.
• W1996989293 cites W2952537573 @default.
• W1996989293 cites W4256062136 @default.
• W1996989293 doi "https://doi.org/10.1021/ja801789t" @default.
• W1996989293 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2756786" @default.

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