Matches in SemOpenAlex for { <https://semopenalex.org/work/W1996992021> ?p ?o ?g. }
Showing items 1 to 70 of
70
with 100 items per page.
- W1996992021 endingPage "554" @default.
- W1996992021 startingPage "551" @default.
- W1996992021 abstract "Studies of the human defensins have been hampered by the lack of a simple expression system allowing for rapid production of functional peptide forms. Here, we describe a Saccharomyces cerevisiae AH22 expression system that meets that condition. The 42 amino acid form of human beta-defensin-1 was expressed under the control of the ADH1 promoter. The optimum conditions for expression were determined and the stable maintenance of the pVT103L-hBD-1 chimeric vector in the yeast population was confirmed. Expressed hBD-1 was secreted into the medium (approximately 55 microg l(-1)) and purified using cation-exchange chromatography. Isolated defensin exhibited strong bactericidal effect on Escherichia coli ML-35p. We conclude that the expression system described here will be a useful tool where readily prepared and active forms of the human defensins are needed." @default.
- W1996992021 created "2016-06-24" @default.
- W1996992021 creator A5040246358 @default.
- W1996992021 creator A5043446577 @default.
- W1996992021 date "2005-08-01" @default.
- W1996992021 modified "2023-09-27" @default.
- W1996992021 title "Production of the Human-Beta-Defensin Using Saccharomyces cerevisiae as a Host" @default.
- W1996992021 doi "https://doi.org/10.2174/0929866054395761" @default.
- W1996992021 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16101394" @default.
- W1996992021 hasPublicationYear "2005" @default.
- W1996992021 type Work @default.
- W1996992021 sameAs 1996992021 @default.
- W1996992021 citedByCount "14" @default.
- W1996992021 countsByYear W19969920212012 @default.
- W1996992021 countsByYear W19969920212013 @default.
- W1996992021 countsByYear W19969920212017 @default.
- W1996992021 countsByYear W19969920212019 @default.
- W1996992021 countsByYear W19969920212020 @default.
- W1996992021 countsByYear W19969920212022 @default.
- W1996992021 crossrefType "journal-article" @default.
- W1996992021 hasAuthorship W1996992021A5040246358 @default.
- W1996992021 hasAuthorship W1996992021A5043446577 @default.
- W1996992021 hasConcept C104317684 @default.
- W1996992021 hasConcept C129437159 @default.
- W1996992021 hasConcept C144024400 @default.
- W1996992021 hasConcept C149923435 @default.
- W1996992021 hasConcept C185592680 @default.
- W1996992021 hasConcept C2776498113 @default.
- W1996992021 hasConcept C2777576037 @default.
- W1996992021 hasConcept C2779222958 @default.
- W1996992021 hasConcept C2779281246 @default.
- W1996992021 hasConcept C2908647359 @default.
- W1996992021 hasConcept C547475151 @default.
- W1996992021 hasConcept C55493867 @default.
- W1996992021 hasConcept C86803240 @default.
- W1996992021 hasConceptScore W1996992021C104317684 @default.
- W1996992021 hasConceptScore W1996992021C129437159 @default.
- W1996992021 hasConceptScore W1996992021C144024400 @default.
- W1996992021 hasConceptScore W1996992021C149923435 @default.
- W1996992021 hasConceptScore W1996992021C185592680 @default.
- W1996992021 hasConceptScore W1996992021C2776498113 @default.
- W1996992021 hasConceptScore W1996992021C2777576037 @default.
- W1996992021 hasConceptScore W1996992021C2779222958 @default.
- W1996992021 hasConceptScore W1996992021C2779281246 @default.
- W1996992021 hasConceptScore W1996992021C2908647359 @default.
- W1996992021 hasConceptScore W1996992021C547475151 @default.
- W1996992021 hasConceptScore W1996992021C55493867 @default.
- W1996992021 hasConceptScore W1996992021C86803240 @default.
- W1996992021 hasIssue "6" @default.
- W1996992021 hasLocation W19969920211 @default.
- W1996992021 hasLocation W19969920212 @default.
- W1996992021 hasOpenAccess W1996992021 @default.
- W1996992021 hasPrimaryLocation W19969920211 @default.
- W1996992021 hasRelatedWork W1997647805 @default.
- W1996992021 hasRelatedWork W2009810460 @default.
- W1996992021 hasRelatedWork W2026244176 @default.
- W1996992021 hasRelatedWork W2062385823 @default.
- W1996992021 hasRelatedWork W2087346710 @default.
- W1996992021 hasRelatedWork W2164285431 @default.
- W1996992021 hasRelatedWork W2343067289 @default.
- W1996992021 hasRelatedWork W2606340556 @default.
- W1996992021 hasRelatedWork W2756564268 @default.
- W1996992021 hasRelatedWork W4251454643 @default.
- W1996992021 hasVolume "12" @default.
- W1996992021 isParatext "false" @default.
- W1996992021 isRetracted "false" @default.
- W1996992021 magId "1996992021" @default.
- W1996992021 workType "article" @default.