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- W1996996914 abstract "Abstract A fluorometric method for quantitating the amount of α- l -fucose enzymatically released from porcine submaxillary mucin (A+) has been developed. The released fucose is oxidized with fucose dehydrogenase and NAD, and the NADH produced, in conjunction with diaphorase is then used to convert the nonfluorescent dye, resazurin, to the highly fluorescent product, resorufin. The method can detect as little as 0.05 nmol of α- l -fucose. α- l -Fucosidase from the mollusc Mercenaria mercenaria is employed for the hydrolysis reaction. The enzymatic release of fucose is maximal to pH 2.85 and, the reaction rate is linearly proportional to incubation time for 20 min under the conditions employed. The presence of fucose in the reaction mixture, after hydrolysis has occurred, is demonstrated by paper chromatography. This procedure provides a rapid, simple, and specific method for quantitating the release of α- l -fucose from porcine submaxillary mucin type A+." @default.
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- W1996996914 date "1981-03-01" @default.
- W1996996914 modified "2023-09-27" @default.
- W1996996914 title "A fluorometric method for quantitating the enzymatic hydrolysis of fucose from porcine submaxillary mucin (A+)" @default.
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- W1996996914 doi "https://doi.org/10.1016/0003-2697(81)90262-1" @default.
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